Mouse Anti-TM4SF5 Recombinant Antibody (TAB-693CT) (CAT#: TAB-693CT)

Figure 1 Mouse Anti-TM4SF5 Recombinant Antibody (TAB-693CT) in SDS-PAGE

SDS-PAGE analysis of TAB-693CT in β-mercaptoethanol-reduced (Lane 1) and non-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.

Figure 2 Mouse Anti-TM4SF5 Recombinant Antibody (TAB-693CT) in SEC-HPLC

The purity of TAB-693CT was 99.79% as determined by SEC-HPLC.

Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl

Figure 3 Mouse Anti-TM4SF5 Recombinant Antibody (TAB-693CT) in FACS

Flow cytometry analysis of HepG2 cell with purified antibody TAB-693CT (Red). Cells (1x10^6) were incubated with TAB-693CT (Unconjugated, 50 μg/mL) for 30 min at 4°C. The FITC-goat anti-mouse IgG antibody was used as secondary antibody and incubated at a concentration of 20 μg/mL for 30 min at 4°C. The negative control group (Green) was only incubated with the secondary antibody.

Figure 4 Mouse Anti-TM4SF5 Recombinant Antibody (TAB-693CT-APC) detection with Nandrop300

Allophycocyanin (APC) has the absorption peaks among 610-665 nm wavelength. Take 2 ul of TAB-693CT-APC onto the lower measurement pedestal of Nandrop300 and the absorbance was read at 250 nm-750 nm. The result is shown on the left.

Figure 5 Mouse Anti-TM4SF5 Recombinant Antibody (TAB-693CT-APC) in SDS-PAGE

The TAB-693CT-unconjugated, TAB-693CT-APC and APC were loaded into the wells of the SDS-PAGE gel in non-reduced condition. And the fluorescence band was photographed at wavelength of 200 nm-650 nm.

Lane 1: TAB-693CT-unconjugated (2 ug)
Lane 2: TAB-693CT-APC (2 ug)
Lane 3: APC (2 ug)