Anti-Human PTN Recombinant Antibody (3B10)
CAT#: TAB-0333CL
The antibody is an anti-PTN antibody specifically binds to pleiotrophin which is highly expressed during development but with very specific and limited expression in normal adult tissue. By neutralizing one biological activity of PTN and inhibit cancer cell growth and angiogenesis, the PTN-specific antibody can be used in the treatment of cancers such as pancreatic cancer, glioblastoma, choriocarcinoma and breast cancer.
Published Data
Gene Expression
IHC
Figure 1 PTN protein expression in mammary glands and mammary epithelial cells (MECs).
Immunohistochemistry of virgin mouse mammary glands with a polyclonal anti-PTN antibody or secondary antibody only (sec. only) as a negative control.
Rosenfield, S. M., Bowden, E. T., Cohen-Missner, S., Gibby, K. A., Ory, V., Henke, R. T., ... & Wellstein, A. (2012). Pleiotrophin (PTN) expression and function and in the mouse mammary gland and mammary epithelial cells. PloS one, 7(10), e47876.
IF
Figure 2 PTN protein expression and function in mammary epithelial cells (MECs).
Immunofluorescence (IF) staining of MECs grown on poly-D-lysine coated slides. PTN (green), DAPI (blue), Phalloidin (red). Images are shown as merged images of anti-PTN with DAPI and anti-PTN with Phalloidin staining.
Rosenfield, S. M., Bowden, E. T., Cohen-Missner, S., Gibby, K. A., Ory, V., Henke, R. T., ... & Wellstein, A. (2012). Pleiotrophin (PTN) expression and function and in the mouse mammary gland and mammary epithelial cells. PloS one, 7(10), e47876.
IF
Figure 3 PTN effect on mammosphere formation.
Time course of mammosphere formation day 1, 3 and 5 without and with a blocking anti-PTN antibody ± recombinant PTN protein. Representative mammospheres that meet the threshold surface area of>900 µm² are indicated by an arrowhead.
Rosenfield, S. M., Bowden, E. T., Cohen-Missner, S., Gibby, K. A., Ory, V., Henke, R. T., ... & Wellstein, A. (2012). Pleiotrophin (PTN) expression and function and in the mouse mammary gland and mammary epithelial cells. PloS one, 7(10), e47876.
Block
Figure 4 PTN effect on MAP kinase pathway activity in developing mammary glands.
Representative Western blots of whole cell lysates from MECs treated for 24 hours with either UO126 (500 nM) alone or in combination with anti-PTN blocking antibody and quantitation of phospho ERK1/2 relative to total ERK1/2.
Rosenfield, S. M., Bowden, E. T., Cohen-Missner, S., Gibby, K. A., Ory, V., Henke, R. T., ... & Wellstein, A. (2012). Pleiotrophin (PTN) expression and function and in the mouse mammary gland and mammary epithelial cells. PloS one, 7(10), e47876.
Block
Figure 5 PTN effect on cultured mammary epithelial cell (MEC) differentiation.
Effect of treatment of MECs with a blocking anti-PTN antibody.
Rosenfield, S. M., Bowden, E. T., Cohen-Missner, S., Gibby, K. A., Ory, V., Henke, R. T., ... & Wellstein, A. (2012). Pleiotrophin (PTN) expression and function and in the mouse mammary gland and mammary epithelial cells. PloS one, 7(10), e47876.
ELISA
Figure 6 Anti-PTN antibody binding activity to PTN and its detection from mouse serum of treated mice.
Anti-PTN antibody binding to immobilized PTN by ELISA. Anti-PTN antibody KD-value = 1 nM. Control = antibody elution buffer. OD defines absorbance at 450 nm.
Rosenfield, S. M., Bowden, E. T., Cohen-Missner, S., Gibby, K. A., Ory, V., Henke, R. T., ... & Wellstein, A. (2012). Pleiotrophin (PTN) expression and function and in the mouse mammary gland and mammary epithelial cells. PloS one, 7(10), e47876.
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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line AF22
Immunofluorescent staining of human cell line AF22 shows localization to endoplasmic reticulum.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/73913/1783_H10_4_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Neuronal cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17685/164389_B_8_5.jpg
Normal Tissue
Figure 3 Testis
Pachytene spermatocytes
Staining: High
Intensity: Strong
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17685/164396_A_6_6.jpg
RNA Expression
Figure 4 RNA cell line category: Cell line enriched (AF22)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000105894-PTN/subcellular
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Specifications
- Host Species
- Mouse
- Specificity
- Human
- Clone
- 3B10
- Applications
- IHC, IF, Block, ELISA
- Related Disease
- Pancreatic cancer, glioblastoma, choriocarcinoma, breast cancer
Applications
- Application Notes
- The PTN antibody has been reported in applications of Immunohistochemistry, Immunofluorescence, Blocking, Enzyme-linked Immunosorbent Assay.
IHC: The primary goat anti-human PTN antibody diluted 1∶500 was incubated at 4°C overnight. The slides were counterstained with hematoxylin. Tissue sections are representative of 3–4 mice. Image capturing for stained section was performed with a Nikon E600 Epifluorescence Microscope.
IF: 1∶100
Target
- Alternative Names
- PTN; pleiotrophin; HARP; HBNF; HBGF8; NEGF1; HBBM; OSF-1; HB-GAM; HBGF-8; HBNF-1; osteoblast-specific factor 1; heparin-binding brain mitogen; heparin binding growth factor 8; heparin-binding growth factor 8; heparin affin regulatory protein; neurite growth-promoting factor 1; heparin-binding growth-associated molecule; heparin-binding neurite outgrowth-promoting factor 1; pleiotrophin (heparin binding growth factor 8, neurite growth-promoting factor 1)
- Gene ID
- 5764
- UniProt ID
- A0A024R778
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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COA
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2000z | Mouse Anti-PTN Recombinant Antibody (clone 39C3) | WB, FC, IF, IHC, IP | Mouse IgG1, κ |
| MOB-1271CT | Recombinant Mouse anti-Human PTN Monoclonal antibody (EML653) | IHC-P, WB | |
| VS3-FY1223 | Recombinant Rabbit Anti-PTN Antibody (clone R01-2J3) | WB, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0333CL-S(P) | Anti-Human PTN Recombinant Antibody scFv Fragment (3B10) | FuncS, Inhib | |
| TAB-290CT | Anti-Human PTN Recombinant Antibody (4B2) | WB, ELISA, IHC, Neut | |
| TAB-291CT | Anti-Human PTN Recombinant Antibody (3-4A) | WB, ELISA, IHC, Neut | |
| TAB-290CT-F(E) | Anti-Human PTN Recombinant Antibody Fab Fragment (4B2) | WB, ELISA, IHC, Neut | |
| TAB-291CT-F(E) | Anti-Human PTN Recombinant Antibody Fab Fragment (3-4A) | WB, ELISA, IHC, Neut |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2926 | Hi-Affi™ Recombinant Rabbit Anti-PTN Monoclonal Antibody (DS2926AB) | WB, IP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5853 | Anti-PTN Immunohistochemistry Kit | IHC | |
| VS-0525-XY5854 | Anti-Rat PTN Immunohistochemistry Kit | IHC | |
| VS-0525-XY5855 | Anti-Mouse PTN Immunohistochemistry Kit | IHC |
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