Rabbit Anti-NOTCH2 Antibody (VS-0924-YC80) - Cancer Stem Cell Marker
CAT#: VS-0924-YC80
Introducing our anti-NOTCH2 antibody (VS-0924-YC80) - specifically developed to target the human NOTCH2 protein, a well-known marker for cancer stem cells (CSCs). This antibody is ideal for detecting NOTCH2 in CSC tissues or cells. It can be utilized in Immunohistochemistry at dilutions of 1/200 to 1/500 as well as in Immunofluorescence at concentrations ranging from 0.5 to 2 µg/mL.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48743/714_F8_1_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48743/120061_B_7_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48743/120061_A_8_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48743/120061_A_7_5.jpg
Normal Tissue
Figure 5 Testis
Elongated or late spermatids Staining: Low Intensity: Moderate Quantity: <25% Sertoli cells Staining: Low Intensity: Moderate Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48743/120061_A_6_6.jpg
RNA Expression
Figure 6 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000134250-NOTCH2
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human NOTCH2
- Species Reactivity
- Human
- Applications
- IHC, IF
- Related Disease
- Disease variant, Osteoporosis
Product Property
- Clonality
- Polyclonal
- Purification
- Affinity purified using the PrEST antigen as affinity ligand
- Concentration
- 0.4 mg/mL
- Buffer
- PBS, pH 7.2, with 40% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Immunohistochemistry (IHC): 1/200-1/500
Immunofluorescence (IF): 0.5-2 µg/mL
Target
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Related Diseases
Breast Cancer
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "NOTCH2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2555z | Mouse Anti-NOTCH2 Recombinant Antibody (clone 19B5) | ELISA, IHC | Mouse IgG1, κ |
| HPAB-0595-WJ | Human Anti-NOTCH2 Recombinant Antibody (HPAB-0595-WJ) | ELISA, WB | Human IgG |
| HPAB-0596-WJ | Human Anti-NOTCH2 Recombinant Antibody (HPAB-0596-WJ) | ELISA, WB | Human IgG |
| FAMAB-1387CQ | Mouse Anti-NOTCH2 Recombinant Antibody (clone B9) | Inhib, Block, ELISA, IF | Mouse IgG1 |
| FAMAB-1389CQ | Mouse Anti-NOTCH2 Recombinant Antibody (clone B6) | Inhib, Block, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-H67 | Anti-Human NOTCH2 Recombinant Antibody (TAB-H67) | FuncS, Block, ELISA | IgG2 - kappa |
| TAB-552MZ | Human Anti-NOTCH2 Recombinant Antibody (TAB-552MZ) | ELISA | Human IgG |
| TAB-552MZ-S(P) | Human Anti-NOTCH2 Recombinant Antibody; scFv Fragment (TAB-552MZ-S(P)) | ELISA | Human scFv |
| TAB-552MZ-F(E) | Human Anti-NOTCH2 Recombinant Antibody; Fab Fragment (TAB-552MZ-F(E)) | ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0038-CN-S(P) | Human Anti-NOTCH2 Recombinant Antibody; scFv Fragment (HPAB-0038-CN-S(P)) | FC, ELISA | Human scFv |
| HPAB-0039-CN-S(P) | Human Anti-NOTCH2 Recombinant Antibody; scFv Fragment (HPAB-0039-CN-S(P)) | FC, ELISA | Human scFv |
| HPAB-0595-WJ-S(P) | Human Anti-NOTCH2 Recombinant Antibody scFv Fragment (HPAB-0595-WJ-S(P)) | ELISA, WB | Human scFv |
| HPAB-0596-WJ-S(P) | Human Anti-NOTCH2 Recombinant Antibody scFv Fragment (HPAB-0596-WJ-S(P)) | ELISA, WB | Human scFv |
| HPAB-0597-WJ-S(P) | Human Anti-NOTCH2 Recombinant Antibody scFv Fragment (HPAB-0597-WJ-S(P)) | ELISA, WB | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0038-CN-F(E) | Human Anti-NOTCH2 Recombinant Antibody; Fab Fragment (HPAB-0038-CN-F(E)) | FC, ELISA | Human Fab |
| HPAB-0039-CN-F(E) | Human Anti-NOTCH2 Recombinant Antibody; Fab Fragment (HPAB-0039-CN-F(E)) | FC, ELISA | Human Fab |
| HPAB-0595-WJ-F(E) | Human Anti-NOTCH2 Recombinant Antibody Fab Fragment (HPAB-0595-WJ-F(E)) | ELISA, WB | Human Fab |
| HPAB-0596-WJ-F(E) | Human Anti-NOTCH2 Recombinant Antibody Fab Fragment (HPAB-0596-WJ-F(E)) | ELISA, WB | Human Fab |
| HPAB-0597-WJ-F(E) | Human Anti-NOTCH2 Recombinant Antibody Fab Fragment (HPAB-0597-WJ-F(E)) | ELISA, WB | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-H67 | Afuco™ Anti-NOTCH2 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-H67) | IF, IP, Neut, FuncS, ELISA, FC | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC621 | AbPlus™ Anti-NOTCH2 Magnetic Beads (VS-0724-YC621) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY171 | CytoStream™ Rat Anti-NOTCH2 Recombinant Antibody (clone 16F11) | FC | Rat IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-FY160 | Human Anti-NOTCH2 scFv-Fc Chimera (VS-0425-FY160) | ELISA, Block, WB | Human IgG2, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1507 | Anti-NOTCH2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4909 | Anti-Mouse NOTCH2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4908 | Anti-Human NOTCH2 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC664 | Recombinant Anti-NOTCH2 Vesicular Antibody, EV Displayed (VS-0425-YC664) | ELISA, FC, Cell-uptake |
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