Recombinant Mouse Antibody (26/9) is capable of binding to IAV HA1, expressed in Chinese Hamster Ovary cells (CHO). This antibody recognizes the same six-residue epitope of an immunogenic peptide from influenza virus hemagglutinin (HA1; 75-110).
Figure 1 Binding of mAb 26/9 to substitution analogs of the control peptide Ace-YPYDVPDYASLRS-NH2.
Direct ELISA experiments (A) using mAb 26/9 and substitution analogs in positions -DVPDYAS- are shown graphically. The percentage inhibition of the control peptide is plotted against the amino acid substitution at each position in the epitope. The table in B shows the IC₅₀ and RF values calculated from the competitive ELISA experiments for 26/9.
Churchill, M. E., Stura, E. A., Pinilla, C., Appel, J. R., Houghten, R. A., Kono, D. H., ... & Wilson, I. A. (1994). Crystal structure of a peptide complex of anti-influenza peptide antibody Fab 26/9: comparison of two different antibodies bound to the same peptide antigen. Journal of molecular biology, 241(4), 534-556.
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