Mouse Anti-NECTIN4 Recombinant Antibody (NE35)
CAT#: VS-1225-YC734
The Mouse Anti-NECTIN4 Recombinant Antibody (NE35) is a mouse monoclonal antibody against human NECTIN4 for IHC staining. NECTIN4 is an Ig-like cell adhesion molecule that participates in the formation of adherens junctions. It promotes tumor cell proliferation and survival by activating the PI3K/AKT pathway.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10775/1671_G1_2_selected.jpg
Normal Tissue
Figure 2 Skin 1
High expression in skin 1
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10775/27075_B_9_1_val_selected.jpg
Normal Tissue
Figure 3 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10775/27075_A_9_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10775/27075_A_9_5.jpg
Normal Tissue
Figure 5 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10775/27075_A_6_6.jpg
Normal Tissue
Figure 6 Skin
Epidermal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10775/27075_B_5_8.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (BEWO, HaCaT, MCF7, OE19, SK-BR-3, T-47d)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000143217-NECTIN4
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- NECTIN4
- Species Reactivity
- Human
- Clone
- NE35
- Applications
- Immunohistochemistry (IHC)
- Related Disease
- Cancer
Product Property
- Clonality
- Monoclonal
- Format
- Liquid
- Storage
- Store at 4˚C short term. Store at -20˚C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- Immunohistochemistry (IHC): 1/500
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "NECTIN4"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-H25 | Human Anti-NECTIN4 Recombinant Antibody (TAB-H25) | ELISA, Neut, IF, IP, FC, FuncS | Human IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0034-DJB | Mouse Anti-NECTIN4 Recombinant Antibody (clone N41) | ELISA, IHC, FC | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0034-DJB-S(P) | Mouse Anti-NECTIN4 Recombinant Antibody (clone N41); scFv Fragment | ELISA, IHC, FC | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0034-DJB-F(E) | Mouse Anti-NECTIN4 Recombinant Antibody (clone N41); Fab Fragment | ELISA, IHC, FC | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS4-CJ51 | Recombinant Human Anti-NECTIN4 Antibody (clone 6D3) | ELISA | Human IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0723-WK124 | Human Anti-NECTIN4 Recombinant Antibody (VS-0723-WK124) | FC | Chimeric (rabbit/human) IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY114 | Human Anti-NECTIN4 (clone Enfortumab) scFv-Fc Chimera | ELISA, Neut, IF, IP, FC | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC71 | Recombinant Anti-NECTIN4 Vesicular Antibody, EV Displayed (VS-0425-YC71) | ELISA, FC, Neut, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0825-YC265 | SmartAb™ Recombinant Anti-NECTIN4 pH-dependent Antibody (VS-0825-YC265) | ELISA, Neut, IF, IP, FC | Human IgG1 kappa |
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