Hi-Affi™ Recombinant Rabbit Anti-PIK3CA Monoclonal Antibody (DS2707AB)
CAT#: MOR-2707
Recombinant Rabbit anti-PIK3CA monoclonal antibody for ELISA, IHC-P, WB, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9985/68_B8_2_selected.jpg
Normal Tissue
Figure 2 IHC staining of human adrenal gland
Immunohistochemical staining of human adrenal gland shows strong cytoplasmic positivity in cortical cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9985/ihc_selected.jpg
Normal Tissue
Figure 3 IHC staining of human appendix
Immunohistochemical staining of human appendix shows cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17804/ihc_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9985/24769_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9985/24713_A_9_3.jpg
Normal Tissue
Figure 6 Liver
Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9985/24713_A_9_4.jpg
Normal Tissue
Figure 7 Kidney
Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9985/24713_A_9_5.jpg
Normal Tissue
Figure 8 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9985/24713_A_6_6.jpg
Normal Tissue
Figure 9 Lymph node
Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9985/24713_A_9_8.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000121879-PIK3CA
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Specifications
- Immunogen
- Synthetic peptide (Human) from the the center region of PI 3 Kinase catalytic subunit alpha conjugated to KLH.
- Host Species
- Rabbit
- Derivation
- Phage display library screening
- Type
- IgG
- Species Reactivity
- Human
- Clone
- DS2707AB
- Applications
- Used for immunoassay techniques such as: Enzyme-linked Immunosorbent Assay; Immunohistochemistry (Paraffin); Western Blot
- Conjugate
- Unconjugated
Product Property
- Purification
- Purified with Absolute Mag™ Protein A Agarose
- Purity
- > 95% as determined by both RP-HPLC and SDS-PAGE analysis.
- Format
- Liquid
- Storage
- Store at -20°C for long-term storage. Store at 4°C for up to one month. Avoid freeze/thaw cycles.
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "PIK3CA"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1459z | Mouse Anti-PIK3CA Recombinant Antibody (clone 20G7) | WB, IP, IF, FC, ELISA | Mouse IgG1, κ |
| ZG-0511U | Rabbit Anti-PIK3CA Recombinant Antibody (clone 10E5) | ELISA, WB, IF | Rabbit IgG |
| VS3-FY1138 | Recombinant Mouse Anti-PIK3CA Antibody (clone 3C1-6F9-6C4) | IHC-P | Mouse IgG1 |
| VS13-YC901 | Rabbit Anti-PIK3CA Recombinant Antibody (VS13-YC901) | WB, ICC, IF, IP | Rabbit IgG |
| VS7-0425-WR612 | Mouse Anti-PIK3CA Monoclonal Antibody (clone 7H9B3 ) | IHC, ICC, FC | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-G056E | Anti-PIK3CA immunotoxin (scFv)-PE | Cytotoxicity assay, Function study |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1650 | Anti-PIK3CA Immunohistochemistry Kit | IHC | |
| VS-0525-XY5405 | Anti-Mouse PIK3CA Immunohistochemistry Kit | IHC | |
| VS-0525-XY5404 | Anti-Human PIK3CA Immunohistochemistry Kit | IHC |
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