Mouse Anti-PIK3CA Recombinant Antibody (clone 20G7)
CAT#: MOB-1459z
This product is a mouse antibody that recognizes human PIK3CA. It can be used in the following applications: WB, IP, IF, FCM, ELISA.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9985/68_B8_2_selected.jpg
Normal Tissue
Figure 2 IHC staining of human adrenal gland
Immunohistochemical staining of human adrenal gland shows strong cytoplasmic positivity in cortical cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9985/ihc_selected.jpg
Normal Tissue
Figure 3 IHC staining of human appendix
Immunohistochemical staining of human appendix shows cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17804/ihc_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9985/24769_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9985/24713_A_9_3.jpg
Normal Tissue
Figure 6 Liver
Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9985/24713_A_9_4.jpg
Normal Tissue
Figure 7 Kidney
Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9985/24713_A_9_5.jpg
Normal Tissue
Figure 8 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9985/24713_A_6_6.jpg
Normal Tissue
Figure 9 Lymph node
Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9985/24713_A_9_8.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000121879-PIK3CA
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Specifications
- Immunogen
- Human Phosphatidylinositol-4,5-Bisphosphate 3-Kinase, Catalytic Subunit Alpha
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG1, κ
- Antibody Isotype
- IgG1, κ
- Specificity
- Human PIK3CA
- Species Reactivity
- Human
- Clone
- 20G7
- Applications
- WB, IP, IF, FC, ELISA
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Western blot, Immunoprecipitation, Immunofluorescence, Flow Cytometry, Enzyme-linked Immunosorbent Assay.
Target
- Alternative Names
- PIK3CA; PtdIns-3-Kinase Subunit P110-Alpha; Phosphoinositide-3-Kinase, Catalytic, Alpha Polypeptide; CLOVE; Phosphatidylinositol-4,5-Bisphosphate 3-Kinase, Catalytic Subunit Alpha; PI3K-Alpha; Phosphoinositide-3-Kinase Catalytic Alpha Polypeptide; PI3-Kin
- UniProt ID
- P42336
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Effective For Immunoprecipitation
Clone 20G7 worked very well for immunoprecipitation of PIK3CA from cell lysates. We were able to pull down the protein efficiently for downstream analysis. The antibody is highly specific to the human target and produced clean results in our subsequent Western blots.
Specific To Human PIK3CA
This mouse recombinant antibody demonstrated high specificity for human PIK3CA in our flow cytometry experiments. It allowed us to distinguish expression levels across different cell lines accurately. The product documentation was accurate regarding its diverse applications including IF and ELISA.
Q&As
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Can this antibody be used for Flow Cytometry?
A: Yes, this mouse recombinant antibody (clone 20G7) has been reported for use in Flow Cytometry (FC). It is also validated for Western Blot, Immunoprecipitation (IP), Immunofluorescence (IF), and Enzyme-linked Immunosorbent Assay (ELISA).
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What is the isotype of this antibody?
A: The isotype of this recombinant antibody is Mouse IgG1, kappa. It is a monoclonal antibody designed to recognize human PIK3CA and is suitable for various immunological research applications.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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