Recombinant Anti-human IGFBP7 Antibody
CAT#: MOB-546
Recombinant Mouse Antibody is specific to Human IGFBP7, expressed in Chinese Hamster Ovary cells(CHO).
Tested Data
Gene Expression
SDS-PAGE
Figure 1 Recombinant Anti-human IGFBP7 Antibody (MOB-546) in SDS-PAGE
Lane 1: Non-reduced mAb
Lane 2: Reduced mAb
SEC-HPLC
Figure 2 Recombinant Anti-human IGFBP7 Antibody (MOB-546) in HPLC
The purity of MOB-546 was greater than 94% as determined by SEC-HPLC.
WB
Figure 3 Recombinant Anti-human IGFBP7 Antibody (MOB-546) in WB
Western blot analysis of MOB-546 was performed with Recombinant Human IGFBP7 Protein (His Tag).
MOB-546 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Anti-Mouse IgG (H+L)
Lane 1: Reducing antigen (0.3μg)
Lane 2: Reducing antigen (0.6μg)
ELISA
Figure 4 Recombinant Anti-human IGFBP7 Antibody (MOB-546) in ELISA
ELISA analysis of MOB-546 was performed with Recombinant Human IGFBP7 Protein (His Tag).
The secondary antibody: HRP-Anti-Mouse IgG (H+L)
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Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20668/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human kidney
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2196/ihc_selected.jpg
Normal Tissue
Figure 3 IHC staining of human pancreas
Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in islet cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20668/ihc_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuronal cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2196/106599_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2196/106599_A_8_3.jpg
Normal Tissue
Figure 6 Liver
Hepatocytes
Staining: Not detected
Intensity:Weak
Quantity: <25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2196/106599_A_8_4.jpg
Normal Tissue
Figure 7 Testis
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2196/106599_A_5_6.jpg
Normal Tissue
Figure 8 Lymph node
Non-germinal center cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2196/106599_A_8_8.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (BJ, BJ hTERT+, hTERT-RPE1, RPTEC TERT1, TIME)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000163453-IGFBP7
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Specifications
- Immunogen
- Human insulin-like growth factor binding protein 7
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- IgG
- Specificity
- Tested positive against native Human insulin-like growth factor binding protein 7
- Species Reactivity
- Human
- Applications
- Products can be used for: Enzyme-linked Immunosorbent Assay; Western blot; Immunofluorescence; Functional Study
Product Property
- Purity
- >98% by SDS-PAGE and HPLC analysis
- Storage
- Short term: store at 4°C (over 6 months), long term: -20°C or -80°C.
Target
- Alternative Names
- IGFBP7; insulin-like growth factor binding protein 7; AGM; PSF; TAF; FSTL2; IBP-7; MAC25; IGFBP-7; RAMSVPS; IGFBP-7v; IGFBPRP1; insulin-like growth factor-binding protein 7; IGFBP-rP1; angiomodulin; IGF-binding protein 7; PGI2-stimulating factor; tumor-de
- Gene ID
- 3490
- UniProt ID
- Q16270
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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|---|---|---|---|
| MOB-546-F(E) | Recombinant Anti-human IGFBP7 Antibody Fab Fragment | WB, FACS, FuncS | Fab |
| MHH-546-F(E) | Recombinant Human Anti-human IGFBP7 Antibody Fab Fragment | ELISA, Nert, FuncS | Fab |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-546-S(P) | Recombinant Anti-human IGFBP7 Antibody scFv Fragment | WB, ELISA, IHC, FuncS | scFv |
| MHH-546-S(P) | Recombinant Human Anti-human IGFBP7 Antibody scFv Fragment | ELISA, WB, FC, FuncS | scFv |
| HPAB-0134-CN-S(P) | Mouse Anti-IGFBP7 Recombinant Antibody; scFv Fragment (HPAB-0134-CN-S(P)) | ELISA | Mouse scFv |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHH-546 | Recombinant Human Anti-human IGFBP7 Antibody | WB, IHC, FuncS | IgG |
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| CAT | Product Name | Application | Type |
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| MOR-1762 | Rabbit Anti-IGFBP7 Recombinant Antibody (clone DS1762AB) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| VS-0525-XY3448 | Anti-Human IGFBP7 Immunohistochemistry Kit | IHC | |
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