Mouse Anti-PDGFB Recombinant Antibody (clone 29B7)

Name: Mouse Anti-PDGFB Recombinant Antibody (clone 29B7)
SKU: MOB-3853z
Rating: 5 (1 reviews)

CAT#: MOB-3853z

This product is a mouse antibody that recognizes human PDGFB. It can be used in the following applications: WB, IP, IF, ELISA.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line MCF7

Immunofluorescent staining of human cell line MCF7 shows localization to vesicles.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11972/1592_D1_1_selected.jpg

Normal Tissue Figure 2 IHC staining of human caudate

Immunohistochemical staining of human caudate shows strong cytoplasmic positivity in granular pattern in neuronal cells.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11972/ihc_selected.jpg

Normal Tissue Figure 3 Placenta

Cytotrophoblasts Staining: Medium Intensity: Moderate Quantity:>75% Endothelial cells Staining: High Intensity: Strong Quantity:>75% Hofbauer cells Staining: Medium Intensity: Moderate Quantity:>75% Syncytiotrophoblasts - cell body Staining: High Intensity: Strong Quantity:>75% Syncytiotrophoblasts - microvilli Staining: High Intensity: Strong

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11972/28202_A_1_7.jpg

Normal Tissue Figure 4 Lymph node

Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11972/28202_A_9_8.jpg

Normal Tissue Figure 5 Liver

Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11972/28202_A_9_4.jpg

Normal Tissue Figure 6 Kidney

Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11972/28202_A_8_5.jpg

Normal Tissue Figure 7 Testis

Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75%

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18341/40769_A_5_6.jpg

RNA Expression Figure 8 RNA cell line category: Cell line enhanced (A-431, EFO-21, HUVEC TERT2, SK-BR-3, TIME)

Cell lines ordered by descending RNA expression order

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000100311-PDGFB

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Specifications

Immunogen
Human Platelet-Derived Growth Factor Beta Polypeptide

Host Species
Mouse

Derivation
Mouse

Type
Mouse IgG2a

Antibody Isotype
IgG2a

Specificity
Human PDGFB

Species Reactivity
Human

Clone
29B7

Applications
WB, IP, IF, ELISA

Product Property

Purity
>95% as determined by SDS-PAGE

Concentration
Please refer to the vial label for the specific concentration.

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
This antibody has been reported for use in Western Blot, Immunoprecipitation Assay, Immunofluorescence Assay, Enzyme-linked Immunosorbent Assay.

Target

Alternative Names
PDGFB; IBGC5; Platelet-Derived Growth Factor Beta Polypeptide; Platelet-Derived Growth Factor B Chain; Platelet-Derived Growth Factor 2; PDGF-2; Platelet-Derived Growth Factor, Beta Polypeptide (Oncogene SIS); SSV; PDGF, B Chain; PDGF2; Oncogene SIS

UniProt ID
P01127

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - MOB-3853z.pdf

MSDS

MSDS - MOB-3853z.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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