Anti-Nectin-4 (Enfortumab)-mc-val-cit-PABC-MMAE ADC (AGS-22ME)
CAT#: ADC-L004
This ADC product is composed of an anti-Nectin-4 antibody (Enfortumab) conjuagated via a mc-val-cit-PABC linker to MMAE (Enfortumab-mc-val-cit-PABC-MMAE). It has demonstrated a response in solid tumor treatment by a MOA (Mechanism of Action) of Depolymerize Microtubules.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10775/1671_G1_2_selected.jpg
Normal Tissue
Figure 2 Skin 1
High expression in skin 1
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10775/27075_B_9_1_val_selected.jpg
Normal Tissue
Figure 3 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10775/27075_A_9_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10775/27075_A_9_5.jpg
Normal Tissue
Figure 5 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10775/27075_A_6_6.jpg
Normal Tissue
Figure 6 Skin
Epidermal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10775/27075_B_5_8.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (BEWO, HaCaT, MCF7, OE19, SK-BR-3, T-47d)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000143217-NECTIN4
❮
❯
Specifications
- Antibody Overview
- Fully human IgG1k monoclonal antibody that binds to Nectin-4
- Clone
- Enfortumab
- Antibody Conjugation
- Human
- Linker
- mc-val-cit-PABC (cleavable maleimidocaproyl-valyl-citrullinyl-p-aminobenzyloxycarbonyl)
- Linker Class/Description
- Class: Enzymatically cleavable linkers-Peptide linkers
Description: peptide linkers, belonging to Enzymatically cleavable linkers, combine greater systemic stability with rapid enzymatic release of the drug in the target cell. The scission of peptidic bonds relies on lysosomal proteolytic enzymes, which have very low activities in blood due to endogenous inhibitors and the unfavorably high pH value of blood.
- Drug
- MMAE (Monomethyl auristatin E)
- Drug Class/Description
- Auristatins are water-soluble dolastatin analogs of dolastatin 10. Dolastatin 10 belongs to dolastatin family and it can powerfully bind to tubulin, thus inhibiting polymerization mediated through the binding to the vinca alkaloid binding domain, and causes cell to accumulate in metaphase arrest.
Target
- Introduction
- This gene encodes a member of the nectin family. The encoded protein contains two immunoglobulin-like (Ig-like) C2-type domains and one Ig-like V-type domain. It is involved in cell adhesion through trans-homophilic and -heterophilic interactions. It is a single-pass type I membrane protein. The soluble form is produced by proteolytic cleavage at the cell surface by the metalloproteinase ADAM17/TACE. The secreted form is found in both breast tumor cell lines and breast tumor patients. Mutations in this gene are the cause of ectodermal dysplasia-syndactyly syndrome type 1, an autosomal recessive disorder. Alternatively spliced transcript variants have been found but the full-length nature of the variant has not been determined.
- Alternative Names
- LNIR; PRR4; EDSS1; PVRL4; nectin-4
- Gene ID
- 81607
- UniProt ID
- Q96NY8
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for ADC-L004. Click the button above to contact us or submit your feedback about this product.
Effective Cleavable Linker Design
We acquired this antibody-drug conjugate to investigate linker stability in our biochemical assays. The mc-val-cit-PABC linker performed exactly as described in the product specifications. It remained stable during handling but released the payload efficiently when exposed to the appropriate enzymes in our lysosomal simulation. This product provided the reliable data we needed for our comparative study.
Strong Specificity For Nectin-4
I used this product as a benchmark in my research on Nectin-4 expression. The Enfortumab component demonstrated high binding affinity to the target protein on our cell lines. The conjugation with MMAE was consistent, and we observed clear results in our cellular internalization experiments. It is a high-quality reagent for anyone studying this specific antigen pathway.
Q&As
-
What specific payload and linker technology does this antibody-drug conjugate utilize?
A: This product is conjugated with the cytotoxic agent Monomethyl auristatin E, commonly known as MMAE. We attach this payload to the antibody using a protease-cleavable linker composed of a valine-citrulline dipeptide (mc-val-cit-PABC). This design allows for the targeted release of the drug after the molecule enters the cell.
-
How does the mechanism of action work for this product after it binds to the target?
A: Once the Enfortumab antibody binds to Nectin-4 on the cell surface, the entire complex is internalized by the cell. Inside the lysosome, enzymes cleave the linker. This releases free MMAE into the cell, where it binds to tubulin and inhibits polymerization, which disrupts the cellular structure.
View the frequently asked questions answered by Creative Biolabs Support.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Clone Enfortumab"
- CAT
- Product Name
See other products for "NECTIN4"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-H25 | Human Anti-NECTIN4 Recombinant Antibody (TAB-H25) | ELISA, Neut, IF, IP, FC, FuncS | Human IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0034-DJB | Mouse Anti-NECTIN4 Recombinant Antibody (clone N41) | ELISA, IHC, FC | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0034-DJB-S(P) | Mouse Anti-NECTIN4 Recombinant Antibody (clone N41); scFv Fragment | ELISA, IHC, FC | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0034-DJB-F(E) | Mouse Anti-NECTIN4 Recombinant Antibody (clone N41); Fab Fragment | ELISA, IHC, FC | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS4-CJ51 | Recombinant Human Anti-NECTIN4 Antibody (clone 6D3) | ELISA | Human IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0723-WK124 | Human Anti-NECTIN4 Recombinant Antibody (VS-0723-WK124) | FC | Chimeric (rabbit/human) IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY114 | Human Anti-NECTIN4 (clone Enfortumab) scFv-Fc Chimera | ELISA, Neut, IF, IP, FC | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC71 | Recombinant Anti-NECTIN4 Vesicular Antibody, EV Displayed (VS-0425-YC71) | ELISA, FC, Neut, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0825-YC265 | SmartAb™ Recombinant Anti-NECTIN4 pH-dependent Antibody (VS-0825-YC265) | ELISA, Neut, IF, IP, FC | Human IgG1 kappa |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: WB, ELISA, IP, FC, FuncS, Neut, IF
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: WB, ELISA, FC, IP, FuncS, IF, Neut
Application: FC, IP, ELISA, Neut, FuncS, IF, WB
Application: WB, ELISA, IP, FC, FuncS, Neut, IF
Application: Neut, ELISA, IF, IP, FuncS, FC, IHC
Application: WB, FC, IP, ELISA, Neut, FuncS, IF
Application: WB, FC, IP, ELISA, Neut, FuncS, IF
Application: WB, Neut, ELISA, IF, IP, FuncS, FC
Application: ELISA, FC, IP, FuncS, IF, Neut, WB
Application: ELISA, IP, WB, IHC, IF, FuncS
Application: FuncS, Inhib, IP, ELISA
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
