Rabbit Anti-BMI1 Antibody (VS-0924-YC21) - Cancer Stem Cell Marker (CAT#: VS-0924-YC21)
This anti-BMI1 antibody (VS-0924-YC21) targets the human BMI1 protein, a marker for cancer stem cells (CSCs). It is suitable for detecting BMI1 in CSC tissues or cells by Immunofluorescence (0.5-2 µg/mL).
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line HEK 293
(Immunofluorescent staining of human cell line HEK 293 shows localization to nuclear bodies.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30471/1564_A5_1_selected.jpg
(Immunofluorescent staining of human cell line HEK 293 shows localization to nuclear bodies.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30471/1564_A5_1_selected.jpg
Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30472/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30472/if_selected.jpg
Figure 3 IHC staining of human adrenal gland
(Immunohistochemical staining of human adrenal gland shows strong nuclear positivity in cortical cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11120/ihc_selected.jpg
(Immunohistochemical staining of human adrenal gland shows strong nuclear positivity in cortical cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11120/ihc_selected.jpg
Figure 4 Testis
(Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: Medium Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11120/34651_A_6_6.jpg
(Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: Medium Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11120/34651_A_6_6.jpg
Figure 5 Lymph node
(Germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11120/34651_A_8_8.jpg
(Germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11120/34651_A_8_8.jpg
Figure 6 Cerebral cortex
(Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11120/34651_B_8_5.jpg
(Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11120/34651_B_8_5.jpg
Figure 7 Nasopharynx
(Respiratory epithelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11120/34651_B_8_2.jpg
(Respiratory epithelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11120/34651_B_8_2.jpg
Figure 8 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11120/34651_A_7_3.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11120/34651_A_7_3.jpg
Figure 9 Kidney
(Collecting ducts Staining: Medium Intensity: Strong Quantity: <25% Distal tubules Staining: High Intensity: Strong Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11120/34651_A_7_5.jpg
(Collecting ducts Staining: Medium Intensity: Strong Quantity: <25% Distal tubules Staining: High Intensity: Strong Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11120/34651_A_7_5.jpg
Figure 10 RNA cell line category: Cell line enhanced (HDLM-2, U-937)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000168283-BMI1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000168283-BMI1
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human BMI1
- Species Reactivity
- Human
- Applications
- IF
Product Property
- Clonality
- Polyclonal
- Purification
- Affinity purified using the PrEST antigen as affinity ligand
- Concentration
- 0.05 mg/mL
- Buffer
- PBS, pH 7.2, with 40% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Immunofluorescence (IF): 0.5-2 µg/mL
Target
- Alternative Names
- PCGF4; RNF51
- Gene ID
- 648
- UniProt ID
- P35226
- Long Name
- BMI1 proto-oncogene, polycomb ring finger
- Cellular Localization
- Cytoplasm, Nucleus
- Function
- Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.
The complex composed of RNF2, UB2D3 and BMI1 binds nucleosomes, and has activity only with nucleosomal histone H2A.
In the PRC1-like complex, regulates the E3 ubiquitin-protein ligase activity of RNF2/RING2.
Recommended Products
Isotype Control
Secondary Antibody
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0365 | Hi-Affi™ Rabbit Anti-BMI1 Recombinant Antibody (clone DS365AB) | WB, IHC, ICC | Rabbit IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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