Rabbit Anti-ATF2 Recombinant Antibody (clone 3D12) (CAT#: ZG-0552J)

Name: Rabbit Anti-ATF2 Recombinant Antibody (clone 3D12)
SKU: ZG-0552J
Rating: 5 (1 reviews)

This product is a rabbit antibody that recognizes ATF2.

Custom Production

We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

We require custom production

Tested Data
Gene Expression
Datasheet
MSDS
COA

Figure 1 Rabbit Anti-ATF2 Antibody (ZG-0552J) in WB

Figure 1 Rabbit Anti-ATF2 Antibody (ZG-0552J) in WB

Western Blot
Positive WB detected in: K562 whole cell lysate, 293T whole cell lysate
All lanes: ATF2 antibody at 1.2μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 55, 36, 24, 49, 53, 14, 25, 16 KDa
Observed band size: 60 KDa

IHC

Figure 2 Rabbit Anti-ATF2 Antibody (ZG-0552J) in IHC

Figure 2 Rabbit Anti-ATF2 Antibody (ZG-0552J) in IHC

IHC image of ZG-0552J diluted at 1:115.5 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Subcellular Location

Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22134/if_selected.jpg

Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3769/if_selected.jpg

Normal Tissue

Figure 3 IHC staining of human prostate
(Immunohistochemical staining of human prostate shows moderate nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22134/ihc_selected.jpg

Figure 4 IHC staining of human cerebellum
(Immunohistochemical staining of human cerebellum shows strong nuclear positivity in Purkinje cells and cells in molecular and granular layer.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3769/11607_B_7_8_selected.jpg

Figure 5 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3769/11607_A_8_8.jpg

Figure 6 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3769/11607_B_9_5.jpg

Figure 7 Colon
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3769/11607_A_9_3.jpg

Figure 8 Liver
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3769/11607_A_9_4.jpg

Figure 9 Kidney
(Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3769/11607_A_7_5.jpg

Figure 10 Testis
(Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3769/11607_A_4_6.jpg

RNA Expression

Figure 11 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000115966-ATF2

Datasheet

MSDS

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Immunogen
A synthesized peptide derived from human ATF2

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human ATF2

Species Reactivity
Human

Clone
3D12

Applications
ELISA, WB, IHC

Conjugate
Uconjugated

Related Disease
Cancer

Product Property

Purification
Affinity purified

Format
Liquid

Concentration
See the COA

Buffer
PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Preservative
0.02% sodium azide

Storage
Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry.
WB:1:500-1:5000, IHC:1:50-1:200

Target

Alternative Names
HB16; CREB2; TREB7; CREB-2; CRE-BP1; cyclic AMP-dependent transcription factor ATF-2; activating transcription factor 2 splice variant ATF2-var2; cAMP response element-binding protein CRE-BP1; cAMP responsive element binding protein 2, formerly; cAMP-dependent transcription factor ATF-2; cyclic AMP-responsive element-binding protein 2; histone acetyltransferase ATF2

Gene ID
1386

UniProt ID
P15336

Related Resources

Related Signaling Pathways

BCR Signaling Pathway

Canonical Wnt Signaling Pathway

MAPK Signaling Pathway

Non-Canonical Wnt Signaling Pathway

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "ATF2"

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CAT	Product Name	Application	Type
MOB-0673MZ	Mouse Anti-ATF2 Recombinant Antibody (clone CBLS-122)	ELISA	Mouse IgG2a
MRO-0130-CN	Rabbit Anti-ATF2 Recombinant Antibody (clone CBACN-047)	WB, IF, IHC, IP	Rabbit IgG
MRO-2301-CN	Rabbit Anti-ATF2 Recombinant Antibody (clone CBACN-595)	WB, IF, IP	Rabbit IgG
VS3-XY99	Mouse Anti-ATF2 Recombinant Antibody (clone 4F12)	ELISA, WB, IHC	Mouse IgG1
VS7-HM145	Mouse Anti-ATF2 Recombinant Antibody (clone CBL055HM)	WB, IHC-p, IF, ELISA	Mouse IgG

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