Mouse Anti-INSR Recombinant Antibody (clone 83-14) (CAT#: PABZ-083)
Recombinant Mouse Antibody (83-14) is capable of binding to INSR, expressed in Chinese Hamster Ovary cells (CHO).
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Figure 1 Insulin- and antibody-stimulated glucose uptake.
Antibody-stimulated glucose uptake. Insulin (30 nM), the indicated monoclonal antibodies (30 nM), or a polyclonal antibody (B-10, diluted 1:150) was added to the transfected CHO cells and incubated 30 min at 370C; deoxyglucose uptake was then measured. Results are expressed as percent stimulation over control cells that did not receive insulin.
Ebina, Y., Araki, E., Taira, M., Shimada, F., Mori, M., Craik, C. S., ... & Rutter, W. J. (1987). Replacement of lysine residue 1030 in the putative ATP-binding region of the insulin receptor abolishes insulin-and antibody-stimulated glucose uptake and receptor kinase activity. Proceedings of the National Academy of Sciences, 84(3), 704-708.
Specifications
- Immunogen
- Human insulin receptor
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human INSR
- Species Reactivity
- Human
- Clone
- 83-14
- Applications
- WB, FuncS
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody 83-14 has been reported in applications of Stim, IP, WB, FuncS. It's recommended that the optimal antibody concentration, dilution, incubition time etc. are best to be carefully titrated in specific assays.
Target
Related Resources
PPAR Signaling Pathway
Type I Diabetes Mellitus
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1236z | Mouse Anti-INSR Recombinant Antibody (clone 25F8) | ELISA, FC, ICC, IF, IHC, FuncS | Mouse IgG1, κ |
| HPAB-2352LY | Human Anti-INSR Recombinant Antibody (HPAB-2352LY) | ELISA, WB, FC, FuncS | Humanized IgG |
| HPAB-2353LY | Human Anti-INSR Recombinant Antibody (HPAB-2353LY) | ELISA, WB | Humanized IgG |
| HPAB-2354LY | Human Anti-INSR Recombinant Antibody (HPAB-2354LY) | ELISA, WB | Humanized IgG |
| HPAB-2355LY | Human Anti-INSR Recombinant Antibody (HPAB-2355LY) | ELISA, WB | Humanized IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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