Hi-Affi™ Recombinant Rabbit Anti-MAPK8 Monoclonal Antibody (TH149DS)
CAT#: MOR-4636
Recombinant Rabbit anti-MAPK8 monoclonal antibody for WB, IF, ICC, FCM, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
Gene Expression
Normal Tissue
Figure 1 IHC staining of human breast
Immunohistochemical staining of human breast shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4463/ihc_selected.jpg
Normal Tissue
Figure 2 IHC staining of human thyroid gland
Immunohistochemical staining of human thyroid gland shows moderate cytoplamsic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69396/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4463/12466_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4463/12962_A_7_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4463/12962_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4463/12962_A_4_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4463/12962_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000107643-MAPK8
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Specifications
- Immunogen
- Recombinant protein corresponding to amino acids 17-126 of human c-Jun N-terminal kinase 1
- Host Species
- Rabbit
- Type
- IgG
- Species Reactivity
- Human
- Clone
- TH149DS
- Applications
- Used for immunoassay techniques such as: Western Blot; Immunofluorescence; Immunocytochemistry; Flow Cytometry
- Conjugate
- Unconjugated
Product Property
- Purification
- Purified with Absolute Mag™ Protein A Agarose
- Purity
- > 95% as determined by both RP-HPLC and SDS-PAGE analysis.
- Format
- Liquid
- Buffer
- PBS
- Preservative
- 0.09% sodium azide
- Storage
- Store at -20°C for long-term storage. Store at 4°C for up to one month. Avoid freeze/thaw cycles.
Applications
- Application Notes
- FC: 3-5 µg/million cells; ICC: 1 µg/ml; IF: 1 µg/ml; WB: 1-3 µg/ml
Target
- Alternative Names
- Mitogen-Activated Protein Kinase 8; Stress-Activated Protein Kinase 1c; C-Jun N-Terminal Kinase 1; JUN N-Terminal Kinase; MAP Kinase 8; EC 2.7.11.24; JNK-46; SAPK1c; PRKM8; SAPK1; JNK1; Mitogen-Activated Protein Kinase 8 Isoform JNK1 Alpha1; Mitogen-Activated Protein Kinase 8 Isoform JNK1 Beta2; Stress-Activated Protein Kinase JNK1; Stress-Activated Protein Kinase 1; JNK21B1/2; EC 2.7.11; JNK1A2; MAPK 8; JNK
- Gene ID
- 5599
- UniProt ID
- P45983
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Related Signaling Pathways
FcεR1 Signaling Pathway
TCR Signaling Pathway
Related Diseases
Maturity Onset Diabetes of the Young
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "MAPK8"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1617z | Mouse Anti-MAPK8 Recombinant Antibody (clone 14A7) | WB, ELISA, IHC | Mouse IgG2a, κ |
| VS3-QX744 | Mouse Anti-MAPK8 Recombinant Antibody (clone 1A4-C5-F11) | WB, IF | Mouse IgG2a |
| VS3-QX745 | Mouse Anti-MAPK8 Recombinant Antibody (clone 1H8-E10-D2) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-FY890 | Recombinant Rabbit Anti-MAPK8 (phospho Thr183/Tyr185) Antibody (clone R02-0F1) | WB, IP | Rabbit IgG |
| VS3-FY891 | Recombinant Rabbit Anti-MAPK8 Antibody (clone R08-7H1) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2159 | Hi-Affi™ Recombinant Rabbit Anti-MAPK8 Monoclonal Antibody (DS2159AB) | WB | IgG |
| MOR-4691 | Hi-Affi™ Recombinant Rabbit Anti-MAPK8 Monoclonal Antibody (TH205DS) | WB, IF, ICC, IHC-P, FC, ChIP, ELISA | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC989 | AbPlus™ Anti-MAPK8 Magnetic Beads (VS-0724-YC989) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY292 | Mouse Anti-NHP MAPK8 Recombinant Antibody (clone 4A2G12) | WB, IF | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4244 | Anti-MAPK8 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4245 | Anti-Mouse MAPK8 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4246 | Anti-Rat MAPK8 Immunohistochemistry Kit | IHC |
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