Mouse Anti-ACLY Recombinant Antibody (VS3-CJ98) (CAT#: VS3-CJ98)

• Immunogen
• Recombinant fragment of human ACLY (C-terminus)

• Host Species
• Mouse

• Type
• Mouse IgG

• Specificity
• Human ACLY

• Species Reactivity
• Human

• Applications
• WB, ELISA

• Conjugate
• Unconjugated

• Purification
• Protein G affinity purified

• Purity
• >95% as determined by SDS-PAGE

• Format
• Liquid

• Concentration
• 1 mg/mL (lot specific)

• Buffer
• 50% Glycerol, 1% BSA, PBS, pH7.4.

• Preservative
• 0.02% sodium azide

• Storage
• Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• This antibody has been tested for use in ELISA (1:5000-1:20000), Western Blot (1:100-1:2000).

• Alternative Names
• ACL; ATPCL; CLATP

• Gene ID
• 47

• UniProt ID
• P53396

• Sequence Similarities
• In the N-terminal section; belongs to the succinate/malate CoA ligase beta subunit family.
  In the C-terminal section; belongs to the succinate/malate CoA ligase alpha subunit family.

• Cellular Localization
• Cytoplasm

• Post Translation Modifications
• Phosphorylated by PKA and GSK3 in a sequential manner; phosphorylation results in activation of its activity (PubMed:10653665).
  Phosphorylation on Thr-447 and Ser-451 depends on the phosphorylation state of Ser-455 (By similarity).
  Phosphorylation on Ser-455 is decreased by prior phosphorylation on the other 2 residues (By similarity)
  ISGylated.
  Acetylated at Lys-540, Lys-546 and Lys-554 by KAT2B/PCAF (PubMed:23932781).
  Acetylation is promoted by glucose and stabilizes the protein, probably by preventing ubiquitination at the same sites (PubMed:23932781).
  Acetylation promotes de novo lipid synthesis (PubMed:23932781).
  Deacetylated by SIRT2.
  Ubiquitinated at Lys-540, Lys-546 and Lys-554 by the BCR(KLHL25) E3 ubiquitin ligase complex and UBR4, leading to its degradation (PubMed:23932781, PubMed:27664236, PubMed:34491895).
  Ubiquitination is probably inhibited by acetylation at same site (PubMed:23932781).
  BCR(KLHL25)-mediated degradation of ACLY promotes fatty acid oxidation and is required for differentiation of inducible regulatory T (iTreg) cells (PubMed:34491895).

• Protein Refseq
• NP_001087.2; NP_001290203.1; NP_001290204.1

• Function
• Catalyzes the cleavage of citrate into oxaloacetate and acetyl-CoA, the latter serving as common substrate for de novo cholesterol and fatty acid synthesis.

Isotype Control

• MRO-A1591-YJ
• Mouse IgG Isotype control, Polyclonal

Secondary Antibody

• 2ND-040CQ
• Goat Anti-Mouse IgG (H&L) Antibody

• 2ND-042CQ
• Rabbit Anti-Mouse IgG (H&L) Antibody

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS

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