Rabbit Anti-BMI1 Antibody (VS-0924-YC22) - Cancer Stem Cell Marker
CAT#: VS-0924-YC22
This anti-BMI1 antibody (VS-0924-YC22) targets BMI1 protein in humans, mice, and rats, serving as a marker for cancer stem cells (CSCs). It can be used for detecting BMI1 in CSC tissues or cells by Immunohistochemistry (1/50-1/200) and Western blot (0.1-0.5 µg/mL).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line HEK 293
Immunofluorescent staining of human cell line HEK 293 shows localization to nuclear bodies.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30471/1564_A5_1_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30472/if_selected.jpg
Normal Tissue
Figure 3 IHC staining of human adrenal gland
Immunohistochemical staining of human adrenal gland shows strong nuclear positivity in cortical cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11120/ihc_selected.jpg
Normal Tissue
Figure 4 Testis
Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: Medium Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11120/34651_A_6_6.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11120/34651_A_8_8.jpg
Normal Tissue
Figure 6 Cerebral cortex
Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11120/34651_B_8_5.jpg
Normal Tissue
Figure 7 Nasopharynx
Respiratory epithelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11120/34651_B_8_2.jpg
Normal Tissue
Figure 8 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11120/34651_A_7_3.jpg
Normal Tissue
Figure 9 Kidney
Collecting ducts Staining: Medium Intensity: Strong Quantity: <25% Distal tubules Staining: High Intensity: Strong Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11120/34651_A_7_5.jpg
RNA Expression
Figure 10 RNA cell line category: Cell line enhanced (HDLM-2, U-937)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000168283-BMI1
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat BMI1
- Species Reactivity
- Human, Mouse, Rat
- Applications
- IHC, WB
Product Property
- Clonality
- Polyclonal
- Purification
- Affinity purified using the PrEST antigen as affinity ligand
- Concentration
- 0.2 mg/mL
- Buffer
- PBS, pH 7.2, with 40% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Immunohistochemistry (IHC): 1/50-1/200
Western Blot (WB): 0.1-0.5 µg/mL
Target
- Alternative Names
- PCGF4; RNF51
- Gene ID
- 648
- UniProt ID
- P35226
- Long Name
- BMI1 proto-oncogene, polycomb ring finger
- Cellular Localization
- Cytoplasm, Nucleus
- Function
- Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.
The complex composed of RNF2, UB2D3 and BMI1 binds nucleosomes, and has activity only with nucleosomal histone H2A.
In the PRC1-like complex, regulates the E3 ubiquitin-protein ligase activity of RNF2/RING2.
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Datasheet
MSDS
COA
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "BMI1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-4024z | Mouse Anti-BMI1 Recombinant Antibody (clone 43H11) | WB, FC, ICC, IF, IHC | Mouse IgG1 |
| MOB-0263CT | Mouse Anti-BMI1 Recombinant Antibody (clone EML250) | WB | Mouse IgG1 |
| ZG-0570J | Rabbit Anti-BMI1 Recombinant Antibody (clone 1F2) | ELISA, WB, IHC | Rabbit IgG |
| VS3-CJ895 | Mouse Anti-BMI1 Recombinant Antibody (VS3-CJ895) | WB, ICC | Mouse IgG1, κ |
| VS3-CJ896 | Rabbit Anti-BMI1 Recombinant Antibody (VS3-CJ896) | WB, ICC, IF, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0365 | Hi-Affi™ Rabbit Anti-BMI1 Recombinant Antibody (clone DS365AB) | WB, IHC, ICC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN0449 | PE-A*02:01/Human BMI1 (TLQDIVYKL) MHC Tetramer | FCM, IHC | |
| MHC-CN0450 | APC-A*02:01/Human BMI1 (TLQDIVYKL) MHC Tetramer | FCM, IHC | |
| MHC-CN0451 | FITC-A*02:01/Human BMI1 (TLQDIVYKL) MHC Tetramer | FCM, IHC | |
| MHC-CN0452 | A*02:01/Human BMI1 (TLQDIVYKL) MHC Tetramer | FCM, IHC | |
| MHC-CN0453 | PE-A*02:01/Human BMI1 (CLPSPSTPV) MHC Tetramer | FCM, IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY31 | AbPlus™ Anti-BMI1 Magnetic Beads (229F6) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0924-YC21 | Rabbit Anti-BMI1 Antibody (VS-0924-YC21) - Cancer Stem Cell Marker | IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY249 | Anti-BMI1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY792 | Anti-Mouse BMI1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY791 | Anti-Human BMI1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC101 | CytoStream™ Mouse Anti-BMI1 Recombinant Antibody (VS13-YC101) | WB, ICC, IF, IHC-P, FC | Mouse IgG2a |
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