Mouse Anti-VWF Recombinant Antibody (clone 82D6A3) (CAT#: PABZ-132)

Figure 2 Effect of 82D6A3 on platelet adhesion under flow conditions.

Platelet deposition onto a human collagen type III-coated surface was studied as a function of the concentration of mAb 82D6A3 at a shear rate 2600 s⁻¹ (A) and as a function of the shear rate (B). Closed bars, no antibody; open bars, 2.3 μg/ml 82D6A3. Data are mean ± S.D. (n= 4, with blood from two different donors).

Vanhoorelbeke, K., Depraetere, H., Romijn, R. A., Huizinga, E. G., De Maeyer, M., & Deckmyn, H. (2003). A consensus tetrapeptide selected by phage display adopts the conformation of a dominant discontinuous epitope of a monoclonal anti-VWF antibody that inhibits the von Willebrand factor-collagen interaction. Journal of Biological Chemistry, 278(39), 37815-37821.

Figure 1 Mouse Anti-VWF Recombinant Antibody (PABZ-132) in SDS-PAGE

SDS-PAGE analysis of PABZ-132 in non-reduced (Lane 2) and reduced (Lane 1) conditions.

Figure 2 Mouse Anti-VWF Recombinant Antibody (PABZ-132) in SEC-HPLC

SEC-HPLC analysis of PABZ-132 shows that the purity is more than 95%.

Figure 3 Mouse Anti-VWF Recombinant Antibody (PABZ-132) in ELISA

ELISA analysis of PABZ-132 was performed by coating with Von Willebrand Factor/vWF Protein, Human, Recombinant (His Tag).
The secondary antibody: Goat Anti-Mouse IgG(H+L)-HRP

Figure 4 Mouse Anti-VWF Recombinant Antibody (PABZ-132) in WB

Western blot analysis of PABZ-132 was performed with Von Willebrand Factor/vWF Protein, Human, Recombinant (His Tag).
PABZ-132 incubation concentration: 2ng/μL.
The secondary antibody: Goat Anti-Mouse IgG(H+L)-HRP
Lane 1: Reducing Antigen (0.1μg)
Lane 2: Reducing Antigen (0.3μg)
Lane 3: Reducing Antigen (0.6μg)

Figure 5 Mouse Anti-VWF Recombinant Antibody (PABZ-132) in DB

Dot Blot analysis of PABZ-132 was performed by coating with Von Willebrand Factor/vWF Protein, Human, Recombinant (His Tag).
PABZ-132 incubation concentration: 2ng/μL.
The secondary antibody: Goat Anti-Mouse IgG(H+L)-HRP
NC: Negative control PC: Positive control