IL-18-DT immunotoxin
CAT#: AGTO-G042D
This immunotoxin was achieved by conjugating/fusing the IL-18 to Diphtheria toxin. It was expressed in CHO and purified with affinity chromatography. The immunotoxin selectively binds to IL-18R–bearing antigen-presenting cells (APCs) and the toxin enters cells by receptor-mediated endocytosis. Before the N-terminal 193 amino acids corresponding to the catalytic domain of DT enzyme, -II binding and elongation factor into the eukaryotic cell, the cell can catalyze specific elongation factor EF2 amide His699 of diphtheria ADP2 ribosylation occurs, thereby preventing protein synthesis and causes cell death. It was designed for treatment of Experimental autoimmune encephalomyelitis (EAE).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7615/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human stomach
Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7615/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7615/28557_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7615/28557_A_9_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7615/28557_A_7_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7615/28557_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Elongated or late spermatids Staining: Medium Intensity: Moderate Quantity:>75% Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Preleptotene spermatocytes Staining: Low Intensity: Moderate Quantity: <25% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7615/28557_A_6_6.jpg
Normal Tissue
Figure 8 Appendix
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Lymphoid tissue Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7615/28557_A_3_2.jpg
Normal Tissue
Figure 9 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7615/28557_A_9_8.jpg
RNA Expression
Figure 10 RNA cell line category: Cell line enhanced (HDLM-2, HHSteC, U-937)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000115604-IL18R1
❮
❯
Specifications
- Host Species
- Mouse
- Species Reactivity
- Mouse
- Molecule Class
- IL-18-DT
- Toxin Description
- Diphtheria toxin (Diphtheriatoxin, DT) is a cell toxin produced by diphtheria, is a protein toxin most commonly used in the construction of recombinant immunotoxin. Before the N-terminal 193 amino acids corresponding to the catalytic domain of DT enzyme, -II binding and elongation factor into the eukaryotic cell, the cell can catalyze specific elongation factor EF2 amide His699 of diphtheria ADP2 ribosylation occurs, thereby preventing protein synthesis and causes cell death.
- Applications
- This product is suitable for use in cytotoxicity assay, functional assay and immunotherapy.
- Related Disease
- Experimental autoimmune encephalomyelitis (EAE)
Target
- Alternative Names
- IL18R1; interleukin 18 receptor 1; Il1rrp; Il18ralpha; interleukin-18 receptor 1; IL-18R1; IL-1Rrp; IL1R-rp; IL-18R-1; IL1 receptor-related protein; CD218 antigen-like family member A; interleukin 1 receptor related protein; Diphtheria toxin; Diphtheria-toxin; DT; DT388; DT389; DT390; DAB422
- Gene ID
- 16182
- UniProt ID
- Q8C257
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for AGTO-G042D. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Related Signaling Pathways
TNF Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "IL18R1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1369z | Mouse Anti-IL18R1 Recombinant Antibody (clone 22B12) | ELISA, WB, IP, FuncS | Mouse IgG1 |
| MOB-0235CT | Recombinant Mouse anti-Human IL18R1 Monoclonal antibody (80736.222) | I-ELISA, Neut, WB | |
| HPAB-658-FY | Mouse Anti-IL18R1 Recombinant Antibody (HPAB-658-FY) | FC | Mouse IgG |
| HPAB-659-FY | Mouse Anti-IL18R1 Recombinant Antibody (HPAB-659-FY) | FC | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-G042E | IL-18-PE immunotoxin | Cytotoxicity assay, Function study |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-207CT | Mouse Anti-IL18R1 Recombinant Antibody (TAB-207CT) | Neut, ELISA, Block | Mouse IgG |
| TAB-207CT-F(E) | Mouse Anti-IL18R1 Recombinant Antibody; Fab Fragment (TAB-207CT-F(E)) | Neut, ELISA, Block | Mouse Fab |
| TAB-208CT-F(E) | Mouse Anti-IL18R1 Recombinant Antibody; Fab Fragment (TAB-208CT-F(E)) | Neut, ELISA, Block | Mouse Fab |
| TAB-209CT-F(E) | Mouse Anti-IL18R1 Recombinant Antibody; Fab Fragment (TAB-209CT-F(E)) | FC, ELISA, Ibhibition | Mouse Fab |
| TAB-210CT-F(E) | Mouse Anti-IL18R1 Recombinant Antibody; Fab Fragment (TAB-210CT-F(E)) | FC, ELISA, Ibhibition | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-211CT | Human Anti-IL18R1 Recombinant Antibody (TAB-211CT) | FC, ELISA, Ibhibition | Humanized antibody |
| TAB-211CT-S(P) | Human Anti-IL18R1 Recombinant Antibody; scFv Fragment (TAB-211CT-S(P)) | FC, ELISA, Ibhibition | Humanized scFv |
| TAB-211CT-F(E) | Human Anti-IL18R1 Recombinant Antibody; Fab Fragment (TAB-211CT-F(E)) | FC, ELISA, Ibhibition | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1247CQ | Mouse Anti-IL18R1 Recombinant Antibody (clone CBL489) | FC, IHC, CyTOF, ICC, Neut | Mouse IgG1 |
| NEUT-1248CQ | Mouse Anti-IL18R1 Recombinant Antibody (clone 70625.111) | I-ELISA, Neut, WB | Mouse IgG1 |
| NEUT-1249CQ | Mouse Anti-IL18R1 Recombinant Antibody (clone 8H22) | IHC, Neut, WB | Mouse IgG1 |
| NEUT-1250CQ | Rat Anti-Il18r1 Recombinant Antibody (clone CBL522) | WB, Neut | Rat IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-4250 | Rabbit Anti-Il18r1 Recombinant Antibody (clone SI329DS) | ELISA | Rabbit IgG |
| MOR-4251 | Rabbit Anti-IL18R1 Recombinant Antibody (clone SI330DS) | ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-658-FY-S(P) | Mouse Anti-IL18R1 Recombinant Antibody; scFv Fragment (HPAB-658-FY-S(P)) | Inhib, FC, ELISA | Mouse scFv |
| HPAB-659-FY-S(P) | Mouse Anti-IL18R1 Recombinant Antibody; scFv Fragment (HPAB-659-FY-S(P)) | ELISA | Mouse scFv |
| FAMAB-0315JF-S(P) | Mouse Anti-IL18R1 Recombinant Antibody (clone 117-10C); scFv Fragment | ELISA, FuncS | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-658-FY-F(E) | Mouse Anti-IL18R1 Recombinant Antibody; Fab Fragment (HPAB-658-FY-F(E)) | Inhib, FC, ELISA | Mouse Fab |
| HPAB-659-FY-F(E) | Mouse Anti-IL18R1 Recombinant Antibody; Fab Fragment (HPAB-659-FY-F(E)) | ELISA | Mouse Fab |
| FAMAB-0315JF-F(E) | Mouse Anti-IL18R1 Recombinant Antibody (clone 117-10C); Fab Fragment | ELISA, FuncS | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0315JF | Mouse Anti-IL18R1 Recombinant Antibody (clone 117-10C) | ELISA, FuncS | Mouse IgM, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-FY4 | Mouse Anti-IL18R1 scFv-Fc Chimera (VS-0425-FY4) | Inhib, ELISA | Mouse IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC211 | Recombinant Anti-IL18R1 Vesicular Antibody, EV Displayed (VS-0425-YC211) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3501 | Anti-IL18R1 Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: WB, IF, IP, Neut, FuncS, ELISA, FC
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
Application: WB, FC, IP, ELISA, Neut, FuncS, IF
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: FuncS, IF, Neut, ELISA, FC, IP, ICC
Application: Neut, ELISA, Inhib, ICC, WB
-3.jpg)
Application: ELISA, FC, IF, IHC, WB
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
