Mouse Anti-S100A4 Recombinant Antibody (TAB-0136CL)
CAT#: TAB-0136CL
This product is a recombinant mouse antibody that recognizes S100A4. The antibody was expressed in mammalian cells with chemically defined culture media and was purified by affinity chromatography.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A549
Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7486/2064_B12_5_selected.jpg
Normal Tissue
Figure 2 Lung.
High expression in lung
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68227/152283_A_1_4_val_selected.jpg
Normal Tissue
Figure 3 Spleen
High expression in spleen
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7973/106662_B_7_4_val_selected.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7973/106662_A_7_3.jpg
Normal Tissue
Figure 5 Testis
Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7973/106662_A_6_6.jpg
Normal Tissue
Figure 6 Placenta
Decidual cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Trophoblastic cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7973/106662_A_2_7.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27387/106652_A_9_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (A549, ASC diff, BJ hTERT+, fHDF/TERT166, RPMI-8226)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000196154-S100A4
❮
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Mouse S100A4
- Species Reactivity
- Human, Mouse
- Applications
- WB, IF, Block
- Related Disease
- Various cancers, such as gastric cancer, pancreatic cancer, colorectal cancer, thyroid cancer, breast cancer, squamous cell carcinoma, non-small cell lung cancer, prostate cancer, lung cancer, head and neck cancer, brain cancer (including glioblastoma multiforme), renal cell carcinoma; inflammatory diseases
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- S100A4; S100 calcium binding protein A4; 42A; 18A2; CAPL; FSP1; MTS1; P9KA; PEL98; protein S100-A4; protein Mts1; fibroblast-specific protein-1; placental calcium-binding protein; malignant transformation suppression 1; leukemia multidrug resistance associated protein; S100 calcium-binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog)
- Gene ID
- 6275
- UniProt ID
- P26447
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Excellent For Calcium Binding Proteins
We used this recombinant mouse antibody to study S100A4 expression in various cancer cell lines. It performed well in Western Blot and Immunofluorescence, giving clear signals. It helped us investigate the role of this calcium-binding protein in cell motility and metastasis in our in vitro models.
Q&As
-
Does this antibody react with both human and mouse S100A4?
A: Yes, this recombinant mouse antibody recognizes S100A4 from both Human and Mouse species. It is expressed in mammalian cells and is suitable for cross-species research.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "S100A4"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3568z | Mouse Anti-S100A4 Recombinant Antibody (clone 24D3) | WB, IP, IF, IHC, ELISA | Mouse IgG2a, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-546CL | Human Anti-S100A4 Recombinant Antibody (TAB-546CL) | ELISA | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0135CL-S(P) | Mouse Anti-S100A4 Recombinant Antibody; scFv Fragment (TAB-0135CL-S(P)) | WB, ELISA, BI, Block, Inhib | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0136CL-S(P) | Mouse Anti-S100A4 Recombinant Antibody; scFv Fragment (TAB-0136CL-S(P)) | WB, ELISA, BI, Block, Inhib | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0135CL-F(E) | Mouse Anti-S100A4 Recombinant Antibody; Fab Fragment (TAB-0135CL-F(E)) | WB, ELISA, BI, Block, Inhib | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0136CL-F(E) | Mouse Anti-S100A4 Recombinant Antibody; Fab Fragment (TAB-0136CL-F(E)) | WB, ELISA, BI, Block, Inhib | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-377CT | Anti-Human S100A4 Single Domain Antibody (TAB-377CT), Research Grade Biosimilar | WB, ELISA | Single domain antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-378CT | Anti-Human S100A4 Recombinant Antibody (TAB-378CT) | FuncS, IHC, Neut, Inhib | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-379CT | Mouse Anti-S100A4 Recombinant Antibody (TAB-379CT) | WB, IHC, Block | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-378CT-S(P) | Anti-Human S100A4 Recombinant Antibody scFv Fragment (5C3) | WB, IHC, Block |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-378CT-F(E) | Anti-Human S100A4 Recombinant Antibody Fab Fragment (5C3) | WB, IHC, Block |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1451MZ | Recombinant Mouse Anti-Human S100A4 Antibody (clone 1F12-1G7) | ELISA, ICC, IF, WB | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3155 | Hi-Affi™ Recombinant Rabbit Anti-S100A4 Monoclonal Antibody (DS3155AB) | WB, IHC, ICC, IP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC1028 | CytoStream™ Rabbit Anti-S100A4 Recombinant Antibody (VS13-YC1028) | WB, ICC, IF, IHC-P, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1992 | Anti-S100A4 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6304 | Anti-Mouse S100A4 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6303 | Anti-Human S100A4 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0625-YC283 | Recombinant Anti-S100A4 Eliminating Antibody, pH-Sensitive (VS-0625-YC283) | Antigen-Sweeping In Vivo. | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0825-YC299 | SmartAb™ Recombinant Anti-S100A4 pH-dependent Antibody (VS-0825-YC299) | ELISA | Human IgG |
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