Rabbit Anti-BID Recombinant Antibody (clone R08-2F0)
CAT#: VS3-FY1976
This recombinant antibody specifically binds to human BID. It is suitable for use in a variety of assays, including WB, IHC-F, IHC-P, ICC/IF, IP. To validate the QC result of the antibody, we recommend a dilution for the antibody by western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/50-1/100), immunofluorescence (dilution 1/50-1/200), immunoprecipitation (dilution 1/20).
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/722/if_selected.jpg
Normal Tissue
Figure 2 Bone marrow
Hematopoietic cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3771/10351_B_4_4.jpg
Normal Tissue
Figure 3 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3771/10351_A_8_8.jpg
Normal Tissue
Figure 4 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3771/10351_A_9_3.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (MCF7)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000015475-BID
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ICC
Figure 1 Anti-BID Antibody (VS3-FY1976) in ICC.
Immunocytochemistry staining of Bid (green) in Hela using the anti-Bid antibody. Nuclear DNA was labeled with DAPI (shown in blue).
WB
Figure 2 Anti-BID Antibody (VS3-FY1976) in WB.
The Bid expression in Hela, A549, HL-60 lysates were detected using the anti-Bid by western blot assay.
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Specifications
- Immunogen
- Synthetic peptide of human Bid
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human BID
- Species Reactivity
- Human
- Clone
- R08-2F0
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 22 kDa, while the observed molecular weight stands at 22 kDa.
- Related Disease
- Colon Adenocarcinoma and Prostate Cancer
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- BH3 Interacting Domain Death Agonist; P22 BID; BH3-Interacting Domain Death Agonist; Human BID Coding Sequence; Apoptic Death Agonist; BID Isoform ES(1b)
- Gene ID
- 637
- UniProt ID
- P55957
- Cellular Localization
- Cytoplasm, Membrane, Mitochondrion, Mitochondrion outer membrane
- Post Translation Modifications
- [BH3-interacting domain death agonist]: TNF-alpha induces caspase-mediated cleavage into a major p15 and minor p13 and p11 products ().
Cleaved by CASP6 into a major p15 and minor p13 products, leading to release of cytochrome c and subsequent nonalcoholic steatohepatitis (PubMed:32029622).
(P55957-BID_HUMAN)
[BH3-interacting domain death agonist p15]: Ubiquitinated by ITCH; ubiquitination results in proteasome-dependent degradation. (P55957-BID_HUMAN)
Ubiquitination at Lys158 (NX_P55957 [NX_P55957-1])
Modification sites at PhosphoSitePlus (P55957)
Glycosylation from GlyGen (P55957) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_001187.1; NP_001231496.1; NP_001231498.1; NP_001231499.1; NP_001231501.1
- Function
- Induces caspases and apoptosis.
Counters the protective effect of BCL2 (By similarity).
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Related Signaling Pathways
p53 Signaling Pathway
Related Diseases
Amyotrophic Lateral Sclerosis
Maturity Onset Diabetes of the Young
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "BID"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2323z | Mouse Anti-BID Recombinant Antibody (clone 19H5) | WB, ELISA, FC, IF, IHC | Mouse IgG1 |
| MOB-2266MZ | Mouse Anti-BID Recombinant Antibody (clone 4D6) | ELISA, FC, IF, IHC-P, WB | Mouse IgG1 |
| VS3-CJ1050 | Mouse Anti-BID Recombinant Antibody (VS3-CJ1050) | WB, ICC, IHC | Mouse IgG1, κ |
| VS7-HM229 | Mouse Anti-BID Recombinant Antibody (clone 3C5) | WB, IHC, ICC, FCM | Mouse IgG1 |
| VS7-HM230 | Mouse Anti-BID Recombinant Antibody (clone CBL072HM) | WB, IHC-p, IF, ICC, FCM, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0356 | Hi-Affi™ Rabbit Anti-BID Recombinant Antibody (clone DS356AB) | FC, IF, IHC-P, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-XY4 | Rat Anti-BID Agonistic Antibody (VS-0724-XY4) | WB | Rat IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC630 | AbPlus™ Anti-BID Magnetic Beads (VS-0724-YC630) | IP, Protein Purification | |
| VS-0724-YC751 | AbPlus™ Anti-Bid Magnetic Beads (VS-0724-YC751) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY243 | Anti-BID Immunohistochemistry Kit | IHC | |
| VS-0525-XY768 | Anti-Human BID Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC100 | CytoStream™ Rabbit Anti-BID Recombinant Antibody (VS13-YC100) | WB, ICC, IF, IHC-P, FC, IP | Rabbit IgG |
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