Recombinant Mouse Anti-Lysozyme Antibody Fab Fragment (HyHEL-5)

Figure 1 Detection of lysozyme in HEL/CP4 and VP2 capsids by slot blot.

In protein slot blot for HEL and VP2, proteins were detected by application of mouse mAbs directed against B19 capsid protein, conformationally determined epitopes of HEL (HyHEL-5, -10, -15) and, as a control, anti-CD3 antibody. Anti-HEL antibodies bound only to chimeric capsids.

Miyamura, K., Kajigaya, S., Momoeda, M., Smith-Gill, S. J., & Young, N. S. (1994). Parvovirus particles as platforms for protein presentation. Proceedings of the National Academy of Sciences, 91(18), 8507-8511.

Figure 2 Superposition of the antibody–lysozyme complexes for conventional antibodies (Left) and for VHHs (Right).

The HEWL molecules (gray surfaces) are shown in the same orientation for both antibody classes. The antibody molecules are represented as colored ribbons and their identity is indicated in the same color.

De Genst, E., Silence, K., Decanniere, K., Conrath, K., Loris, R., Kinne, J., ... & Wyns, L. (2006). Molecular basis for the preferential cleft recognition by dromedary heavy-chain antibodies. Proceedings of the National Academy of Sciences, 103(12), 4586-4591.