Rabbit Anti-BID Recombinant Antibody (clone R08-2F0) (CAT#: VS3-FY1976)
This recombinant antibody specifically binds to human BID. It is suitable for use in a variety of assays, including WB, IHC-F, IHC-P, ICC/IF, IP. To validate the QC result of the antibody, we recommend a dilution for the antibody by western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/50-1/100), immunofluorescence (dilution 1/50-1/200), immunoprecipitation (dilution 1/20).
Specific Inquiry
ICC
Figure 1 Anti-BID Antibody (VS3-FY1976) in ICC.
Immunocytochemistry staining of Bid (green) in Hela using the anti-Bid antibody. Nuclear DNA was labeled with DAPI (shown in blue).
WB
Figure 2 Anti-BID Antibody (VS3-FY1976) in WB.
The Bid expression in Hela, A549, HL-60 lysates were detected using the anti-Bid by western blot assay.
Specifications
- Immunogen
- Synthetic peptide of human Bid
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human BID
- Species Reactivity
- Human
- Clone
- R08-2F0
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 22 kDa, while the observed molecular weight stands at 22 kDa.
- Related Disease
- Colon Adenocarcinoma and Prostate Cancer
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- BH3 Interacting Domain Death Agonist; P22 BID; BH3-Interacting Domain Death Agonist; Human BID Coding Sequence; Apoptic Death Agonist; BID Isoform ES(1b)
- Gene ID
- 637
- UniProt ID
- P55957
- Cellular Localization
- Cytoplasm, Membrane, Mitochondrion, Mitochondrion outer membrane
- Post Translation Modifications
- [BH3-interacting domain death agonist]: TNF-alpha induces caspase-mediated cleavage into a major p15 and minor p13 and p11 products ().
Cleaved by CASP6 into a major p15 and minor p13 products, leading to release of cytochrome c and subsequent nonalcoholic steatohepatitis (PubMed:32029622).
(P55957-BID_HUMAN)
[BH3-interacting domain death agonist p15]: Ubiquitinated by ITCH; ubiquitination results in proteasome-dependent degradation. (P55957-BID_HUMAN)
Ubiquitination at Lys158 (NX_P55957 [NX_P55957-1])
Modification sites at PhosphoSitePlus (P55957)
Glycosylation from GlyGen (P55957) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_001187.1; NP_001231496.1; NP_001231498.1; NP_001231499.1; NP_001231501.1
- Function
- Induces caspases and apoptosis.
Counters the protective effect of BCL2 (By similarity).
Recommended Products
Isotype Control
Secondary Antibody
Related Resources
p53 Signaling Pathway
Amyotrophic Lateral Sclerosis
Maturity Onset Diabetes of the Young
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
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Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2323z | Mouse Anti-BID Recombinant Antibody (clone 19H5) | WB, ELISA, FC, IF, IHC | Mouse IgG1 |
| MOB-2266MZ | Mouse Anti-BID Recombinant Antibody (clone 4D6) | ELISA, FC, IF, IHC-P, WB | Mouse IgG1 |
| MRO-0179-CN | Rabbit Anti-BID Recombinant Antibody (clone JM11-14) | WB, IF, IHC, FC, IP | Rabbit IgG |
| VS3-CJ1050 | Mouse Anti-BID Recombinant Antibody (VS3-CJ1050) | WB, ICC, IHC | Mouse IgG1, κ |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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