Human Anti-IL15 Recombinant Antibody (clone DISC0280) (CAT#: PABZ-081)
Recombinant Human Antibody (DISC0280) is capable of binding to IL15, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-IL15 mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-IL15 mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. This antibody not only inhibits the binding of IL-15 to the IL-15 α-receptor subunit but also is capable of blocking the signalling of IL-15 through these subunits.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A549
(Immunofluorescent staining of human cell line A549 shows localization to nuclear speckles & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37738/if_selected.jpg
(Immunofluorescent staining of human cell line A549 shows localization to nuclear speckles & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37738/if_selected.jpg
Figure 2 IHC staining of human lymph node
(Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in reaction center cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4457/ihc_selected.jpg
(Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in reaction center cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4457/ihc_selected.jpg
Figure 3 IF staining of human cell line HeLa
(Immunofluorescent staining of human cell line HeLa shows localization to nuclear speckles & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37738/785_B10_1_red_green.jpg
(Immunofluorescent staining of human cell line HeLa shows localization to nuclear speckles & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37738/785_B10_1_red_green.jpg
Figure 4 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37738/783_B10_1_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37738/783_B10_1_red_green.jpg
Figure 5 Cerebral cortex
(Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4457/15421_B_8_5.jpg
(Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4457/15421_B_8_5.jpg
Figure 6 Lung
(Macrophages
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4457/15421_A_1_4.jpg
(Macrophages
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4457/15421_A_1_4.jpg
Figure 7 Colon
(Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4457/15421_A_8_3.jpg
(Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4457/15421_A_8_3.jpg
Figure 8 Testis
(Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4457/15421_A_6_6.jpg
(Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4457/15421_A_6_6.jpg
Figure 9 Lymph node
(Germinal center cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
nuclear
Non-germinal center cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4457/15421_A_9_8.jpg
(Germinal center cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
nuclear
Non-germinal center cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4457/15421_A_9_8.jpg
Figure 10 RNA cell line category: Cell line enhanced (HDLM-2, Karpas-707)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000164136-IL15
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000164136-IL15
Figure 10 RNA cell line category: Cell line enhanced (HDLM-2, Karpas-707)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000164136-IL15
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000164136-IL15
Specifications
- Immunogen
- Human interleukin 15
- Host Species
- Human
- Type
- Human IgG
- Specificity
- Human IL15
- Species Reactivity
- Human
- Clone
- DISC0280
- Applications
- Neut
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody DISC0280 has been reported in applications of Inhib, FuncS, Neut. It's recommended that the optimal antibody concentration, dilution, incubition time etc. are best to be carefully titrated in specific assays.
Target
Related Resources
Rheumatoid Arthritis
TNF Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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ScFv-Fc Chimera Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1254z-F(E) | Recombinant Anti-Human IL15 Antibody Fab Fragment | IF, FACS, FuncS | Fab |
| HPAB-S0072-YC-F(E) | Human Anti-IL15 Recombinant Antibody (clone 4H10); Fab Fragment | ELISA | Human Fab |
| HPAB-S0073-YC-F(E) | Human Anti-IL15 Recombinant Antibody (clone 1H3); Fab Fragment | ELISA | Human Fab |
| HPAB-S0074-YC-F(E) | Human Anti-IL15 Recombinant Antibody (clone 3C1); Fab Fragment | ELISA | Human Fab |
| HPAB-S0075-YC-F(E) | Human Anti-IL15 Recombinant Antibody (clone 8C5); Fab Fragment | ELISA | Human Fab |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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