Anti-Human IL5RA Recombinant Antibody (TAB-222) (CAT#: TAB-222)
Recombinant monoclonal antibody to IL5RA. It is a monoclonal antibody for the treatment of asthma. It is directed against the alpha-chain of the interleukin-5 receptor (CD125).
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SDS-PAGE
Figure 1 Anti-Human IL5RA Antibody (TAB-222) in SDS-PAGE
SDS-PAGE analysis of TAB-222 in non-reduced (Lane 1) and reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
HPLC
Figure 2 Anti-Human IL5RA Antibody (TAB-222-LowE) in HPLC
The purity of this antibody is> 95% as determined by SEC-HPLC.
WB
Figure 3 Anti-Human IL5RA Antibody (TAB-222) in WB
Western blot analysis of TAB-222 was performed by loading IL5RA Protein, Human, Recombinant (His Tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-222 and Goat Anti-Human IgG-HRP as a secondary antibody. Chemiluminescent detection was performed.
Lane 1: Reducing antigen (0.1 μg)
Lane 2: Reducing antigen (0.3 μg)
Lane 3: Reducing antigen (0.6 μg)
ELISA
Figure 4 Anti-Human IL5RA Antibody (TAB-222) in ELISA
ELISA analysis of TAB-222 was performed by coating with IL5RA Protein, Human, Recombinant (His Tag). Then blocked with BSA, and incubated with Anti-Human IL5RA Antibody (TAB-222). The Goat Anti-Human IgG-HRP as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
DB
Figure 5 Anti-Human IL5RA Antibody (TAB-222) in DB
Dot Blot analysis of TAB-222 was performed by coating with IL5RA Protein, Human, Recombinant (His Tag).
TAB-222 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-human IgG as a secondary antibody (1: 2000)
Specifications
- Immunogen
- The details of the immunogen for this antibody are not available.
- Host Species
- Mouse
- Derivation
- Humanized (from mouse)
- Type
- IgG1 - kappa
- Specificity
- Tested positive against native human antigen.
- Species Reactivity
- Human
- Applications
- Suitable for use in WB, FC, IP, ELISA, Neut, FuncS, IF and most other immunological methods.
- MW
- 146.0 kDa
- Related Disease
- Asthma
Product Property
- Purity
- >95.0% as determined by analysis by RP-HPLC.
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Target
- Alternative Names
- Benralizumab;1044511-01-4;MEDI-563;BIW-8405;KHK4563;IL5RA;interleukin 5 receptor, alpha;IL5R;interleukin-5 receptor subunit alpha;CD125;CDw125;CD125 antigen;IL-5R subunit alpha;IL-5 receptor subunit alpha;interleukin 5 receptor type 3;interleukin-5 recept
- Gene ID
- 3568
- UniProt ID
- Q01344
Related Resources
Please refer to Benralizumab Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Benralizumab.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2473z | Mouse Anti-IL5RA Recombinant Antibody (clone 33H12) | WB, FC | Mouse IgG1 |
| HPAB-1545WJ | Human Anti-IL5RA Recombinant Antibody (clone KM1399) | ELISA, WB | Human IgG1, κ |
| VS-0723-WK142 | Human Anti-IL5RA Recombinant Antibody (VS-0723-WK142) | FC | Chimeric (rabbit/human) IgG1 |
| VS13-YC584 | Rabbit Anti-IL5RA Recombinant Antibody (VS13-YC584) | IHC-P | Rabbit IgG |
Customer Reviews and Q&As
Customer Review
Q&As
Sarah Johnson
Ideal for IL5RA Research
Ideal for IL5RA Research
The Anti-Human IL5RA Therapeutic Antibody (Benralizumab) has been a fantastic addition to our IL5RA studies. Its specificity and sensitivity are outstanding, consistently providing clear results. This antibody has become essential for our research, improving the accuracy and reliability of our assays.
13.Mar,23
Brian Martinez
Reliable for Immunoassays
Reliable for Immunoassays
We incorporated this antibody into our immunoassays, and it has been a great asset. The specificity for IL5RA is precise, allowing for accurate detection and analysis. The reliable performance has made it an invaluable tool in our lab.
18.Jul,22
Megan Thompson
Consistent and Effective
Consistent and Effective
This antibody has proven to be consistent and effective in our various assays. The high-quality, reproducible results have significantly improved our experiments, making it a crucial part of our research toolkit. The strong affinity and specificity for IL5RA have been particularly beneficial.
11.Aug,21
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-222, RRID: AB_3111882)
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