Recombinant Mouse Anti-IAV HA heptapeptide (DVPDYAS) Antibody (17/9) (CAT#: PABZ-078)

Figure 1 Profiles of reactivity of MAb 17/9 to cell lysates bearing WT or amino acid-substituted MPER-TM1 constructs.

Constructs containing indicated single amino acid substitutions were expressed in COS-7 cells. Cells were lysed, and lysates were produced by sonication and immobilized in microtiter wells. These were probed with 10 nM MAb 17/9, which binds the HA tag (YPYDVPDYA) at the N terminus of each MPER construct. 17/9 MAb with goat (anti-mouse IgG)-HRP (1:1,000 dilution). Abs were diluted in TBS containing 5% NFDM and 0.1% Tween 20. Binding of Nt MAbs, normalized to HA expression in order to account for differences in cell surface expression, is expressed as a percentage of binding to the WT MPER-TM1 construct (black bar; dashed line).

Montero, M., Gulzar, N., Klaric, K. A., Donald, J. E., Lepik, C., Wu, S., ... & Scott, J. K. (2012). Neutralizing epitopes in the membrane-proximal external region of HIV-1 gp41 are influenced by the transmembrane domain and the plasma membrane. Journal of virology, 86(6), 2930-2941.