Mouse Anti-NHP MAPK8 Recombinant Antibody (clone 4A2G12)
CAT#: VS-1024-XY292
Tailored for the specific recognition of MAPK8, this mouse monoclonal antibody demonstrates reactivity against human and non-human primate tissues. Developed using a recombinant GST-fusion protein of MAPK8 as the immunogen, it guarantees high specificity. Suitable for Western blot (WB) and immunofluorescence (IF), this antibody offers reliable performance in analyzing MAPK8 expression in various experimental frameworks.
Gene Expression
Normal Tissue
Figure 1 IHC staining of human breast
Immunohistochemical staining of human breast shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4463/ihc_selected.jpg
Normal Tissue
Figure 2 IHC staining of human thyroid gland
Immunohistochemical staining of human thyroid gland shows moderate cytoplamsic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69396/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4463/12466_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4463/12962_A_7_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4463/12962_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4463/12962_A_4_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4463/12962_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000107643-MAPK8
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Specifications
- Immunogen
- Recombinant GST-fusion protein of MAPK8
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- MAPK8
- Species Reactivity
- Human, Non-human primate
- Clone
- 4A2G12
- Applications
- WB, IF
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1-3 µg/mL, IF: 1-3 µg/mL
Target
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Related Signaling Pathways
FcεR1 Signaling Pathway
TCR Signaling Pathway
Related Diseases
Maturity Onset Diabetes of the Young
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Recommended Dilution Buffer
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "MAPK8"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1617z | Mouse Anti-MAPK8 Recombinant Antibody (clone 14A7) | WB, ELISA, IHC | Mouse IgG2a, κ |
| MRO-1987-CN | Rabbit Anti-MAPK8 Polyclonal Antibody (MRO-1987-CN) | WB, IF, IHC, FC | Rabbit IgG |
| ZG-0523J | Mouse Anti-MAPK8 Recombinant Antibody (ZG-0523J) | WB, ICC | Mouse IgG |
| VS-0923-FY201 | Recombinant Mouse Anti-MAPK8 Antibody (VS-0923-FY201) | ELISA, WB | Mouse IgG2b |
| VS3-FY2764 | Rabbit Anti-MAPK8 Recombinant Antibody (VS3-FY2764) | WB, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2159 | Hi-Affi™ Recombinant Rabbit Anti-MAPK8 Monoclonal Antibody (DS2159AB) | WB | IgG |
| MOR-4636 | Hi-Affi™ Recombinant Rabbit Anti-MAPK8 Monoclonal Antibody (TH149DS) | WB, IF, ICC, FC | IgG |
| MOR-4691 | Hi-Affi™ Recombinant Rabbit Anti-MAPK8 Monoclonal Antibody (TH205DS) | WB, IF, ICC, IHC-P, FC, ChIP, ELISA | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC989 | AbPlus™ Anti-MAPK8 Magnetic Beads (VS-0724-YC989) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4244 | Anti-MAPK8 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4245 | Anti-Mouse MAPK8 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4246 | Anti-Rat MAPK8 Immunohistochemistry Kit | IHC |
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