Mouse Anti-FCGR3A Recombinant Antibody (clone 3G8); Fab Fragment (CAT#: DrMAB-979)

Recombinant Mouse Antibody binds selectively to Mouse FCGR3A.


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FC

Figure 1 Transfection/selection procedure for T cells and CD16 expression after transfection.

Figure 1 Transfection/selection procedure for T cells and CD16 expression after transfection.

The windows in the dot plots spot the different lymphocytes subsets.

Ollier, J., Vivien, R., Vié, H., & Clémenceau, B. (2017). Transfection of FcγRIIIa (CD16) Alone Can Be Sufficient To Enable Human αβTCR T Lymphocytes To Mediate Antibody-Dependent Cellular Cytotoxicity. ImmunoHorizons, 1(5), 63-70.

ELISA

Figure 2 Shows results from an ELISA for binding of sCD16A by CD16A binding proteins.

Figure 2 Shows results from an ELISA for binding of sCD16A by CD16A binding proteins.

FC

Figure 3 Shows results of a cell based competition assay.

Figure 3 Shows results of a cell based competition assay.

The aglycosylated humanized antibodies shown compete with aglycosylated chimeric antibody for binding to CHO-K1 cells expressing the extracellular domain of CD16A.

Inhib

Figure 4 Shows inhibition of binding of sCD16A to immune complexes.

Figure 4 Shows inhibition of binding of sCD16A to immune complexes.

Hu3G8-1.1 is an antibody with the heavy chain Hu3G8VH-1, and the light chain Hu3G8VL-1.

Inhib

Figure 5 Shows ITP protection in mice injected i.v. with mAb 3G8 (0.5 μg/g) or human IVIG (1 mg/g) one hour before ch6A6 i.p. injection.

Figure 5 Shows ITP protection in mice injected i.v. with mAb 3G8 (0.5 μg/g) or human IVIG (1 mg/g) one hour before ch6A6 i.p. injection.

ADCC

Figure 6 Shows inhibition of ch4D5 mediated antibody-dependent cell-mediated cytotoxicity (ADCC) by humanized 3G8 antibodies.

Figure 6 Shows inhibition of ch4D5 mediated antibody-dependent cell-mediated cytotoxicity (ADCC) by humanized 3G8 antibodies.

ADCC

Figure 7 Show inhibition of ch4-4-20 mediated ADCC by humanized 3G8 antibodies.

Figure 7 Show inhibition of ch4-4-20 mediated ADCC by humanized 3G8 antibodies.


Specifications

  • Immunogen
  • Human polymorphonuclear leukocytes
  • Host Species
  • Mouse
  • Type
  • Mouse Fab
  • Specificity
  • Human FCGR3A
  • Species Reactivity
  • Human, Monkey
  • Clone
  • 3G8
  • Applications
  • FC, ELISA, Inhib, ADCC

Product Property

  • Purification
  • Purified with Protein A affinity chromatography
  • Purity
  • >95% as determined by SDS-PAGE
  • Concentration
  • 1 mg/mL (lot specific)
  • Buffer
  • 50 mM Sodium Phosphate, 100 mM Potassium Chloride, 150mM NaCl, pH 7.5
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • The FCGR3A antibody has been reported in applications of Flow Cytometry, Enzyme-linked Immunosorbent Assay, Inhibition, Antibody-dependent Cell-mediated Cytotoxicity.

Target

  • Alternative Names
  • FCGR3A; Fc fragment of IgG, low affinity IIIa, receptor (CD16a); Fc fragment of IgG, low affinity IIIa, receptor for (CD16), FCG3, FCGR3; low affinity immunoglobulin gamma Fc region receptor III-A; CD16; CD16a; FcgammaRIIIA; CD16a antigen; fc-gamma RIII;

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "Clone 3G8"

See other products for "FCGR3A"

Single-domain Antibody

CAT Product Name Application Type
NABG-063 Recombinant Anti-Human FCGR3A VHH Single Domain Antibody WB, ELISA, IHC, FuncS Llama VHH
HPAB-1025WJ Recombinant Camelid Anti-FCGR3A Single Domain Antibody ELISA Camelid VHH

Humanized Antibody

Chimeric Antibody

CAT Product Name Application Type
TAB-041WM Anti-Human FCGR3A Recombinant Antibody (ch3G8) ELISA Chimeric Antibody (human/mouse)
TAB-041WM-S(P) Anti-Human FCGR3A Recombinant Antibody scFv Fragment (ch3G8) ELISA Chimeric Antibody (human/mouse)
TAB-041WM-F(E) Anti-Human FCGR3A Recombinant Antibody Fab Fragment (ch3G8) ELISA Chimeric Antibody (human/mouse)

Mouse Antibody

CAT Product Name Application Type
MOB-0275MC Recombinant Mouse Anti-Human FCGR3A Antibody. FC, IP, IHC

Blocking Antibody

CAT Product Name Application Type
NEUT-797CQ Mouse Anti-FCGR3A Recombinant Antibody (clone 3G8) FC, IHC-Fr, ICC, IP, Stim, Costim, Block Mouse IgG1, κ

Neutralizing Antibody

CAT Product Name Application Type
NEUT-798CQ Mouse Anti-FCGR3A Recombinant Antibody (clone B73.1) FC, FuncS, Neut Mouse IgG1, κ

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-1275 Hi-Affi™ Rabbit Anti-FCGR3A Recombinant Antibody (clone DS1275AB) WB, ICC Rabbit IgG

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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