Mouse Anti-ACLY Recombinant Antibody (clone 5F8D11)
CAT#: VS3-QX6
This product is a mouse antibody that recognizes ACLY. The antibody can be used in applications: WB, IHC, ICC, FC, ELISA.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28758/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human kidney
Immunohistochemical staining of human kidney shows distinct nuclear and cytoplasmic positivity in cells in tubules.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7783/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/22434/395_D3_1_blue_red_green.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/22434/399_D3_1_blue_red_green.jpg
Normal Tissue
Figure 5 Colon
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/22959/49962_A_9_3.jpg
Normal Tissue
Figure 6 Pancreas
Pancreatic endocrine cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/22959/49962_A_3_3.jpg
Normal Tissue
Figure 7 Kidney
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/22959/49962_A_7_5.jpg
Normal Tissue
Figure 8 Testis
Cells in seminiferous ducts
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous nuclear
Leydig cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/22959/49962_A_6_6.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000131473-ACLY
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Specifications
- Immunogen
- Purified recombinant fragment of human ACLY (aa 306-502) expressed in E. coli.
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG1
- Specificity
- ACLY
- Species Reactivity
- Human, Mouse, Monkey, Rat
- Clone
- 5F8D11
- Applications
- WB, IHC, ICC, FC, ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Store at 4°C for short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1:500-1:2000; IHC: 1:200-1:1000; ICC: 1:50; FC: 1:200-1:400; ELISA: 1:10000
Target
REVIEWS AND Q&AS
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Strong Western Blot Signal
Using clone 5F8D11, we achieved excellent Western blot results detecting ACLY. The antibody recognized the recombinant fragment of human ACLY expressed in E. coli. We followed the suggested dilution of 1:500-1:2000, which produced sharp bands for samples derived from both human and mouse tissues.
Versatile For Multiple Species
This recombinant antibody is highly versatile, showing reactivity across human, mouse, monkey, and rat samples. We tested it in immunohistochemistry at a 1:200 dilution and obtained distinct cytoplasmic staining consistent with ATP citrate lyase distribution. The high purity (>95%) ensures minimal background.
Q&As
-
What immunogen was used to generate this antibody?
A: The antibody was generated using a purified recombinant fragment of human ACLY (amino acids 306-502) expressed in E. coli. It is a mouse monoclonal antibody (clone 5F8D11) that recognizes ACLY from human, mouse, monkey, and rat sources.
-
What are the recommended dilutions for WB and IHC?
A: We recommend a dilution range of 1:500-1:2000 for Western Blot (WB) and 1:200-1:1000 for Immunohistochemistry (IHC). For Immunocytochemistry (ICC), a dilution of 1:50 is suggested, and for Flow Cytometry (FC), 1:200-1:400 is recommended.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "ACLY"
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|---|---|---|---|
| NAB-17-VHH | Recombinant Anti-human ACLY VHH Single Domain Antibody | IHC, IP, FC, Neut, FUNC | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0038 | Hi-Affi™ Rabbit Anti-ACLY Recombinant Antibody (clone DS38AB) | WB, FC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0140-CN | Rabbit Anti-ACLY Recombinant Antibody (clone CBACN-054) | WB, IF, IHC, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1666-CN | Rabbit Anti-ACLY Polyclonal Antibody (MRO-1666-CN) | WB, IF, IHC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0526J | Rabbit Anti-ACLY Recombinant Antibody (clone 3A5) | ELISA, WB, IF, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-WK681 | Rabbit Anti-ACLY Recombinant Antibody (clone JU80-30) | WB, ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| VS3-CJ98 | Mouse Anti-ACLY Recombinant Antibody (VS3-CJ98) | WB, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
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| VS-0724-YC691 | AbPlus™ Anti-ACLY Magnetic Beads (VS-0724-YC691) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY21 | Anti-ACLY Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC17 | CytoStream™ Mouse Anti-ACLY Recombinant Antibody (VS13-YC17) | WB, IF, FC | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY78 | Anti-Mouse ACLY Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY77 | Anti-Human ACLY Immunohistochemistry Kit | IHC |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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