What are the recommended storage conditions for long-term use?

For long-term storage, the antibody should be stored at -20°C. Avoiding repeated freeze-thaw cycles is important to maintain its functional integrity. For short-term use (1-2 weeks), it can be stored at +4°C.

What technical support does Creative Biolabs provide for this product?

Creative Biolabs offers extensive technical support for users of the Mouse Anti-VWF Recombinant Antibody, including experimental design assistance, troubleshooting, and protocol optimization to ensure researchers achieve reliable and high-quality results.

Mouse Anti-VWF Recombinant Antibody (clone 82D6A3) (CAT#: PABZ-132)

Recombinant Mouse Antibody (82D6A3) is capable of binding to VWF, expressed in Chinese Hamster Ovary cells (CHO). This antibody inhibits the VWF-collagen interaction through binding to the VWF A3-domain, is a powerful antithrombotic agent.

Custom Production

We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

We require custom production

Published Data
Tested Data
Gene Expression
Datasheet
MSDS
COA

Figure 2 Effect of 82D6A3 on platelet adhesion under flow conditions.

Figure 2 Effect of 82D6A3 on platelet adhesion under flow conditions.

Platelet deposition onto a human collagen type III-coated surface was studied as a function of the concentration of mAb 82D6A3 at a shear rate 2600 s⁻¹ (A) and as a function of the shear rate (B). Closed bars, no antibody; open bars, 2.3 μg/ml 82D6A3. Data are mean ± S.D. (n= 4, with blood from two different donors).

Vanhoorelbeke, K., Depraetere, H., Romijn, R. A., Huizinga, E. G., De Maeyer, M., & Deckmyn, H. (2003). A consensus tetrapeptide selected by phage display adopts the conformation of a dominant discontinuous epitope of a monoclonal anti-VWF antibody that inhibits the von Willebrand factor-collagen interaction. Journal of Biological Chemistry, 278(39), 37815-37821.

SDS-PAGE

Figure 1 Mouse Anti-VWF Recombinant Antibody (PABZ-132) in SDS-PAGE

Figure 1 Mouse Anti-VWF Recombinant Antibody (PABZ-132) in SDS-PAGE

SDS-PAGE analysis of PABZ-132 in non-reduced (Lane 2) and reduced (Lane 1) conditions.

SEC-HPLC

Figure 2 Mouse Anti-VWF Recombinant Antibody (PABZ-132) in SEC-HPLC

Figure 2 Mouse Anti-VWF Recombinant Antibody (PABZ-132) in SEC-HPLC

SEC-HPLC analysis of PABZ-132 shows that the purity is more than 95%.

ELISA

Figure 3 Mouse Anti-VWF Recombinant Antibody (PABZ-132) in ELISA

Figure 3 Mouse Anti-VWF Recombinant Antibody (PABZ-132) in ELISA

ELISA analysis of PABZ-132 was performed by coating with Von Willebrand Factor/vWF Protein, Human, Recombinant (His Tag).
The secondary antibody: Goat Anti-Mouse IgG(H+L)-HRP

Figure 4 Mouse Anti-VWF Recombinant Antibody (PABZ-132) in WB

Figure 4 Mouse Anti-VWF Recombinant Antibody (PABZ-132) in WB

Western blot analysis of PABZ-132 was performed with Von Willebrand Factor/vWF Protein, Human, Recombinant (His Tag).
PABZ-132 incubation concentration: 2ng/μL.
The secondary antibody: Goat Anti-Mouse IgG(H+L)-HRP
Lane 1: Reducing Antigen (0.1μg)
Lane 2: Reducing Antigen (0.3μg)
Lane 3: Reducing Antigen (0.6μg)

Figure 5 Mouse Anti-VWF Recombinant Antibody (PABZ-132) in DB

Figure 5 Mouse Anti-VWF Recombinant Antibody (PABZ-132) in DB

Dot Blot analysis of PABZ-132 was performed by coating with Von Willebrand Factor/vWF Protein, Human, Recombinant (His Tag).
PABZ-132 incubation concentration: 2ng/μL.
The secondary antibody: Goat Anti-Mouse IgG(H+L)-HRP
NC: Negative control PC: Positive control

Normal Tissue

Figure 1 Cerebral cortex
(Endothelial cells
Staining:

High

Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1694/4967_B_8_5.jpg

Figure 2 Colon
(Endothelial cells
Staining:

High

Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1694/4967_A_9_3.jpg

RNA Expression

Figure 3 RNA cell line category: Cell line enriched (HUVEC TERT2)
(Cell lines ordered by descending RNA expression order.)
* https://v21.proteinatlas.org/ENSG00000110799-VWF

Figure 3 RNA cell line category: Cell line enriched (HUVEC TERT2)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000110799-VWF

Datasheet

MSDS

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Immunogen
Human Von Willebrand Factor

Host Species
Mouse

Type
Mouse IgG

Specificity
Human VWF

Species Reactivity
Human

Clone
82D6A3

Applications
WB, IF, FuncS

Product Property

Purity
>95% as determined by SDS-PAGE and HPLC analysis

Concentration
Please refer to the vial label for the specific concentration.

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
The antibody 82D6A3 has been reported in applications of Inhib, IF, WB, FuncS. It's recommended that the optimal antibody concentration, dilution, incubition time etc. are best to be carefully titrated in specific assays.

Target

Alternative Names
VWF; VWD; Von Willebrand Factor; Coagulation Factor VIII VWF; VWF

Gene ID
7450

UniProt ID
P04275

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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CAT	Product Name	Application	Type
PABW-132	Mouse Anti-VWF Recombinant Antibody (clone NMC-4)	Block, Inhib, WB, DB	Mouse IgG
PABC-133	Mouse Anti-vWF Recombinant Antibody (clone RU5)	ELISA, WB, IHC, IF, Inhib, FuncS	Mouse IgG
MOB-2032CT	Recombinant Mouse anti-Human VWF Monoclonal antibody (47C22)	IHC-Fr, IHC-P
HPAB-0343-WJ	Mouse Anti-VWF Recombinant Antibody (HPAB-0343-WJ)	ELISA, WB	Mouse IgG
VS3-FY1936	Rabbit Anti-VWF Recombinant Antibody (clone R01-5D2)	WB, IHC-P	Rabbit IgG

CAT	Product Name	Application	Type
PFBZ-132	Mouse Anti-VWF Recombinant Antibody (clone 82D6A3); Fab Fragment	WB, IF, FuncS	Mouse Fab
PFBW-132	Mouse Anti-VWF Recombinant Antibody (clone NMC-4); Fab Fragment	Block	Mouse Fab
PFBC-133	Mouse Anti-VWF Recombinant Antibody (clone RU5); Fab Fragment	WB, IHC, IF, FuncS	Mouse Fab
FAMAB-0297-YC-F(E)	Human Anti-VWF Recombinant Antibody (clone h82D6A3); Fab Fragment	ELISA, WB	Humanized Fab
HPAB-0343-WJ-F(E)	Mouse Anti-VWF Recombinant Antibody; Fab Fragment (HPAB-0343-WJ-F(E))	ELISA, WB	Mouse Fab

CAT	Product Name	Application	Type
PSBZ-132	Mouse Anti-VWF Recombinant Antibody (clone 82D6A3); scFv Fragment	WB, IF, FuncS	Mouse scFv
PSBW-132	Mouse Anti-VWF Recombinant Antibody (clone NMC-4); scFv Fragment	Block	Mouse scFv
PSBC-133	Mouse Anti-VWF Recombinant Antibody (clone RU5); scFv Fragment	WB, IHC, IF, FuncS	Mouse scFv
HPAB-M0250-YC-S(P)	Mouse Anti-VWF Recombinant Antibody (clone SZ123); scFv Fragment	Inhib, FuncS	Mouse scFv
HPAB-M0251-YC-S(P)	Human Anti-VWF Recombinant Antibody (clone MHC-SZ123); scFv Fragment	Inhib, FuncS	Mouse scFv

CAT	Product Name	Application	Type
TAB-362CQ	Anti-Human VWF Recombinant Antibody (AJvW-2)	ELISA, BL
TAB-362CQ-S(P)	Anti-Human VWF Recombinant Antibody scFv Fragment (AJvW-2)	ELISA, BL
TAB-363CQ-S(P)	Mouse Anti-VWF Recombinant Antibody; scFv Fragment (TAB-363CQ-S(P))	ELISA, WB, Inhib	Mouse scFv
TAB-362CQ-F(E)	Anti-Human VWF Recombinant Antibody Fab Fragment (AJvW-2)	ELISA, BL
TAB-363CQ-F(E)	Mouse Anti-VWF Recombinant Antibody; Fab Fragment (TAB-363CQ-F(E))	ELISA, WB, Inhib	Mouse Fab

CAT	Product Name	Application	Type
TAB-364CQ	Human Anti-VWF Recombinant Antibody (TAB-364CQ)	Inhib	Humanized antibody
TAB-364CQ-S(P)	Human Anti-VWF Recombinant Antibody; scFv Fragment (TAB-364CQ-S(P))	Inhib	Humanized scFv
TAB-364CQ-F(E)	Human Anti-VWF Recombinant Antibody; Fab Fragment (TAB-364CQ-F(E))	Inhib	Humanized Fab

CAT	Product Name	Application	Type
TAB-365CQ	Human Anti-VWF Recombinant Antibody (TAB-365CQ)	Inhib	Chimeric antibody (mouse/human)
TAB-365CQ-S(P)	Mouse Anti-VWF Recombinant Antibody; scFv Fragment (TAB-365CQ-S(P))	Inhib	Mouse scFv
TAB-365CQ-F(E)	Human Anti-VWF Recombinant Antibody; Fab Fragment (TAB-365CQ-F(E))	Inhib	Chimeric antibody (mouse/human)

CAT	Product Name	Application	Type
MOR-3860	Rabbit Anti-VWF Recombinant Antibody (clone DS3860AB)	WB, ICC, IF	Rabbit IgG
MOR-0109-FY	Rabbit Anti-VWF Recombinant Antibody (clone AFY0080)	ICC, IHC-P, WB	Rabbit IgG

CAT	Product Name	Application	Type
HPAB-1628-FY	Llama Anti-VWF Recombinant Single Domain Antibody (HPAB-1628-FY)	ELISA	Llama VHH
HPAB-1629-FY	Llama Anti-VWF Recombinant Single Domain Antibody (HPAB-1629-FY)	ELISA	Llama VHH
HPAB-1630-FY	Llama Anti-VWF Recombinant Single Domain Antibody (HPAB-1630-FY)	Inhib, ELISA	Llama VHH
HPAB-1631-FY	Recombinant Llama Anti-VWF Single Domain Antibody (HPAB-1631-FY)	ELISA, SPR, FC	Llama VHH
HPAB-1632-FY	Recombinant Llama Anti-VWF Single Domain Antibody (HPAB-1632-FY)	Inhib	Llama VHH

CAT	Product Name	Application	Type
VS-0125-FY41	Human Anti-vWF (clone AJvW-2) scFv-Fc Chimera	ELISA, Block	Human IgG1, scFv-Fc

CAT	Product Name	Application	Type
VS-0325-XY2481	Anti-VWF Immunohistochemistry Kit	IHC

Customer Reviews and Q&As

Customer Review Q&As

Reliable and Consistent Results

I've consistently obtained reliable results using this antibody. The specificity and affinity for VWF lead to clean detection, which is critical for my ongoing studies into bleeding disorders. Highly recommend it for anyone working in vascular biology.

Excellent Specificity for VWF

The Mouse Anti-VWF Recombinant Antibody (clone 82D6A3) has shown exceptional specificity in my assays. It provides clear bands in Western Blots and reliable results in functional studies, making it an invaluable tool for studying von Willebrand factor in hemostatic research.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# PABZ-132, RRID: AB_3111708)

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