Rabbit Anti-ATF2 Recombinant Antibody (clone 3D12)
CAT#: ZG-0552J
This product is a rabbit antibody that recognizes ATF2.
Tested Data
Gene Expression
WB
Figure 1 Rabbit Anti-ATF2 Antibody (ZG-0552J) in WB
Western Blot
Positive WB detected in: K562 whole cell lysate, 293T whole cell lysate
All lanes: ATF2 antibody at 1.2μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 55, 36, 24, 49, 53, 14, 25, 16 KDa
Observed band size: 60 KDa
IHC
Figure 2 Rabbit Anti-ATF2 Antibody (ZG-0552J) in IHC
IHC image of ZG-0552J diluted at 1:115.5 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22134/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3769/if_selected.jpg
Normal Tissue
Figure 3 IHC staining of human prostate
Immunohistochemical staining of human prostate shows moderate nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22134/ihc_selected.jpg
Normal Tissue
Figure 4 IHC staining of human cerebellum
Immunohistochemical staining of human cerebellum shows strong nuclear positivity in Purkinje cells and cells in molecular and granular layer.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3769/11607_B_7_8_selected.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3769/11607_A_8_8.jpg
Normal Tissue
Figure 6 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3769/11607_B_9_5.jpg
Normal Tissue
Figure 7 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3769/11607_A_9_3.jpg
Normal Tissue
Figure 8 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3769/11607_A_9_4.jpg
Normal Tissue
Figure 9 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3769/11607_A_7_5.jpg
Normal Tissue
Figure 10 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3769/11607_A_4_6.jpg
RNA Expression
Figure 11 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000115966-ATF2
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Specifications
- Immunogen
- A synthesized peptide derived from human ATF2
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human ATF2
- Species Reactivity
- Human
- Clone
- 3D12
- Applications
- ELISA, WB, IHC
- Conjugate
- Uconjugated
- Related Disease
- Cancer
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry.
WB:1:500-1:5000, IHC:1:50-1:200
Target
- Alternative Names
- HB16; CREB2; TREB7; CREB-2; CRE-BP1; cyclic AMP-dependent transcription factor ATF-2; activating transcription factor 2 splice variant ATF2-var2; cAMP response element-binding protein CRE-BP1; cAMP responsive element binding protein 2, formerly; cAMP-dependent transcription factor ATF-2; cyclic AMP-responsive element-binding protein 2; histone acetyltransferase ATF2
- Gene ID
- 1386
- UniProt ID
- P15336
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Related Signaling Pathways
BCR Signaling Pathway
Canonical Wnt Signaling Pathway
MAPK Signaling Pathway
Non-Canonical Wnt Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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