Mouse Anti-TM4SF5 Recombinant Antibody (TAB-693CT)
CAT#: TAB-693CT
This product is a recombinant mouse antibody that recognizes TM4SF5. The antibody was expressed in mammalian cells with chemically defined culture media and was purified by affinity chromatography.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line Hep G2
Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm & cell junctions.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55674/1144_G10_1_selected.jpg
RNA Expression
Figure 2 RNA cell line category: Group enriched (CACO-2, Hep G2, OE19)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000142484-TM4SF5
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SDS-PAGE
Figure 1 Mouse Anti-TM4SF5 Recombinant Antibody (TAB-693CT) in SDS-PAGE
SDS-PAGE analysis of TAB-693CT in β-mercaptoethanol-reduced (Lane 1) and non-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
SEC-HPLC
Figure 2 Mouse Anti-TM4SF5 Recombinant Antibody (TAB-693CT) in SEC-HPLC
The purity of TAB-693CT was 99.79% as determined by SEC-HPLC.
Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl
FC
Figure 3 Mouse Anti-TM4SF5 Recombinant Antibody (TAB-693CT) in FACS
Flow cytometry analysis of HepG2 cell with purified antibody TAB-693CT (Red). Cells (1x10^6) were incubated with TAB-693CT (Unconjugated, 50 μg/mL) for 30 min at 4°C. The FITC-goat anti-mouse IgG antibody was used as secondary antibody and incubated at a concentration of 20 μg/mL for 30 min at 4°C. The negative control group (Green) was only incubated with the secondary antibody.
APC Absorb
Figure 4 Mouse Anti-TM4SF5 Recombinant Antibody (TAB-693CT-APC) detection with Nandrop300
Allophycocyanin (APC) has the absorption peaks among 610-665 nm wavelength. Take 2 ul of TAB-693CT-APC onto the lower measurement pedestal of Nandrop300 and the absorbance was read at 250 nm-750 nm. The result is shown on the left.
SDS-PAGE
Figure 5 Mouse Anti-TM4SF5 Recombinant Antibody (TAB-693CT-APC) in SDS-PAGE
The TAB-693CT-unconjugated, TAB-693CT-APC and APC were loaded into the wells of the SDS-PAGE gel in non-reduced condition. And the fluorescence band was photographed at wavelength of 200 nm-650 nm.
Lane 1: TAB-693CT-unconjugated (2 ug)
Lane 2: TAB-693CT-APC (2 ug)
Lane 3: APC (2 ug)
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Specifications
- Immunogen
- TM4SF5 extracellular domain 2 (AA113 to 157)
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human TM4SF5
- Species Reactivity
- Human
- Applications
- ELISA, FC, Inhib
- Related Disease
- Liver cancer
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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Reliable and Effective
The TM4SF5 antibody offers precise binding consistent across experiments, making it a staple in our lab's cancer research investigations.
Smooth Integration into Studies
The straightforward application process and high purity ensured smooth integration into our flow cytometry studies, generating accurate, reproducible data.
Consistent Results Every Time
Whether using it for ELISA or inhibition assays, the results are consistently reliable, advancing our projects with confidence.
Q&As
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What are the main applications of the TM4SF5 antibody?
A: This antibody is primarily used in ELISA, flow cytometry, and inhibition studies. Its ability to bind TM4SF5 helps explore its expression in various cellular contexts.
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How is it conjugated for different experiments?
A: You can choose from conjugation options like PE, APC, and Biotin, allowing the antibody to be flexible for different assay requirements.
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Can I customize the quantities I order?
A: Yes, Creative Biolabs offers flexibility in sizes from micrograms to grams, ensuring you receive only what you need for your specific research needs.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "TM4SF5"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-693CT-F(E) | Human Anti-TM4SF5 Recombinant Antibody; Fab Fragment (TAB-693CT-F(E)) | ELISA, FC, Inhib | Human Fab |
| TAB-694CT-F(E) | Human Anti-TM4SF5 Recombinant Antibody; Fab Fragment (TAB-694CT-F(E)) | ELISA, FC, Inhib | Human Fab |
| TAB-695CT-F(E) | Human Anti-TM4SF5 Recombinant Antibody; Fab Fragment (TAB-695CT-F(E)) | ELISA, FC, Inhib | Human Fab |
| TAB-696CT-F(E) | Human Anti-TM4SF5 Recombinant Antibody; Fab Fragment (TAB-696CT-F(E)) | ELISA, FC, Inhib | Human Fab |
| TAB-697CT-F(E) | Human Anti-TM4SF5 Recombinant Antibody; Fab Fragment (TAB-697CT-F(E)) | ELISA, FC, Inhib | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0350MC | Rabbit Anti-TM4SF5 Polyclonal Antibody | ELISA, IHC | Rabbit IgG |
| HPAB-N0263-YC | Human Anti-TM4SF5 Recombinant Antibody (HPAB-N0263-YC) | ELISA, FC, Inhib, FuncS | Humanized IgG |
| HPAB-0103-WJ | Human Anti-TM4SF5 Recombinant Antibody (HPAB-0103-WJ) | ELISA, SPR, IHC, Inhib, IF, WB | Human IgG2a, κ |
| FAMAB-0706WJ | Mouse Anti-TM4SF5 Recombinant Antibody (clone mEC2-C) | ELISA, WB | Mouse IgG |
| FAMAB-0707WJ | Human Anti-TM4SF5 Recombinant Antibody (clone hEC2-C-1) | ELISA, WB | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-N0263-YC-S(P) | Human Anti-TM4SF5 Recombinant Antibody; scFv Fragment (HPAB-N0263-YC-S(P)) | ELISA, FC, Inhib, FuncS | Humanized scFv |
| HPAB-0103-WJ-S(P) | Mouse Anti-TM4SF5 Recombinant Antibody; scFv Fragment (HPAB-0103-WJ-S(P)) | ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-N0263-YC-F(E) | Human Anti-TM4SF5 Recombinant Antibody; Fab Fragment (HPAB-N0263-YC-F(E)) | ELISA, FC, Inhib, FuncS | Humanized Fab |
| HPAB-0103-WJ-F(E) | Human Anti-TM4SF5 Recombinant Antibody; Fab Fragment (HPAB-0103-WJ-F(E)) | ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY172 | Human Anti-TM4SF5 (clone hEC2-C-2) scFv-Fc Chimera | ELISA, WB, IP, IF, IHC | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC101 | Recombinant Anti-TM4SF5 Vesicular Antibody, EV Displayed (VS-0425-YC101) | ELISA, FC, Inhib, Cell-uptake |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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