Does this antibody inhibit IL-15 binding?

Yes, this recombinant human antibody (clone DISC0280) not only inhibits the binding of IL-15 to the IL-15 alpha-receptor subunit but is also capable of blocking the signaling of IL-15 through these subunits in functional assays.

Human Anti-IL15 Recombinant Antibody (clone DISC0280)

CAT#: PABZ-081

Recombinant Human Antibody (DISC0280) is capable of binding to IL15, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-IL15 mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-IL15 mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. This antibody not only inhibits the binding of IL-15 to the IL-15 α-receptor subunit but also is capable of blocking the signalling of IL-15 through these subunits.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line A549

Immunofluorescent staining of human cell line A549 shows localization to nuclear speckles & cytosol.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37738/if_selected.jpg

Subcellular Location Figure 2 IHC staining of human lymph node

Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in reaction center cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4457/ihc_selected.jpg

Subcellular Location Figure 3 IF staining of human cell line HeLa

Immunofluorescent staining of human cell line HeLa shows localization to nuclear speckles & cytosol.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37738/785_B10_1_red_green.jpg

Subcellular Location Figure 4 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles & cytosol.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37738/783_B10_1_red_green.jpg

Normal Tissue Figure 5 Cerebral cortex

Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4457/15421_B_8_5.jpg

Normal Tissue Figure 6 Lung

Macrophages
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4457/15421_A_1_4.jpg

Normal Tissue Figure 7 Colon

Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4457/15421_A_8_3.jpg

Normal Tissue Figure 8 Testis

Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4457/15421_A_6_6.jpg

Normal Tissue Figure 9 Lymph node

Germinal center cells
Staining:

High

Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
nuclear
Non-germinal center cells
Staining:

High

Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4457/15421_A_9_8.jpg

RNA Expression Figure 10 RNA cell line category: Cell line enhanced (HDLM-2, Karpas-707)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000164136-IL15

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Specifications

Immunogen
Human interleukin 15

Host Species
Human

Type
Human IgG

Specificity
Human IL15

Species Reactivity
Human

Clone
DISC0280

Applications
Neut

Product Property

Purity
>95% as determined by SDS-PAGE and HPLC analysis

Concentration
Please refer to the vial label for the specific concentration.

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
The antibody DISC0280 has been reported in applications of Inhib, FuncS, Neut. It's recommended that the optimal antibody concentration, dilution, incubition time etc. are best to be carefully titrated in specific assays.

Target

Alternative Names
IL15; interleukin 15; IL-15; interleukin-15

Gene ID
3600

UniProt ID
P40933

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Breast cancer biomarkers at key points during disease progression

Blocks IL-15 Signaling

This antibody (clone DISC0280) is highly effective at blocking IL-15 signaling through the alpha-receptor subunit. We used it in neutralization assays, and it significantly inhibited the cytokine's activity. A very potent tool for cytokine research.

High Purity Recombinant Antibody

The quality of this human anti-IL15 antibody is excellent. Purity analysis by SDS-PAGE and HPLC showed greater than 95%. It exists as a disulfide-linked dimer, which we found to be stable for our functional studies.

Q&As

Does this antibody inhibit IL-15 binding?

A: Yes, this recombinant human antibody (clone DISC0280) not only inhibits the binding of IL-15 to the IL-15 alpha-receptor subunit but is also capable of blocking the signaling of IL-15 through these subunits in functional assays.
What is the structure of this antibody?

A: It is expressed as a disulfide-linked dimer of a heavy chain (HC) containing VH from anti-IL15 mAb and CH1-3 region of human IgG1, and a light chain (LC) encoding VL from anti-IL15 mAb and CL of human kappa light chain.

View the frequently asked questions answered by Creative Biolabs Support.

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Related Diseases

Rheumatoid Arthritis

Related Signaling Pathways

TNF Signaling Pathway

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - PABZ-081.pdf

MSDS

MSDS - PABZ-081.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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