Anti-Human TIM-3 Recombinant Antibody (mAb15) (CAT#: TAB-666CT)
Recombinant monoclonal antibody specifically binds to human TIM-3 and inhibits the interaction of TIM-3 and LILRB2. The antibody binds strongly to the CC' loop and mildly to the DE loop.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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Figure 1 show graphs demonstrating that TIM-3 inhibition stimulates expression of DC co-stimulatory molecules and cytokine release by DCs.
Following LPS activation, DCs were treated with no antibody, a mIgG1 isotype control, a commercially available anti-TIM-3 antibody (F38-2E2) or antibodies. Co-stimulatory molecules or cytokines were measured on Day 4 post-LPS activation.
Figure 2 show graphs demonstrating release of IL-1β, TNFα and IL-6 from macrophages stimulated by HMGB-1 following treatment with antibodies.
Antibodies were the commercially available anti-TIM-3 antibody, F38-2E2, and anti-TIM-3 antibody mAb15. mIgG1 served as an isotype negative control antibody.
Figure 3 shows a graph demonstrating a dose curve of release of TNFα from macrophages stimulated by HMGB-1 following treatment with different doses of antibodies.
Antibodies were the commercially available anti-TIM-3 antibody F38-2E2 (circles) and anti-TIM-3 antibody mAb15 (squares).
Figure 4 show graphs demonstrating dose curve of release of IL-1β from macrophages stimulated by LPS following treatment with different doses of antibodies.
Antibodies were the commercially available anti-TIM-3 antibody F38-2E2 (circles), anti-TIM-3 antibody mAb15 (squares) and anti-LILRB2 (clone 287219) (inverted triangles). mIgG1 isotype (diamonds) and no antibody (triangles) served as a negative control. Data were collected at 24 hr, 48 hr, and 3 days post-treatment as indicated.
Figure 5 shows mixed lymphocyte reaction on day 1 or day 7 following treatment with F38-2E2, mAb15 or an isotype control.
Supernatants were assessed for their expression of IL-1β, TNFα and IFN-γ at the time points indicated.
Specifications
- Immunogen
- Human TIM-3-Fc
- Host Species
- Mouse
- Derivation
- Mouse hybridoma cell line
- Specificity
- Human
- Species Reactivity
- Human
- Clone
- mAb15
- Applications
- ELISA, FC, Inhibit, FuncS
- Related Disease
- Acute Myelocytic Leukemia
Applications
- Application Notes
- The antibody was validated for Function assay. For details, refer to Published Data.
Target
- Alternative Names
- T-cell immunoglobulin and mucin domain containing molecule-3; TIM3; anti-TIM3
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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IgG Antibody Products
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Human Antibody Products
Mouse Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-976 | Recombinant Anti-Tim-3 Antibody | WB, Neut, FuncS | IgG |
| MHH-976 | Recombinant Human Anti-Tim-3 Antibody | ELISA, WB, FuncS | IgG |
| VS-0723-WK188 | Rabbit Anti-TIM3 Recombinant Antibody (VS-0723-WK188) | ELISA, FC | Rabbit IgG |
| VS-0723-WK280 | Rabbit Anti-TIM3 Recombinant Antibody (VS-0723-WK280) | ELISA, FC | Rabbit IgG |
| VS-0723-WK289 | Rabbit Anti-TIM3 Recombinant Antibody (VS-0723-WK289) | ELISA, FC | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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