Hi-Affi™ Recombinant Rabbit Anti-MAPK3 Monoclonal Antibody (TH173DS)
CAT#: MOR-4659
Recombinant Rabbit anti-MAPK3 monoclonal antibody for WB, IF, ICC, FCM, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2683/if_selected.jpg
Normal Tissue
Figure 2 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5700/20815_A_5_6.jpg
Normal Tissue
Figure 3 Breast
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Myoepithelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5700/21384_B_3_4.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5700/20815_A_9_8.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5700/20815_A_9_3.jpg
Normal Tissue
Figure 6 Liver
Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5700/20815_A_8_4.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5700/20815_A_9_5.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000102882-MAPK3
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Specifications
- Immunogen
- proprietary
- Host Species
- Rabbit
- Type
- IgG
- Species Reactivity
- Rat, Human
- Clone
- TH173DS
- Applications
- Used for immunoassay techniques such as: Western Blot; Immunofluorescence; Immunocytochemistry; Flow Cytometry
- Conjugate
- Unconjugated
Product Property
- Purification
- Purified with Absolute Mag™ Protein A Agarose
- Purity
- > 95% as determined by both RP-HPLC and SDS-PAGE analysis.
- Format
- Liquid
- Buffer
- PBS
- Preservative
- 0.09% sodium azide
- Storage
- Store at -20°C for long-term storage. Store at 4°C for up to one month. Avoid freeze/thaw cycles.
Applications
- Application Notes
- FC: 2-4 µg x 10^6 cells; ICC: 2-4 µg; IF: 2-4 µg; WB: 0.5-1 µg/ml
Target
- Alternative Names
- Mitogen-Activated Protein Kinase 3; Extracellular Signal-Regulated Kinase 1; Microtubule-Associated Protein 2 Kinase; Insulin-Stimulated MAP2 Kinase; MAP Kinase Isoform P44; EC 2.7.11.24; P44-ERK1; P44-MAPK; PRKM3; ERK-1; ERK1; ERT2; Extracellular Signal-Related Kinase 1; MAP Kinase 3; EC 2.7.11; HS44KDAP; HUMKER1A; P44ERK1; P44MAPK; MAPK 1; MAPK 3
- Gene ID
- 5595
- UniProt ID
- P27361
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Related Signaling Pathways
FcεR1 Signaling Pathway
TCR Signaling Pathway
Related Diseases
Maturity Onset Diabetes of the Young
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "MAPK3"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0342MZ | Chicken Anti-MAPK3 Polyclonal IgY | Indirect ELISA, WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2154 | Hi-Affi™ Recombinant Rabbit Anti-MAPK3 Monoclonal Antibody (DS2154AB) | WB, IP | IgG |
| MOR-4697 | Hi-Affi™ Recombinant Rabbit Anti-MAPK3 Monoclonal Antibody (TH211DS) | WB, IF, ICC, IHC-P, FC, ChIP, ELISA | IgG |
| MOR-0040-FY | Rabbit Anti-MAPK3 Recombinant Antibody (clone AFY0011) | IHC-P, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0543-CN | Recombinant Mouse Anti-MAPK3 Monoclonal Antibody (7-D6-E5) | WB, IF, IHC | Mouse IgG1 |
| MRO-0544-CN | Recombinant Rabbit Anti-MAPK3 Monoclonal Antibody (CBACN-215) | WB, IF, IHC | Rabbit IgG |
| MRO-0545-CN | Recombinant Rabbit Anti-MAPK3 Monoclonal Antibody (CBACN-216) | WB, IF, IHC, IP, FC | Rabbit IgG |
| MRO-1845-CN | Rabbit Anti-MAPK3 Polyclonal Antibody (MRO-1845-CN) | WB, IF, IHC | Rabbit IgG |
| ZG-0022C | Mouse Anti-MAPK3 Recombinant Antibody (clone 1C11) | IHC-P, IF | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY181 | AbPlus™ Anti-MAPK3 Magnetic Beads (CBACN-216) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
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| VS-1024-XY190 | Mouse Anti-NHP MAPK3 Recombinant Antibody (clone 12D11) | WB, IHC, IF | Mouse IgG |
| CAT | Product Name | Application | Type |
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| VS-0525-XY4236 | Anti-MAPK3 Immunohistochemistry Kit | IHC | |
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