Anti-Human S100A4 Recombinant Antibody (TAB-378CT)
CAT#: TAB-378CT
Recombinant monoclonal antibody specifically binds to human S100A4 (ELPSFLGKRT). This antibody has anti- angiogenic activity.
Published Data
Tested Data
Gene Expression
FuncS
Figure 1 Antitumor activity of 5C3.
PBS (negative control) or 5C3 (25 mg/kg) was given i.p. three times a week (1010100). At the end of the experiment, mice were sacrificed and tumors were weighted. Graphs of RTV shows the activity of 5C3 compared with the control group. Bars of tumor weight show the mean ± SEM. *p<0.05.
Hernandez, J. L., Padilla, L., Dakhel, S., Coll, T., Hervas, R., Adan, J., ... & Rodríguez, L. (2013). Therapeutic targeting of tumor growth and angiogenesis with a novel anti-S100A4 monoclonal antibody. PloS one, 8(9), e72480.
IHC
Figure 2 Immunohistologic analysis of tumor microvasculature from MiaPACA-2 tumors in animals treated with 5C3.
Quantifications were made from more than 10 pictures per slice at a magnification of X120 (2.4 mm²). Graphs show the mean ± SEM. *p<0.05.
Hernandez, J. L., Padilla, L., Dakhel, S., Coll, T., Hervas, R., Adan, J., ... & Rodríguez, L. (2013). Therapeutic targeting of tumor growth and angiogenesis with a novel anti-S100A4 monoclonal antibody. PloS one, 8(9), e72480.
Neut
Figure 3 5C3 (1-2 mM) neutralized the production of active forms of MMP-9 induced by S100A4 (arrowhead).
Each data point was normalized to the positive control of cells incubated with complete medium (left bar) that represents 100% migration. The control is the maximum of the possible migration (migration control). This migration is obtained by incubating HUVEC with basal medium containing 10% FCS plus hydrocortisone, brain bovine extract and hEGF. All the experimental points are referred to this control.
Hernandez, J. L., Padilla, L., Dakhel, S., Coll, T., Hervas, R., Adan, J., ... & Rodríguez, L. (2013). Therapeutic targeting of tumor growth and angiogenesis with a novel anti-S100A4 monoclonal antibody. PloS one, 8(9), e72480.
Inhib
Figure 4 Dose-response inhibition of S100A4-RAGE interaction by 5C3 mAb.
Antibody was used at 60–500 nM for 2 mM S100A4.
Hernandez, J. L., Padilla, L., Dakhel, S., Coll, T., Hervas, R., Adan, J., ... & Rodríguez, L. (2013). Therapeutic targeting of tumor growth and angiogenesis with a novel anti-S100A4 monoclonal antibody. PloS one, 8(9), e72480.
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WB
Figure 1 Mouse Anti-Human S100A4 Antibody (TAB-378CT) in WB
Western blot analysis of TAB-378CT was performed by loading Recombinant Human Protein S100A4 (expressed in E. coli).
TAB-378CT-L incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Anti-Mouse IgG (H+L)
Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.3 μg)
Lane 2: Reducing Antigen (0.6 μg)
ELISA
Figure 2 Mouse Anti-Human S100A4 Antibody (TAB-378CT) in ELISA
ELISA analysis of TAB-378CT was performed by coating with Recombinant Human Protein S100A4 (expressed in E. coli).
The secondary antibody: HRP-Anti-Mouse IgG (H+L)
DB
Figure 3 Mouse Anti-Human S100A4 Antibody (TAB-378CT) in Dot Blot
Dot Blot analysis of TAB-378CT was performed by coating with Recombinant Human Protein S100A4 (expressed in E. coli).
TAB-378CT-L incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Anti-Mouse IgG (H+L)
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Subcellular Location
Figure 1 IF staining of human cell line A549
Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7486/2064_B12_5_selected.jpg
Normal Tissue
Figure 2 Lung.
High expression in lung
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68227/152283_A_1_4_val_selected.jpg
Normal Tissue
Figure 3 Spleen
High expression in spleen
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7973/106662_B_7_4_val_selected.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7973/106662_A_7_3.jpg
Normal Tissue
Figure 5 Testis
Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7973/106662_A_6_6.jpg
Normal Tissue
Figure 6 Placenta
Decidual cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Trophoblastic cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7973/106662_A_2_7.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27387/106652_A_9_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (A549, ASC diff, BJ hTERT+, fHDF/TERT166, RPMI-8226)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000196154-S100A4
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Specifications
- Immunogen
- Full-length human S100A4
- Host Species
- Mouse
- Type
- Mouse IgG1, κ
- Specificity
- Human S100A4
- Species Reactivity
- Human, Mouse
- Applications
- FuncS, IHC, Neut, Inhib
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A/G purified
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Store at 4°C for short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Functional Assay, Immunohistochemistry, Neutralization, Inhibition.
Target
- Alternative Names
- S100A4; S100 calcium binding protein A4; 42A; 18A2; CAPL; FSP1; MTS1; P9KA; PEL98; protein S100-A4; protein Mts1; fibroblast-specific protein-1; placental calcium-binding protein; malignant transformation suppression 1; leukemia multidrug resistance associated protein; S100 calcium-binding protein A4 (calcium protein; calvasculin; metastasin; murine placental homolog); LK-1
- Gene ID
- 6275
- UniProt ID
- P26447
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
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Secondary Antibody
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Reconstitution Buffer
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Recommended Dilution Buffer
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-546CL | Human Anti-S100A4 Recombinant Antibody (TAB-546CL) | ELISA | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0135CL-S(P) | Mouse Anti-S100A4 Recombinant Antibody; scFv Fragment (TAB-0135CL-S(P)) | WB, ELISA, BI, Block, Inhib | Mouse scFv |
| TAB-379CT | Mouse Anti-S100A4 Recombinant Antibody (TAB-379CT) | WB, IHC, Block | Mouse IgG2a |
| TAB-380CT | Mouse Anti-S100A4 Recombinant Antibody (TAB-380CT) | WB, IHC, Block | Mouse IgG1 |
| TAB-381CT | Mouse Anti-S100A4 Recombinant Antibody (TAB-381CT) | WB, IHC, Block | Mouse IgG1 |
| TAB-382CT | Mouse Anti-S100A4 Recombinant Antibody (TAB-382CT) | WB, IHC, Block | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-377CT | Anti-Human S100A4 Single Domain Antibody (TAB-377CT), Research Grade Biosimilar | WB, ELISA | Single domain antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1451MZ | Recombinant Mouse Anti-Human S100A4 Antibody (clone 1F12-1G7) | ELISA, ICC, IF, WB | Mouse antibody |
| MRO-1358-CN | Recombinant Rabbit Anti-S100A4 Monoclonal Antibody (CBACN-494) | WB, IF, IHC, IP, FC | Rabbit IgG |
| VS3-QX994 | Mouse Anti-S100A4 Recombinant Antibody (clone 3D10-B4-D10) | WB, IP | Mouse IgG1 |
| VS3-FY1293 | Recombinant Mouse Anti-S100A4 Antibody (clone 3A1B2) | ELISA, IHC, FC | Mouse IgG1 |
| VS3-FY1294 | Recombinant Mouse Anti-S100A4 Antibody (clone 4A5A7) | ELISA, IHC, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3155 | Hi-Affi™ Recombinant Rabbit Anti-S100A4 Monoclonal Antibody (DS3155AB) | WB, IHC, ICC, IP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC1028 | CytoStream™ Rabbit Anti-S100A4 Recombinant Antibody (VS13-YC1028) | WB, ICC, IF, IHC-P, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1992 | Anti-S100A4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6304 | Anti-Mouse S100A4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6303 | Anti-Human S100A4 Immunohistochemistry Kit | IHC |
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