CytoStream™ Rabbit Anti-BID Recombinant Antibody (VS13-YC100)
CAT#: VS13-YC100
The CytoStream™ Rabbit Anti-BID Recombinant Antibody is specific for human BID. BID, a BCL-2 family death agonist, forms dimers with apoptotic regulators, facilitating mitochondrial damage via CASP8 cleavage and cytochrome c release, with spliced variants encoding different lengths. The anti-BID antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/722/if_selected.jpg
Normal Tissue
Figure 2 Bone marrow
Hematopoietic cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3771/10351_B_4_4.jpg
Normal Tissue
Figure 3 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3771/10351_A_8_8.jpg
Normal Tissue
Figure 4 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3771/10351_A_9_3.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (MCF7)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000015475-BID
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Specifications
- Immunogen
- Recombinant human Bid, aa 40-72.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human BID
- Species Reactivity
- Human
- Applications
- WB, ICC, IF, IHC-P, FC, IP
- Conjugate
- NONE
- Related Disease
- Colon Adenocarcinoma and Prostate Cancer
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/1000
Immunocytochemistry (ICC): 1/50-1/200
Immunofluorescence (IF): 1/50-1/200
Immunohistochemistry-paraffin (IHC-P): 1/50-1/200
Flow cytometry (FC): 1/50-1/100
Immunoprecipitation (IP): 1/10-1/50
Target
- Alternative Names
- BH3 Interacting Domain Death Agonist; P22 BID; BH3-Interacting Domain Death Agonist; Human BID Coding Sequence; Apoptic Death Agonist; BID Isoform ES(1b);
- Gene ID
- 637
- UniProt ID
- P55957
- Long Name
- BH3 Interacting Domain Death Agonist
- Cellular Localization
- Cytoplasm; Cell membrane; Mitochondrion; Mitochondrion outer membrane
- Protein Refseq
- NP_001187.1; NP_001231496.1; NP_001231498.1; NP_001231499.1; NP_001231501.1
- Function
- Induces caspases and apoptosis.
Counters the protective effect of BCL2 (By similarity).
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Related Signaling Pathways
p53 Signaling Pathway
Related Diseases
Amyotrophic Lateral Sclerosis
Maturity Onset Diabetes of the Young
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "BID"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2323z | Mouse Anti-BID Recombinant Antibody (clone 19H5) | WB, ELISA, FC, IF, IHC | Mouse IgG1 |
| MOB-2266MZ | Mouse Anti-BID Recombinant Antibody (clone 4D6) | ELISA, FC, IF, IHC-P, WB | Mouse IgG1 |
| MRO-0179-CN | Rabbit Anti-BID Recombinant Antibody (clone JM11-14) | WB, IF, IHC, FC, IP | Rabbit IgG |
| VS3-CJ1050 | Mouse Anti-BID Recombinant Antibody (VS3-CJ1050) | WB, ICC, IHC | Mouse IgG1, κ |
| VS3-FY1976 | Rabbit Anti-BID Recombinant Antibody (clone R08-2F0) | WB, IHC-Fr, IHC-P, IF, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0356 | Hi-Affi™ Rabbit Anti-BID Recombinant Antibody (clone DS356AB) | FC, IF, IHC-P, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-XY4 | Rat Anti-BID Agonistic Antibody (VS-0724-XY4) | WB | Rat IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC630 | AbPlus™ Anti-BID Magnetic Beads (VS-0724-YC630) | IP, Protein Purification | |
| VS-0724-YC751 | AbPlus™ Anti-Bid Magnetic Beads (VS-0724-YC751) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY243 | Anti-BID Immunohistochemistry Kit | IHC | |
| VS-0525-XY768 | Anti-Human BID Immunohistochemistry Kit | IHC |
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