Human Anti-NOTCH2 scFv-Fc Chimera (VS-0425-FY160) (CAT#: VS-0425-FY160)
Similar to NOTCH1, NOTCH2 is another member of the NOTCH family of receptors with crucial roles in development and disease. It is involved in processes like B cell development and Notch signaling pathways. This scFv-FC can be used to target NOTCH2 for research or therapeutic purposes.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line MCF7
(Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48743/714_F8_1_selected.jpg
(Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48743/714_F8_1_selected.jpg
Figure 2 Cerebral cortex
(Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48743/120061_B_7_5.jpg
(Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48743/120061_B_7_5.jpg
Figure 3 Colon
(Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48743/120061_A_8_3.jpg
(Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48743/120061_A_8_3.jpg
Figure 4 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48743/120061_A_7_5.jpg
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48743/120061_A_7_5.jpg
Figure 5 Testis
(Elongated or late spermatids Staining: Low Intensity: Moderate Quantity: <25% Sertoli cells Staining: Low Intensity: Moderate Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48743/120061_A_6_6.jpg
(Elongated or late spermatids Staining: Low Intensity: Moderate Quantity: <25% Sertoli cells Staining: Low Intensity: Moderate Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/48743/120061_A_6_6.jpg
Figure 6 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000134250-NOTCH2
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000134250-NOTCH2
Specifications
- Host Species
- Human
- Type
- Human IgG2, scFv-Fc
- Specificity
- Human NOTCH2
- Species Reactivity
- Human
- Applications
- ELISA, Blocking, Western Blot
- Related Disease
- Alagille Syndrome 2 and Hajdu-Cheney Syndrome
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- The optimal dilution concentration is determined by the experiment.
Target
- Alternative Names
- Notch 2; HN2; Neurogenic Locus Notch Homolog Protein 2; Notch (Drosophila) Homolog 2; Notch Homolog 2 (Drosophila); Notch Homolog 2; HJCYS; AGS2
- Gene ID
- 4853
- UniProt ID
- Q04721
- Long Name
- Notch 2
- Sequence Similarities
- Belongs to the NOTCH family.
- Cellular Localization
- Cell membrane, Nucleus, Cytoplasm
- Post Translation Modifications
- Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form.
Hydroxylated by HIF1AN.
Can be either O-glucosylated or O-xylosylated at Ser-613 by POGLUT1.
Phosphorylated by GSK3
- Protein Refseq
- NP_001186930.1; NP_077719.2
- Function
- Functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination.
Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus.
Affects the implementation of differentiation, proliferation and apoptotic programs.
Involved in bone remodeling and homeostasis.
Related Resources
Breast Cancer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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Flow Cytometry (FC) related Reagents and Kits: Empowering Cell Function Research
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2555z | Mouse Anti-NOTCH2 Recombinant Antibody (clone 19B5) | ELISA, IHC | Mouse IgG1, κ |
| HPAB-0038-CN | Human Anti-NOTCH2 Recombinant Antibody (HPAB-0038-CN) | FC, ELISA | Human IgG2, κ |
| HPAB-0039-CN | Human Anti-NOTCH2 Recombinant Antibody (HPAB-0039-CN) | FC, ELISA | Human IgG2, κ |
| VS3-FY1050 | Recombinant Mouse Anti-NOTCH2 Antibody (clone 2D1B2) | ELISA, IHC, ICC, FC | Mouse IgG2a |
| VS3-FY1051 | Recombinant Mouse Anti-NOTCH2 Antibody (clone 4C5A8) | ELISA, FC | Mouse IgG1 |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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