Mouse Anti-ACTB Recombinant Antibody (clone 3B12-B7-B7)
CAT#: VS3-QX19
This product is a mouse antibody that recognizes ACTB. The antibody can be used in applications: WB, ICC, IHC, FC.
Gene Expression
Normal Tissue
Figure 1 IHC staining of human tonsil
Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in reaction center cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2621/ihc_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41271/103139_B_7_5.jpg
Normal Tissue
Figure 3 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2621/6575_A_6_6.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41271/103139_A_7_8.jpg
Normal Tissue
Figure 5 Colon
Enterocytes - Microvilli Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41271/103139_A_8_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41271/103139_A_9_5.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000075624-ACTB
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Specifications
- Immunogen
- ACTB recombinant protein.
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG
- Specificity
- β-Actin
- Species Reactivity
- Human, Mouse, Rat
- Clone
- 3B12-B7-B7
- Applications
- WB, ICC, IHC, FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.09% sodium azide
- Storage
- Store at 4°C for short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1:1000; ICC: 1:10-1:50; IHC: 1:25; FC: 1:25
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "ACTB"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABL-055 | Recombinant Anti-human ACTB VHH Single Domain Antibody | WB, ELISA, IP, FC, FuncS | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0345MC | Recombinant Human Anti-Actin Beta Antibody Fab Fragment | WB |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0017MZ | Chicken Anti-Actin, Cytoplasmic 1 Polyclonal IgY | WB | Chicken antibody |
| BRD-0018MZ | Chicken Anti-Actin, Non-muscle (β and γ) Polyclonal IgY | WB | Chicken antibody |
| BRD-0687MZ | Chicken Anti-Beta actin Polyclonal IgY | ICC, IF, WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0049 | Hi-Affi™ Rabbit Anti-ACTB Recombinant Antibody (clone DS49AB) | IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0283U | Mouse Anti-ACTB Recombinant Antibody (ZG-0283U) | ELISA | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0296U | Mouse Anti-ACTB Recombinant Antibody (clone 1D12E12B5) | ELISA, WB, IHC, IF, FC, IP | Mouse IgG2b |
| VS3-QX14 | Mouse Anti-ACTB Recombinant Antibody (clone 6D7-F6-G10) | WB | Mouse IgG3 |
| VS3-QX15 | Mouse Anti-ACTB Recombinant Antibody (clone 5C1-4B9-8C7) | WB | Mouse IgG1 |
| VS-0923-FY1 | Recombinant Mouse Anti-ACTB Antibody (VS-0923-FY1) | ELISA, WB | Mouse IgG1 |
| VS-0923-FY2 | Recombinant Mouse Anti-ACTB Antibody (VS-0923-FY2) | ELISA, WB, IF | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1253 | AbPlus™ Anti-ACTB Magnetic Beads (VS-0724-YC1253) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY53 | Mouse Anti-NHP ACTB Recombinant Antibody (VS-1024-XY53) | WB, IHC | Mouse IgG2b, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY28 | Anti-ACTB Immunohistochemistry Kit | IHC | |
| VS-0525-XY114 | Anti-Mouse ACTB Immunohistochemistry Kit | IHC | |
| VS-0525-XY113 | Anti-Human ACTB Immunohistochemistry Kit | IHC | |
| VS-0525-XY115 | Anti-Rat ACTB Immunohistochemistry Kit | IHC |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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