Hi-Affi™ Rabbit Anti-BMI1 Recombinant Antibody (clone DS365AB)
CAT#: MOR-0365
Recombinant Rabbit anti-BMI1 monoclonal antibody for WB, IHC, ICC, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line HEK 293
Immunofluorescent staining of human cell line HEK 293 shows localization to nuclear bodies.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30471/1564_A5_1_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30472/if_selected.jpg
Normal Tissue
Figure 3 IHC staining of human adrenal gland
Immunohistochemical staining of human adrenal gland shows strong nuclear positivity in cortical cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11120/ihc_selected.jpg
Normal Tissue
Figure 4 Testis
Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: Medium Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11120/34651_A_6_6.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11120/34651_A_8_8.jpg
Normal Tissue
Figure 6 Cerebral cortex
Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11120/34651_B_8_5.jpg
Normal Tissue
Figure 7 Nasopharynx
Respiratory epithelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11120/34651_B_8_2.jpg
Normal Tissue
Figure 8 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11120/34651_A_7_3.jpg
Normal Tissue
Figure 9 Kidney
Collecting ducts Staining: Medium Intensity: Strong Quantity: <25% Distal tubules Staining: High Intensity: Strong Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11120/34651_A_7_5.jpg
RNA Expression
Figure 10 RNA cell line category: Cell line enhanced (HDLM-2, U-937)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000168283-BMI1
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Specifications
- Host Species
- Rabbit
- Derivation
- Phage display library screening
- Type
- Rabbit IgG
- Species Reactivity
- Human
- Clone
- DS365AB
- Applications
- WB, IHC, ICC
- Conjugate
- Unconjugated
Product Property
- Purification
- Purified with Absolute Mag™ Protein A Agarose
- Purity
- >95% as determined by analysis by SDS-PAGE
- Format
- Liquid
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- BMI1 Proto-Oncogene, Polycomb Ring Finger; Polycomb Group RING Finger Protein 4; PCGF4; RNF51; B Lymphoma Mo-MLV Insertion Region 1 Homolog (Mouse); Murine Leukemia Viral (Bmi-1) Oncogene Homolog; B Lymphoma Mo-MLV Insertion Region 1 Homolog; BMI1 Polycomb Ring Finger Proto-Oncogene; BMI1 Polycomb Ring Finger Oncogene; Polycomb Group Ring Finger 4; Polycomb Group Protein Bmi1; Ring Finger Protein 51; RING Finger Protein 51; Flvi-2/Bmi-1; FLVI2/BMI1
- Gene ID
- 648
- UniProt ID
- P35226
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "BMI1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0263CT | Mouse Anti-BMI1 Recombinant Antibody (clone EML250) | WB | Mouse IgG1 |
| MRO-0184-CN | Mouse Anti-BMI1 Recombinant Antibody (clone A11-C11) | WB, IHC, IF | Mouse IgG2a |
| MRO-0185-CN | Rabbit Anti-BMI1 Recombinant Antibody (clone CBACN-072) | WB, IF, IHC | Rabbit IgG |
| MRO-0186-CN | Mouse Anti-BMI1 Recombinant Antibody (clone B3-G5) | WB, IF, IHC, FC | Mouse IgG2a |
| VS3-CJ895 | Mouse Anti-BMI1 Recombinant Antibody (VS3-CJ895) | WB, ICC | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN0449 | PE-A*02:01/Human BMI1 (TLQDIVYKL) MHC Tetramer | FCM, IHC | |
| MHC-CN0450 | APC-A*02:01/Human BMI1 (TLQDIVYKL) MHC Tetramer | FCM, IHC | |
| MHC-CN0451 | FITC-A*02:01/Human BMI1 (TLQDIVYKL) MHC Tetramer | FCM, IHC | |
| MHC-CN0452 | A*02:01/Human BMI1 (TLQDIVYKL) MHC Tetramer | FCM, IHC | |
| MHC-CN0453 | PE-A*02:01/Human BMI1 (CLPSPSTPV) MHC Tetramer | FCM, IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY31 | AbPlus™ Anti-BMI1 Magnetic Beads (229F6) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0924-YC21 | Rabbit Anti-BMI1 Antibody (VS-0924-YC21) - Cancer Stem Cell Marker | IF | Rabbit IgG |
| VS-0924-YC22 | Rabbit Anti-BMI1 Antibody (VS-0924-YC22) - Cancer Stem Cell Marker | IHC, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY249 | Anti-BMI1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY792 | Anti-Mouse BMI1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY791 | Anti-Human BMI1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC101 | CytoStream™ Mouse Anti-BMI1 Recombinant Antibody (VS13-YC101) | WB, ICC, IF, IHC-P, FC | Mouse IgG2a |
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