CytoStream™ Rat Anti-NOTCH2 Recombinant Antibody (clone 16F11)
CAT#: VS-0225-XY171
Targeting NOTCH2, this antibody exhibits exceptional reactivity with mouse specimens. Optimized for flow cytometry applications, it allows precise characterization of NOTCH2 expression and its role in cellular signaling. This antibody is an invaluable tool for researchers studying developmental biology, stem cell regulation, and immune responses in mice, providing reliable and consistent results in flow cytometric analyses. Its specificity ensures robust detection of NOTCH2, making it an essential asset for exploring its functional implications.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48743/714_F8_1_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48743/120061_B_7_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48743/120061_A_8_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48743/120061_A_7_5.jpg
Normal Tissue
Figure 5 Testis
Elongated or late spermatids Staining: Low Intensity: Moderate Quantity: <25% Sertoli cells Staining: Low Intensity: Moderate Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/48743/120061_A_6_6.jpg
RNA Expression
Figure 6 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000134250-NOTCH2
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Specifications
- Immunogen
- Mouse Notch2 extracellular domain Recombinant Protein
- Host Species
- Rat
- Type
- Rat IgG1, kappa
- Specificity
- NOTCH2
- Species Reactivity
- Mouse
- Clone
- 16F11
- Applications
- FC
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS
- Preservative
- Sodium azide
- Storage
- Store at 4°C for short-term storage and at -20°C for long-term storage. Avoid repeated freeze-thaw cycles.
Target
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Related Diseases
Breast Cancer
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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Secondary Antibody
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-H67 | Anti-Human NOTCH2 Recombinant Antibody (TAB-H67) | FuncS, Block, ELISA | IgG2 - kappa |
| TAB-552MZ | Human Anti-NOTCH2 Recombinant Antibody (TAB-552MZ) | ELISA | Human IgG |
| TAB-552MZ-S(P) | Human Anti-NOTCH2 Recombinant Antibody; scFv Fragment (TAB-552MZ-S(P)) | ELISA | Human scFv |
| TAB-552MZ-F(E) | Human Anti-NOTCH2 Recombinant Antibody; Fab Fragment (TAB-552MZ-F(E)) | ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0038-CN-S(P) | Human Anti-NOTCH2 Recombinant Antibody; scFv Fragment (HPAB-0038-CN-S(P)) | FC, ELISA | Human scFv |
| HPAB-0039-CN-S(P) | Human Anti-NOTCH2 Recombinant Antibody; scFv Fragment (HPAB-0039-CN-S(P)) | FC, ELISA | Human scFv |
| HPAB-0595-WJ-S(P) | Human Anti-NOTCH2 Recombinant Antibody scFv Fragment (HPAB-0595-WJ-S(P)) | ELISA, WB | Human scFv |
| HPAB-0596-WJ-S(P) | Human Anti-NOTCH2 Recombinant Antibody scFv Fragment (HPAB-0596-WJ-S(P)) | ELISA, WB | Human scFv |
| HPAB-0597-WJ-S(P) | Human Anti-NOTCH2 Recombinant Antibody scFv Fragment (HPAB-0597-WJ-S(P)) | ELISA, WB | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0038-CN-F(E) | Human Anti-NOTCH2 Recombinant Antibody; Fab Fragment (HPAB-0038-CN-F(E)) | FC, ELISA | Human Fab |
| HPAB-0039-CN-F(E) | Human Anti-NOTCH2 Recombinant Antibody; Fab Fragment (HPAB-0039-CN-F(E)) | FC, ELISA | Human Fab |
| HPAB-0595-WJ-F(E) | Human Anti-NOTCH2 Recombinant Antibody Fab Fragment (HPAB-0595-WJ-F(E)) | ELISA, WB | Human Fab |
| HPAB-0596-WJ-F(E) | Human Anti-NOTCH2 Recombinant Antibody Fab Fragment (HPAB-0596-WJ-F(E)) | ELISA, WB | Human Fab |
| HPAB-0597-WJ-F(E) | Human Anti-NOTCH2 Recombinant Antibody Fab Fragment (HPAB-0597-WJ-F(E)) | ELISA, WB | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-H67 | Afuco™ Anti-NOTCH2 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-H67) | IF, IP, Neut, FuncS, ELISA, FC | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0595-WJ | Human Anti-NOTCH2 Recombinant Antibody (HPAB-0595-WJ) | ELISA, WB | Human IgG |
| HPAB-0596-WJ | Human Anti-NOTCH2 Recombinant Antibody (HPAB-0596-WJ) | ELISA, WB | Human IgG |
| HPAB-0597-WJ | Human Anti-NOTCH2 Recombinant Antibody (HPAB-0597-WJ) | ELISA, WB | Human IgG |
| FAMAB-1387CQ | Mouse Anti-NOTCH2 Recombinant Antibody (clone B9) | Inhib, Block, ELISA, IF | Mouse IgG1 |
| FAMAB-1389CQ | Mouse Anti-NOTCH2 Recombinant Antibody (clone B6) | Inhib, Block, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC621 | AbPlus™ Anti-NOTCH2 Magnetic Beads (VS-0724-YC621) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0924-YC80 | Rabbit Anti-NOTCH2 Antibody (VS-0924-YC80) - Cancer Stem Cell Marker | IHC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-FY160 | Human Anti-NOTCH2 scFv-Fc Chimera (VS-0425-FY160) | ELISA, Block, WB | Human IgG2, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1507 | Anti-NOTCH2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4909 | Anti-Mouse NOTCH2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4908 | Anti-Human NOTCH2 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC664 | Recombinant Anti-NOTCH2 Vesicular Antibody, EV Displayed (VS-0425-YC664) | ELISA, FC, Cell-uptake |
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