Anti-Human NOTCH2 Recombinant Antibody (TAB-H67) (CAT#: TAB-H67)
Recombinant human antibody to Human NOTCH2. It is a novel anti-cancer stem cell antibody identified by screening against the Notch3 receptor that binds to and prevents signaling through both the Notch2 and Notch3 receptors. It has been shown in preclinical models to have broad-spectrum anti-tumor activity via inhibition of cancer stem cell growth, and promoting cell differentiation, as well as disrupting tumor angiogenesis by inhibiting vascular pericytes.
Specific Inquiry
FC
Figure 1 Anti-Human NOTCH2 Recombinant Antibody (TAB-H67) in FC
Flow cytometry analysis of U87 MG cells with purified antibody TAB-H67 (QCAb249, Blue). Cells were blocked with Human TruStain FcXTM and washed with 0.5% BSA-PBS. Cells were then incubated with TAB-H67 (5 μl/5x10^5 cells) for 60 min at 4°C. The secondary antibody was FITC anti-human IgG antibody (3 μl) for 60 min at 4°C.
SDS-PAGE
Figure 2 Anti-Human NOTCH2 Recombinant Antibody (TAB-H67) in SDS-PAGE
SDS-PAGE analysis of TAB-H67 in reduced (Lane 1) and non-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result, different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
The purity of TAB-H67 was greater than 95% as determined by SDS-PAGE.
SEC-HPLC
Figure 3 Anti-Human NOTCH2 Recombinant Antibody (TAB-H67) in HPLC
The purity of TAB-H67 was greater than 95% as determined by SEC-HPLC.
WB
Figure 4 Anti-Human NOTCH2 Recombinant Antibody (TAB-H67) in WB
Western blot analysis of TAB-H67 was performed by loading Recombinant NOTCH2 protein.
TAB-H67 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-goat anti-human IgG
Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.3 μg)
Lane 3: Reducing Antigen (0.6 μg)
ELISA
Figure 5 Anti-Human NOTCH2 Recombinant Antibody (TAB-H67) in ELISA
ELISA analysis of TAB-H67 was performed by coating with Recombinant Human NOTCH2 Protein. Then blocked with BSA and incubated with Anti-Human NOTCH2 Recombinant Antibody (TAB-H67). The Goat Anti-Human IgG-HRP as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Specifications
- Host Species
- human
- Derivation
- human
- Type
- IgG2 - kappa
- Specificity
- NOTCH2 (notch 2) [Homo sapiens] ;
- Species Reactivity
- Human
- Applications
- FuncS, Block, ELISA
- Related Disease
- Pancreatic cancers
Product Property
- Purity
- >95%, by SDS-PAGE with silver staining, under reducing conditions.
Applications
- Application Notes
- The Human NOTCH2 antibody has been reported in applications of Functional Assay, Blocking, Enzyme-linked Immunosorbent Assay.
Target
- Alternative Names
- tarextumab;1359940-55-8;OMP-59R5;59M07;13M57;anti-Notch2/3;OMP-59R5;NOTCH2;notch 2;Notch (Drosophila) homolog 2 , Notch homolog 2 (Drosophila);neurogenic locus notch homolog protein 2;Notch homolog 2;hN2;AGS2;HJCYS;
- Gene ID
- 4853
- UniProt ID
- Q04721
Related Resources
Please refer to Tarextumab Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Tarextumab.
Breast Cancer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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Cancer Stem Cell Biomarker Specific Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2555z | Mouse Anti-NOTCH2 Recombinant Antibody (clone 19B5) | ELISA, IHC | Mouse IgG1, κ |
| HPAB-0595-WJ | Human Anti-NOTCH2 Recombinant Antibody (HPAB-0595-WJ) | ELISA, WB | Human IgG |
| HPAB-0596-WJ | Human Anti-NOTCH2 Recombinant Antibody (HPAB-0596-WJ) | ELISA, WB | Human IgG |
| VS3-FY1050 | Recombinant Mouse Anti-NOTCH2 Antibody (clone 2D1B2) | ELISA, IHC, ICC, FC | Mouse IgG2a |
| VS3-FY1051 | Recombinant Mouse Anti-NOTCH2 Antibody (clone 4C5A8) | ELISA, FC | Mouse IgG1 |
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To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-H67, RRID: AB_3112042)
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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