CytoStream™ Rabbit Anti-S100A4 Recombinant Antibody (VS13-YC1028) (CAT#: VS13-YC1028)
The CytoStream™ Rabbit Anti-S100A4 Recombinant Antibody is specific for human and mouse S100A4. S100A4 encodes a protein involved in cellular motility and tumor metastasis, existing in multiple splicing forms. The anti-S100A4 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

(Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7486/2064_B12_5_selected.jpg

(High expression in lung)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/68227/152283_A_1_4_val_selected.jpg

(High expression in spleen)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7973/106662_B_7_4_val_selected.jpg

(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7973/106662_A_7_3.jpg

(Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7973/106662_A_6_6.jpg

(Decidual cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Trophoblastic cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7973/106662_A_2_7.jpg

(Germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27387/106652_A_9_8.jpg

(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000196154-S100A4
Specifications
- Immunogen
- Recombinant human S100A4, aa 1-101.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse S100A4
- Species Reactivity
- Human, Mouse
- Applications
- WB, ICC, IF, IHC-P, IP, FC
- Conjugate
- NONE
- Related Disease
- Cardiovascular Disease
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/1000
Immunocytochemistry (ICC): 1/50-1/200
Immunofluorescence (IF): 1/50-1/200
Immunohistochemistry-paraffin (IHC-P): 1/50-1/200
Flow cytometry (FC): 1/50-1/100
Target
- Alternative Names
- S100 Calcium Binding Protein A4; S100 Calcium-Binding Protein A4 (Calcium Protein, Calvasculin, Metastasin, Murine Placental Homolog); Placental Calcium-Binding Protein; Fibroblast-Specific Protein-1; Protein Mts1; MTS1; CAPL; S100 Calcium Binding Protein A4 (Calcium Protein, Calvasculin, Metastasin, Murine Placental Homolog); Leukemia Multidrug Resistance Associated Protein; Malignant Transformation Suppression 1;
- Gene ID
- 6275
- UniProt ID
- P26447
- Long Name
- S100 Calcium Binding Protein A4
- Sequence Similarities
- Belongs to the S-100 family.
- Cellular Localization
- Extracellular space; Nucleus
- Protein Refseq
- NP_002952.1; NP_062427.1
- Function
- Calcium-binding protein that plays a role in various cellular processes including motility, angiogenesis, cell differentiation, apoptosis, and autophagy (PubMed:16707441, PubMed:23752197, PubMed:30713770).
Increases cell motility and invasiveness by interacting with non-muscle myosin heavy chain (NMMHC) IIA/MYH9 (PubMed:16707441).
Mechanistically, promotes filament depolymerization and increases the amount of soluble myosin-IIA, resulting in the formation of stable protrusions facilitating chemotaxis (By similarity).
Modulates also the pro-apoptotic function of TP53 by binding to its C-terminal transactivation domain within the nucleus and reducing its protein levels (PubMed:23752197).
Within the extracellular space, stimulates cytokine production including granulocyte colony-stimulating factor and CCL24 from T-lymphocytes (By similarity).
In addition, stimulates T-lymphocyte chemotaxis by acting as a chemoattractant complex with PGLYRP1 that promotes lymphocyte migration via CCR5 and CXCR3 receptors (PubMed:30713770, PubMed:26654597).
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "S100A4"
Select a product category from the dropdown menu below to view related products.
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-3568z | Mouse Anti-S100A4 Recombinant Antibody (clone 24D3) | WB, IP, IF, IHC, ELISA | Mouse IgG2a, κ |
TAB-378CT-S(P) | Anti-Human S100A4 Recombinant Antibody scFv Fragment (5C3) | WB, IHC, Block | |
MOB-1451MZ | Recombinant Mouse Anti-Human S100A4 Antibody (clone 1F12-1G7) | ELISA, ICC/IF, WB | Mouse antibody |
VS3-QX994 | Mouse Anti-S100A4 Recombinant Antibody (clone 3D10-B4-D10) | WB, IP | Mouse IgG1 |
VS-0224-XY90 | Rat Anti-Mouse S100a4 Recombinant Antibody (VS-0224-XY90) | ELISA, WB | Rat IgG2b |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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