Mouse Anti-ALK/p80 Recombinant Antibody (VS3-FY2624)
CAT#: VS3-FY2624
This anti-ALK mAb is a mouse recombinant antibody that is created to recognize ALK/p80 protein, a calculated weight of 176.4 kDa. It exhibits cross-reactivity with human. This monoclonal antibody is potential for conducting research on Neuroblastoma 3 and Neuroblastoma. The antibody can be utilized for detection of ALK by Western Blot, Flow cytometry, ELISA.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line RH-30
Immunofluorescent staining of human cell line RH-30 shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10694/1416_A5_3_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10694/26100_B_8_5.jpg
Normal Tissue
Figure 3 Cerebellum
Cells in molecular layer Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear Purkinje cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10694/26100_B_7_8.jpg
Normal Tissue
Figure 4 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10694/26075_A_7_3.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10694/26075_A_6_6.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enriched (SH-SY5Y)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000171094-ALK
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- ALK receptor tyrosine kinase of human
- Species Reactivity
- Human
- Applications
- WB, FC, ELISA
- Conjugate
- Unconjugated, FITC
- MW
- The molecular weight of the target protein is approximately 176.4 kDa.
- Related Disease
- Neuroblastoma 3 and Neuroblastoma
Product Property
- Clonality
- Monoclonal Antibody
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/2000
Flow cytometry: 1/50-1/200
ELISA: 1/1000-1/10000
Target
- Alternative Names
- ALK Receptor Tyrosine Kinase; Anaplastic Lymphoma Receptor Tyrosine Kinase; CD246 Antigen; EC 2.7.10.1; Anaplastic Lymphoma Kinase (Ki-1); Mutant Anaplastic Lymphoma Kinase
- Gene ID
- 238
- Sequence Similarities
- Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
- Cellular Localization
- Cell membrane
- Post Translation Modifications
- Phosphorylated at tyrosine residues by autocatalysis, which activates kinase activity.
In cells not stimulated by a ligand, receptor protein tyrosine phosphatase beta and zeta complex dephosphorylates ALK at the sites in ALK that are undergoing autophosphorylation through autoactivation.
Phosphorylation at Tyr-1507 is critical for SHC1 association.
N-glycosylated.
Glycosylation at Asn169, Asn244, Asn285, Asn324, Asn411, Asn424, Asn445, Asn563, Asn571, Asn627, Asn709, Asn808, Asn863, Asn864, Asn886, and Asn986
Modification sites at PhosphoSitePlus
Glycosylation from GlyGen 17 sites, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_054197220.1; NP_001340694.1; NP_004295.2
- Function
- Neuronal receptor tyrosine kinase that is essentially and transiently expressed in specific regions of the central and peripheral nervous systems and plays an important role in the genesis and differentiation of the nervous system (PubMed:11121404, 11387242, 16317043, 17274988, 30061385, 34646012, 34819673).
Also acts as a key thinness protein involved in the resistance to weight gain: in hypothalamic neurons, controls energy expenditure acting as a negative regulator of white adipose tissue lipolysis and sympathetic tone to fine-tune energy homeostasis (By similarity).
Following activation by ALKAL2 ligand at the cell surface, transduces an extracellular signal into an intracellular response.
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Related Diseases
EGFR Tyrosine Kinase Inhibitor Resistance
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "ALK"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0296MZ | Mouse Anti-ALK Recombinant Antibody (clone BLK2) | IHC | Mouse IgG3 |
| HPAB-1817LY | Mouse Anti-ALK Recombinant Antibody (HPAB-1817LY) | ELISA, WB, FC | Mouse IgG |
| HPAB-1818LY | Mouse Anti-ALK Recombinant Antibody (HPAB-1818LY) | ELISA, WB, FC | Mouse IgG |
| VS7-HM85 | Mouse Anti-ALK Recombinant Antibody (clone 8E8D3) | FCM | Mouse IgG1 |
| VS7-HM86 | Mouse Anti-ALK Recombinant Antibody (clone 7A11A4) | ICC, FCM | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0147 | Hi-Affi™ Rabbit Anti-ALK Recombinant Antibody (clone DS147AB) | IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0138-YC-S(P) | Mouse Anti-ALK Recombinant Antibody; scFv Fragment (HPAB-0138-YC-S(P)) | ELISA, FC, WB | Mouse scFv |
| HPAB-0139-YC-S(P) | Mouse Anti-ALK Recombinant Antibody; scFv Fragment (HPAB-0139-YC-S(P)) | ELISA, FC, WB | Mouse scFv |
| HPAB-0140-YC-S(P) | Mouse Anti-ALK Recombinant Antibody; scFv Fragment (HPAB-0140-YC-S(P)) | ELISA, FC, WB | Mouse scFv |
| HPAB-0141-YC-S(P) | Mouse Anti-ALK Recombinant Antibody; scFv Fragment (HPAB-0141-YC-S(P)) | ELISA, FC, RIA | Mouse scFv |
| HPAB-0143-YC-S(P) | Mouse Anti-ALK Recombinant Antibody (clone D10); scFv Fragment | ELISA, FC, Stim | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-004CN | Human Anti-ALK Recombinant Antibody (clone ESBA521) | ELISA, WB, FuncS | Human IgG |
| NS-004CN-F(E) | Human Anti-ALK Recombinant Antibody (clone ESBA521); Fab Fragment | ELISA, WB | Human Fab |
| NS-004CN-S(P) | Human Anti-ALK Recombinant Antibody (clone ESBA521); scFv Fragment | ELISA, WB | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1817LY-F(E) | Mouse Anti-ALK Recombinant Antibody; Fab Fragment (HPAB-1817LY-F(E)) | ELISA, WB, FC | Mouse Fab |
| HPAB-1818LY-F(E) | Mouse Anti-ALK Recombinant Antibody; Fab Fragment (HPAB-1818LY-F(E)) | ELISA, WB, FC | Mouse Fab |
| HPAB-1819LY-F(E) | Mouse Anti-ALK Recombinant Antibody; Fab Fragment (HPAB-1819LY-F(E)) | ELISA, WB, FC | Mouse Fab |
| HPAB-1820LY-F(E) | Mouse Anti-ALK Recombinant Antibody; Fab Fragment (HPAB-1820LY-F(E)) | ELISA, WB, FC | Mouse Fab |
| HPAB-1821LY-F(E) | Mouse Anti-ALK Recombinant Antibody; Fab Fragment (HPAB-1821LY-F(E)) | ELISA, WB, FC | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC625 | Recombinant Anti-ALK Vesicular Antibody, EV Displayed (VS-0425-YC625) | ELISA, FC, Stim, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY319 | Anti-ALK Immunohistochemistry Kit | IHC |
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