Human Anti-JAG1 Recombinant Antibody (HPAB-0500-CN) (CAT#: HPAB-0500-CN)
This product is a recombinant human antibody that recognizes JAG1. The antibody was expressed in mammalian cells with chemically defined culture media and was purified by affinity chromatography.
Specific Inquiry
SDS-PAGE
Figure 1 Human Anti-JAG1 Recombinant Antibody (HPAB-0500-CN) in SDS-PAGE
Lane 1: Reduced Antibody
Lane 2: Non-Reduced Antibody
SEC-HPLC
Figure 2 Human Anti-JAG1 Recombinant Antibody (HPAB-0500-CN) in SEC-HPLC
The purity of HPAB-0500-CN was> 87% as determined by SEC-HPLC.
ELISA
Figure 3 Human Anti-JAG1 Recombinant Antibody (HPAB-0500-CN) in ELISA
ELISA analysis of HPAB-0500-CN was performed by coating with human JAG1 protein.
The secondary antibody: HRP-conjugated Goat anti-human IgG
WB
Figure 4 Human Anti-JAG1 Recombinant Antibody (HPAB-0500-CN) in WB
Western blot analysis of HPAB-0500-CN was performed by loading human JAG1 protein onto a 12% Tris-HCl polyacrylamide gel.
HPAB-0500-CN incubation concentration: 2 ng/μL.
The secondary antibody: HRP-conjugated Goat Anti-Human IgG
Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.3 μg)
Lane 3: Reducing Antigen (0.6 μg)
DB
Figure 5 Human Anti-JAG1 Recombinant Antibody (HPAB-0500-CN) in DB
Dot blot analysis of HPAB-0500-CN was performed by loading human JAG1 protein onto a 12% Tris-HCl polyacrylamide gel.
HPAB-0500-CN incubation concentration: 2 ng/μL.
The secondary antibody: HRP-conjugated Goat Anti-Human IgG
NC : Negative control PC : Positive control
Specifications
- Host Species
- Human
- Type
- Human IgG1
- Specificity
- Human JAG1
- Species Reactivity
- Human, Mouse
- Applications
- ELISA, IF, Block
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term.
Target
Related Resources
TNF Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "JAG1"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-617 | Human Anti-JAG1 Recombinant Antibody; scFv Fragment | WB, ELISA, FuncS | Human scFv |
| HPAB-0966LY-S(P) | Human Anti-JAG1 Recombinant Antibody; scFv Fragment (HPAB-0966LY-S(P)) | ELISA | Human scfv |
| HPAB-0967LY-S(P) | Human Anti-JAG1 Recombinant Antibody; scFv Fragment (HPAB-0967LY-S(P)) | ELISA | Human scfv |
| HPAB-0968LY-S(P) | Human Anti-JAG1 Recombinant Antibody; scFv Fragment (HPAB-0968LY-S(P)) | ELISA | Human scfv |
| HPAB-0969LY-S(P) | Human Anti-JAG1 Recombinant Antibody; scFv Fragment (HPAB-0969LY-S(P)) | ELISA | Human scfv |
Customer Reviews and Q&As
Customer Review
Jenn***r
Excellent for IF
Excellent for IF
I've been on the hunt for a reliable antibody to study Notch signaling in endothelial cells. The Human Anti-JAG1 Recombinant Antibody has been a game-changer. In immunofluorescence (IF) experiments, after fixing and permeabilizing the cells, I incubated the antibody at room temperature for 1.5 hours. It beautifully stained JAG1, highlighting its cellular distribution and providing clear visual data crucial for my vascular biology research.
09-Oct-24
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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