Recombinant Human Anti-Gliadin Antibody

CAT#: MHH-397

Recombinant Human Antibody is capable of binding to Gliadin, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-Gliadin mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-Gliadin mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition.

Specifications

  • Immunogen
  • The details of the immunogen for this antibody are not available.
  • Host Species
  • Human
  • Derivation
  • Human
  • Type
  • IgG
  • Applications
  • Antibody assay: Enzyme-linked Immunosorbent Assay; Immunofluorescence; Functional Study

Product Property

  • Purity
  • >97%, by SDS-PAGE under reducing conditions
  • Storage
  • Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.

Target

  • Alternative Names
  • Gliadin; Gliadins
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Breast cancer biomarkers at key points during disease progression
Excellent for Gliadin Detection
I used the Recombinant Human Anti-Gliadin Antibody in ELISA assays to detect gliadin levels in wheat samples. The antibody performed exceptionally well, providing consistent and accurate results. Its high specificity minimized cross-reactivity, which was crucial for my research on gluten content in cereals. The clear instructions made it easy to use, and it has become an essential tool in my laboratory.
Breast cancer biomarkers at key points during disease progression
Valuable for Celiac Disease Research
As a researcher focusing on celiac disease mechanisms, this antibody has been incredibly useful. It allows me to study gliadin interactions with immune cells in vitro. The antibody works well in various assays, including ELISA and functional studies. Its performance has contributed significantly to advancing my research objectives.
Breast cancer biomarkers at key points during disease progression
User-Friendly and Effective
The Recombinant Human Anti-Gliadin Antibody is easy to handle, even for those new to antibody-based techniques. The product came with clear instructions and helpful tips for different applications. When I had questions about optimizing my ELISA protocol, the technical support team was responsive and informative. Overall, it's an effective and user-friendly product.

Q&As

  1. What is the main target of the Recombinant Human Anti-Gliadin Antibody?

    A: The main target of this antibody is gliadin, a protein component of gluten found in wheat and other cereals like barley and rye. Gliadin is known to induce immune responses in certain individuals. This antibody specifically binds to gliadin, making it a valuable tool for research on gluten-related proteins and studying conditions like celiac disease in a laboratory setting.

  2. Can this antibody be used in ELISA assays?

    A: Yes, the Recombinant Human Anti-Gliadin Antibody is well-suited for ELISA applications. It can detect and quantify gliadin in various samples, aiding researchers in studying gluten content and interactions in food products or experimental models.

  3. Can this antibody aid in research on gluten-related disorders?

    A: Yes, this antibody is an excellent tool for investigating gluten-related disorders such as celiac disease at the molecular level in research settings. It helps researchers study gliadin interactions and immune responses in vitro, contributing to a better understanding of these conditions.

View the frequently asked questions answered by Creative Biolabs Support.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# MHH-397, RRID: AB_3111453)

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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Protocol & Troubleshooting

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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