Mouse Anti-VWF Recombinant Antibody (clone 82D6A3)
CAT#: PABZ-132
Recombinant Mouse Antibody (82D6A3) is capable of binding to VWF, expressed in Chinese Hamster Ovary cells (CHO). This antibody inhibits the VWF-collagen interaction through binding to the VWF A3-domain, is a powerful antithrombotic agent.
Published Data
Gene Expression
Tested Data
Figure 2 Effect of 82D6A3 on platelet adhesion under flow conditions.
Platelet deposition onto a human collagen type III-coated surface was studied as a function of the concentration of mAb 82D6A3 at a shear rate 2600 s⁻¹ (A) and as a function of the shear rate (B). Closed bars, no antibody; open bars, 2.3 μg/ml 82D6A3. Data are mean ± S.D. (n= 4, with blood from two different donors).
Vanhoorelbeke, K., Depraetere, H., Romijn, R. A., Huizinga, E. G., De Maeyer, M., & Deckmyn, H. (2003). A consensus tetrapeptide selected by phage display adopts the conformation of a dominant discontinuous epitope of a monoclonal anti-VWF antibody that inhibits the von Willebrand factor-collagen interaction. Journal of Biological Chemistry, 278(39), 37815-37821.
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Normal Tissue
Figure 1 Cerebral cortex
Endothelial cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1694/4967_B_8_5.jpg
Normal Tissue
Figure 2 Colon
Endothelial cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1694/4967_A_9_3.jpg
RNA Expression
Figure 3 RNA cell line category: Cell line enriched (HUVEC TERT2)
Cell lines ordered by descending RNA expression order.
RNA Expression
Figure 3 RNA cell line category: Cell line enriched (HUVEC TERT2)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000110799-VWF
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SDS-PAGE
Figure 1 Mouse Anti-VWF Recombinant Antibody (PABZ-132) in SDS-PAGE
SDS-PAGE analysis of PABZ-132 in non-reduced (Lane 2) and reduced (Lane 1) conditions.
SEC-HPLC
Figure 2 Mouse Anti-VWF Recombinant Antibody (PABZ-132) in SEC-HPLC
SEC-HPLC analysis of PABZ-132 shows that the purity is more than 95%.
ELISA
Figure 3 Mouse Anti-VWF Recombinant Antibody (PABZ-132) in ELISA
ELISA analysis of PABZ-132 was performed by coating with Von Willebrand Factor/vWF Protein, Human, Recombinant (His Tag).
The secondary antibody: Goat Anti-Mouse IgG(H+L)-HRP
WB
Figure 4 Mouse Anti-VWF Recombinant Antibody (PABZ-132) in WB
Western blot analysis of PABZ-132 was performed with Von Willebrand Factor/vWF Protein, Human, Recombinant (His Tag).
PABZ-132 incubation concentration: 2ng/μL.
The secondary antibody: Goat Anti-Mouse IgG(H+L)-HRP
Lane 1: Reducing Antigen (0.1μg)
Lane 2: Reducing Antigen (0.3μg)
Lane 3: Reducing Antigen (0.6μg)
DB
Figure 5 Mouse Anti-VWF Recombinant Antibody (PABZ-132) in DB
Dot Blot analysis of PABZ-132 was performed by coating with Von Willebrand Factor/vWF Protein, Human, Recombinant (His Tag).
PABZ-132 incubation concentration: 2ng/μL.
The secondary antibody: Goat Anti-Mouse IgG(H+L)-HRP
NC: Negative control PC: Positive control
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Specifications
- Immunogen
- Human Von Willebrand Factor
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human VWF
- Species Reactivity
- Human
- Clone
- 82D6A3
- Applications
- WB, IF, FuncS
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody 82D6A3 has been reported in applications of Inhib, IF, WB, FuncS. It's recommended that the optimal antibody concentration, dilution, incubition time etc. are best to be carefully titrated in specific assays.
Target
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Reliable and Consistent Results
I've consistently obtained reliable results using this antibody. The specificity and affinity for VWF lead to clean detection, which is critical for my ongoing studies into bleeding disorders. Highly recommend it for anyone working in vascular biology.
Excellent Specificity for VWF
The Mouse Anti-VWF Recombinant Antibody (clone 82D6A3) has shown exceptional specificity in my assays. It provides clear bands in Western Blots and reliable results in functional studies, making it an invaluable tool for studying von Willebrand factor in hemostatic research.
Q&As
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What are the recommended storage conditions for long-term use?
A: For long-term storage, the antibody should be stored at -20°C. Avoiding repeated freeze-thaw cycles is important to maintain its functional integrity. For short-term use (1-2 weeks), it can be stored at +4°C.
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What technical support does Creative Biolabs provide for this product?
A: Creative Biolabs offers extensive technical support for users of the Mouse Anti-VWF Recombinant Antibody, including experimental design assistance, troubleshooting, and protocol optimization to ensure researchers achieve reliable and high-quality results.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# PABZ-132, RRID: AB_3111708)
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "Clone 82D6A3"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1149z | Mouse Anti-VWF Recombinant Antibody (clone 41C2) | WB, FC, IF, IHC, IP | Mouse IgG1, κ |
| MOB-2032CT | Recombinant Mouse anti-Human VWF Monoclonal antibody (47C22) | IHC-Fr, IHC-P | |
| MOB-177CQ | Mouse Anti-VWF Recombinant Antibody (clone YC161) | WB, IP, IF, IHC-P | Mouse IgG1, κ |
| HPAB-M0250-YC | Mouse Anti-VWF Recombinant Antibody (clone SZ123) | Inhib, FuncS | Mouse IgG |
| HPAB-M0251-YC | Human Anti-VWF Recombinant Antibody (clone MHC-SZ123) | Inhib, FuncS | Chimeric (mouse/human) IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBZ-132 | Mouse Anti-VWF Recombinant Antibody (clone 82D6A3); Fab Fragment | WB, IF, FuncS | Mouse Fab |
| PFBW-132 | Mouse Anti-VWF Recombinant Antibody (clone NMC-4); Fab Fragment | Block | Mouse Fab |
| PFBC-133 | Mouse Anti-VWF Recombinant Antibody (clone RU5); Fab Fragment | WB, IHC, IF, FuncS | Mouse Fab |
| HPAB-M0250-YC-F(E) | Mouse Anti-VWF Recombinant Antibody (clone SZ123); Fab Fragment | Inhib, FuncS | Mouse Fab |
| HPAB-M0251-YC-F(E) | Human Anti-VWF Recombinant Antibody (clone MHC-SZ123); Fab Fragment | Inhib, FuncS | Chimeric (mouse/human) Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBZ-132 | Mouse Anti-VWF Recombinant Antibody (clone 82D6A3); scFv Fragment | WB, IF, FuncS | Mouse scFv |
| PSBW-132 | Mouse Anti-VWF Recombinant Antibody (clone NMC-4); scFv Fragment | Block | Mouse scFv |
| FAMAB-0297-YC-S(P) | Human Anti-VWF Recombinant Antibody (clone h82D6A3); scFv Fragment | ELISA, WB | Humanized scFv |
| FAMAB-0298-YC-S(P) | Mouse Anti-VWF Recombinant Antibody (clone m-82D6A3); scFv Fragment | ELISA, WB | Mouse scFv |
| HPAB-0343-WJ-S(P) | Mouse Anti-VWF Recombinant Antibody; scFv Fragment (HPAB-0343-WJ-S(P)) | ELISA, WB | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-362CQ | Anti-Human VWF Recombinant Antibody (AJvW-2) | ELISA, BL | |
| TAB-362CQ-S(P) | Anti-Human VWF Recombinant Antibody scFv Fragment (AJvW-2) | ELISA, BL | |
| TAB-363CQ-S(P) | Mouse Anti-VWF Recombinant Antibody; scFv Fragment (TAB-363CQ-S(P)) | ELISA, WB, Inhib | Mouse scFv |
| TAB-362CQ-F(E) | Anti-Human VWF Recombinant Antibody Fab Fragment (AJvW-2) | ELISA, BL | |
| TAB-363CQ-F(E) | Mouse Anti-VWF Recombinant Antibody; Fab Fragment (TAB-363CQ-F(E)) | ELISA, WB, Inhib | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-364CQ | Human Anti-VWF Recombinant Antibody (TAB-364CQ) | Inhib | Humanized antibody |
| TAB-364CQ-S(P) | Human Anti-VWF Recombinant Antibody; scFv Fragment (TAB-364CQ-S(P)) | Inhib | Humanized scFv |
| TAB-364CQ-F(E) | Human Anti-VWF Recombinant Antibody; Fab Fragment (TAB-364CQ-F(E)) | Inhib | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-365CQ | Human Anti-VWF Recombinant Antibody (TAB-365CQ) | Inhib | Chimeric antibody (mouse/human) |
| TAB-365CQ-S(P) | Mouse Anti-VWF Recombinant Antibody; scFv Fragment (TAB-365CQ-S(P)) | Inhib | Mouse scFv |
| TAB-365CQ-F(E) | Human Anti-VWF Recombinant Antibody; Fab Fragment (TAB-365CQ-F(E)) | Inhib | Chimeric antibody (mouse/human) |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3860 | Rabbit Anti-VWF Recombinant Antibody (clone DS3860AB) | WB, ICC, IF | Rabbit IgG |
| MOR-0109-FY | Rabbit Anti-VWF Recombinant Antibody (clone AFY0080) | ICC, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1628-FY | Llama Anti-VWF Recombinant Single Domain Antibody (HPAB-1628-FY) | ELISA | Llama VHH |
| HPAB-1629-FY | Llama Anti-VWF Recombinant Single Domain Antibody (HPAB-1629-FY) | ELISA | Llama VHH |
| HPAB-1630-FY | Llama Anti-VWF Recombinant Single Domain Antibody (HPAB-1630-FY) | Inhib, ELISA | Llama VHH |
| HPAB-1631-FY | Recombinant Llama Anti-VWF Single Domain Antibody (HPAB-1631-FY) | ELISA, SPR, FC | Llama VHH |
| HPAB-1632-FY | Recombinant Llama Anti-VWF Single Domain Antibody (HPAB-1632-FY) | Inhib | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0125-FY41 | Human Anti-vWF (clone AJvW-2) scFv-Fc Chimera | ELISA, Block | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2481 | Anti-VWF Immunohistochemistry Kit | IHC | |
| VS-0525-XY7888 | Anti-Mouse VWF Immunohistochemistry Kit | IHC | |
| VS-0525-XY7887 | Anti-Human VWF Immunohistochemistry Kit | IHC |
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