Recombinant Rabbit Anti-PIK3CA Monoclonal Antibody (SJ0186)
CAT#: MRO-1221-CN
This antibody is a rabbit monoclonal antibody that is capable of binding to PIK3CA.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9985/68_B8_2_selected.jpg
Normal Tissue
Figure 2 IHC staining of human adrenal gland
Immunohistochemical staining of human adrenal gland shows strong cytoplasmic positivity in cortical cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9985/ihc_selected.jpg
Normal Tissue
Figure 3 IHC staining of human appendix
Immunohistochemical staining of human appendix shows cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17804/ihc_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9985/24769_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9985/24713_A_9_3.jpg
Normal Tissue
Figure 6 Liver
Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9985/24713_A_9_4.jpg
Normal Tissue
Figure 7 Kidney
Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9985/24713_A_9_5.jpg
Normal Tissue
Figure 8 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9985/24713_A_6_6.jpg
Normal Tissue
Figure 9 Lymph node
Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9985/24713_A_9_8.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000121879-PIK3CA
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Specifications
- Immunogen
- Human PI 3 Kinase catalytic subunit alpha aa 1000 to the C-terminus
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human
- Species Reactivity
- Human, Mouse
- Clone
- SJ0186
- Applications
- Used for immunoassay techniques such as: Western Blot; Immunofluorescence; Immunoprecipitation
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A affinity purified
- Format
- Liquid
- Buffer
- 1*TBS, pH 7.4, 1% BSA, 40% Glycerol
- Preservative
- 0.05% Sodium Azide
- Storage
- Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Target
- Alternative Names
- Phosphatidylinositol-4,5-Bisphosphate 3-Kinase Catalytic Subunit Alpha; Phosphoinositide-3-Kinase, Catalytic, Alpha Polypeptide; Serine/Threonine Protein Kinase PIK3CA; PtdIns-3-Kinase Subunit P110-Alpha; PI3K-Alpha; Phosphatidylinositol-4,5-Bisphosphate 3-Kinase Catalytic Subunit, Alpha Isoform; Phosphatidylinositol 4,5-Bisphosphate 3-Kinase Catalytic Subunit Alpha Isoform; Phosphatidylinositol-4,5-Bisphosphate 3-Kinase 110 KDa Catalytic Subunit Alpha; Phosphatidylinositol 4,5-Bisphosphate 3-Kinase 110 KDa Catalytic Subunit Alpha; Phosphatidylinositol-4,5-Bisphosphate 3-Kinase, Catalytic Subunit Alpha; Phosphatidylinositol 3-Kinase, Catalytic, Alpha Polypeptide; Phosphatidylinositol 3-Kinase, Catalytic, 110-KD, Alpha; Phosphoinositide-3-Kinase Catalytic Alpha Polypeptide; PI3-Kinase P110 Subunit Alpha; PtdIns-3-Kinase Subunit Alpha;
- Gene ID
- 5290
- UniProt ID
- P42336
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "PIK3CA"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1459z | Mouse Anti-PIK3CA Recombinant Antibody (clone 20G7) | WB, IP, IF, FC, ELISA | Mouse IgG1, κ |
| VS3-QX871 | Mouse Anti-PIK3CA Recombinant Antibody (clone 4F3) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-CJ951 | Mouse Anti-PIK3CA Recombinant Antibody (clone ABT-PI3.H1047R) | WB, IF, IHC-P | Mouse IgG3, κ |
| VS3-CJ952 | Mouse Anti-PIK3CA Recombinant Antibody (clone PT0828) | WB, IHC-P | Mouse IgG1, κ |
| VS3-FY1138 | Recombinant Mouse Anti-PIK3CA Antibody (clone 3C1-6F9-6C4) | IHC-P | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-G056E | Anti-PIK3CA immunotoxin (scFv)-PE | Cytotoxicity assay, Function study |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2707 | Hi-Affi™ Recombinant Rabbit Anti-PIK3CA Monoclonal Antibody (DS2707AB) | ELISA, IHC-P, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1650 | Anti-PIK3CA Immunohistochemistry Kit | IHC | |
| VS-0525-XY5405 | Anti-Mouse PIK3CA Immunohistochemistry Kit | IHC | |
| VS-0525-XY5404 | Anti-Human PIK3CA Immunohistochemistry Kit | IHC |
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