What are the recommended storage conditions for long-term use?

For long-term storage, the antibody should be stored at -20°C. Avoiding repeated freeze-thaw cycles is important to maintain its functional integrity. For short-term use (1-2 weeks), it can be stored at +4°C.

What technical support does Creative Biolabs provide for this product?

Creative Biolabs offers extensive technical support for users of the Mouse Anti-VWF Recombinant Antibody, including experimental design assistance, troubleshooting, and protocol optimization to ensure researchers achieve reliable and high-quality results.

Mouse Anti-VWF Recombinant Antibody (clone 82D6A3)

CAT#: PABZ-132

Recombinant Mouse Antibody (82D6A3) is capable of binding to VWF, expressed in Chinese Hamster Ovary cells (CHO). This antibody inhibits the VWF-collagen interaction through binding to the VWF A3-domain, is a powerful antithrombotic agent.

Tested Data Published Data Gene Expression

SDS-PAGE

Figure 1 Mouse Anti-VWF Recombinant Antibody (PABZ-132) in SDS-PAGE

SDS-PAGE analysis of PABZ-132 in non-reduced (Lane 2) and reduced (Lane 1) conditions.

SEC-HPLC

Figure 2 Mouse Anti-VWF Recombinant Antibody (PABZ-132) in SEC-HPLC

SEC-HPLC analysis of PABZ-132 shows that the purity is more than 95%.

ELISA

Figure 3 Mouse Anti-VWF Recombinant Antibody (PABZ-132) in ELISA

ELISA analysis of PABZ-132 was performed by coating with Von Willebrand Factor/vWF Protein, Human, Recombinant (His Tag).
The secondary antibody: Goat Anti-Mouse IgG(H+L)-HRP

Figure 4 Mouse Anti-VWF Recombinant Antibody (PABZ-132) in WB

Western blot analysis of PABZ-132 was performed with Von Willebrand Factor/vWF Protein, Human, Recombinant (His Tag).
PABZ-132 incubation concentration: 2ng/μL.
The secondary antibody: Goat Anti-Mouse IgG(H+L)-HRP
Lane 1: Reducing Antigen (0.1μg)
Lane 2: Reducing Antigen (0.3μg)
Lane 3: Reducing Antigen (0.6μg)

Figure 5 Mouse Anti-VWF Recombinant Antibody (PABZ-132) in DB

Dot Blot analysis of PABZ-132 was performed by coating with Von Willebrand Factor/vWF Protein, Human, Recombinant (His Tag).
PABZ-132 incubation concentration: 2ng/μL.
The secondary antibody: Goat Anti-Mouse IgG(H+L)-HRP
NC: Negative control PC: Positive control

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Figure 2 Effect of 82D6A3 on platelet adhesion under flow conditions.

Platelet deposition onto a human collagen type III-coated surface was studied as a function of the concentration of mAb 82D6A3 at a shear rate 2600 s⁻¹ (A) and as a function of the shear rate (B). Closed bars, no antibody; open bars, 2.3 μg/ml 82D6A3. Data are mean ± S.D. (n= 4, with blood from two different donors).

Vanhoorelbeke, K., Depraetere, H., Romijn, R. A., Huizinga, E. G., De Maeyer, M., & Deckmyn, H. (2003). A consensus tetrapeptide selected by phage display adopts the conformation of a dominant discontinuous epitope of a monoclonal anti-VWF antibody that inhibits the von Willebrand factor-collagen interaction. Journal of Biological Chemistry, 278(39), 37815-37821.

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Normal Tissue Figure 1 Cerebral cortex

Endothelial cells
Staining:

High

Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1694/4967_B_8_5.jpg

Normal Tissue Figure 2 Colon

Endothelial cells
Staining:

High

Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1694/4967_A_9_3.jpg

RNA Expression Figure 3 RNA cell line category: Cell line enriched (HUVEC TERT2)

Cell lines ordered by descending RNA expression order.

RNA Expression Figure 3 RNA cell line category: Cell line enriched (HUVEC TERT2)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000110799-VWF

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Specifications

Immunogen
Human Von Willebrand Factor

Host Species
Mouse

Type
Mouse IgG

Specificity
Human VWF

Species Reactivity
Human

Clone
82D6A3

Applications
WB, IF, FuncS

Product Property

Purity
>95% as determined by SDS-PAGE and HPLC analysis

Concentration
Please refer to the vial label for the specific concentration.

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
The antibody 82D6A3 has been reported in applications of Inhib, IF, WB, FuncS. It's recommended that the optimal antibody concentration, dilution, incubition time etc. are best to be carefully titrated in specific assays.

Target

Alternative Names
VWF; VWD; Von Willebrand Factor; Coagulation Factor VIII VWF; VWF

Gene ID
7450

UniProt ID
P04275

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Breast cancer biomarkers at key points during disease progression

Reliable and Consistent Results

I've consistently obtained reliable results using this antibody. The specificity and affinity for VWF lead to clean detection, which is critical for my ongoing studies into bleeding disorders. Highly recommend it for anyone working in vascular biology.

Excellent Specificity for VWF

The Mouse Anti-VWF Recombinant Antibody (clone 82D6A3) has shown exceptional specificity in my assays. It provides clear bands in Western Blots and reliable results in functional studies, making it an invaluable tool for studying von Willebrand factor in hemostatic research.

Q&As

What are the recommended storage conditions for long-term use?

A: For long-term storage, the antibody should be stored at -20°C. Avoiding repeated freeze-thaw cycles is important to maintain its functional integrity. For short-term use (1-2 weeks), it can be stored at +4°C.
What technical support does Creative Biolabs provide for this product?

A: Creative Biolabs offers extensive technical support for users of the Mouse Anti-VWF Recombinant Antibody, including experimental design assistance, troubleshooting, and protocol optimization to ensure researchers achieve reliable and high-quality results.

View the frequently asked questions answered by Creative Biolabs Support.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# PABZ-132, RRID: AB_3111708)

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Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - PABZ-132.pdf

MSDS

MSDS - PABZ-132.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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