CytoStream™ Rabbit Anti-BID Recombinant Antibody (VS13-YC100) (CAT#: VS13-YC100)
The CytoStream™ Rabbit Anti-BID Recombinant Antibody is specific for human BID. BID, a BCL-2 family death agonist, forms dimers with apoptotic regulators, facilitating mitochondrial damage via CASP8 cleavage and cytochrome c release, with spliced variants encoding different lengths. The anti-BID antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/722/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/722/if_selected.jpg
Figure 2 Bone marrow
(Hematopoietic cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3771/10351_B_4_4.jpg
(Hematopoietic cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3771/10351_B_4_4.jpg
Figure 3 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3771/10351_A_8_8.jpg
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3771/10351_A_8_8.jpg
Figure 4 Colon
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3771/10351_A_9_3.jpg
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3771/10351_A_9_3.jpg
Figure 5 RNA cell line category: Cell line enhanced (MCF7)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000015475-BID
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000015475-BID
Specifications
- Immunogen
- Recombinant human Bid, aa 40-72.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human BID
- Species Reactivity
- Human
- Applications
- WB, ICC, IF, IHC-P, FC, IP
- Conjugate
- NONE
- Related Disease
- Colon Adenocarcinoma and Prostate Cancer
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/1000
Immunocytochemistry (ICC): 1/50-1/200
Immunofluorescence (IF): 1/50-1/200
Immunohistochemistry-paraffin (IHC-P): 1/50-1/200
Flow cytometry (FC): 1/50-1/100
Immunoprecipitation (IP): 1/10-1/50
Target
- Alternative Names
- BH3 Interacting Domain Death Agonist; P22 BID; BH3-Interacting Domain Death Agonist; Human BID Coding Sequence; Apoptic Death Agonist; BID Isoform ES(1b);
- Gene ID
- 637
- UniProt ID
- P55957
- Long Name
- BH3 Interacting Domain Death Agonist
- Cellular Localization
- Cytoplasm; Cell membrane; Mitochondrion; Mitochondrion outer membrane
- Protein Refseq
- NP_001187.1; NP_001231496.1; NP_001231498.1; NP_001231499.1; NP_001231501.1
- Function
- Induces caspases and apoptosis.
Counters the protective effect of BCL2 (By similarity).
Recommended Products
Secondary Antibody
Isotype Control
Related Resources
p53 Signaling Pathway
Amyotrophic Lateral Sclerosis
Maturity Onset Diabetes of the Young
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2323z | Mouse Anti-BID Recombinant Antibody (clone 19H5) | WB, ELISA, FC, IF, IHC | Mouse IgG1 |
| MOB-2266MZ | Mouse Anti-BID Recombinant Antibody (clone 4D6) | ELISA, FC, IF, IHC-P, WB | Mouse IgG1 |
| MRO-0179-CN | Rabbit Anti-BID Recombinant Antibody (clone JM11-14) | WB, IF, IHC, FC, IP | Rabbit IgG |
| VS3-CJ1050 | Mouse Anti-BID Recombinant Antibody (VS3-CJ1050) | WB, ICC, IHC | Mouse IgG1, κ |
| VS3-FY1976 | Rabbit Anti-BID Recombinant Antibody (clone R08-2F0) | WB, IHC-Fr, IHC-P, IF, IP | Rabbit IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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