Rabbit Anti-BTK (pY223) Recombinant Antibody (clone R09-4V4) (CAT#: VS3-WK2119)
This product is a rabbit recombinant antibody that is specific to phosphorylated BTK. The target of this antibody is phospho-BTK (Tyr223). The clone R09-4V4 can be used in WB analysis at a dilution of 1/500-1/1000 and IP analysis at a dilution of 1/20. The molecular weight of BTK (pTyr223) observed in WB experiments using this antibody is 76 kDa.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line HEL
(Immunofluorescent staining of human cell line HEL shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2028/1770_B11_1_selected.jpg
(Immunofluorescent staining of human cell line HEL shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2028/1770_B11_1_selected.jpg
Figure 2 Colon
(Enterocytes - Mucosal lymphoid cells Staining: High Intensity: Strong Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1198/2770_A_9_3.jpg
(Enterocytes - Mucosal lymphoid cells Staining: High Intensity: Strong Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1198/2770_A_9_3.jpg
Figure 3 High expression in lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1198/2770_A_8_8.jpg
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1198/2770_A_8_8.jpg
Figure 4 Spleen
(Cells in red pulp Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in white pulp Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1198/2770_B_8_4.jpg
(Cells in red pulp Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in white pulp Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1198/2770_B_8_4.jpg
Figure 5 RNA cell line category: Cell line enhanced (Daudi, HEL, HMC-1, U-937)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000010671-BTK
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000010671-BTK
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Phospho-Tyr223 of human BTK
- Species Reactivity
- Human
- Clone
- R09-4V4
- Applications
- WB, IP
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% Glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IP: 1/20
Target
- Alternative Names
- Bruton Tyrosine Kinase; Bruton Agammaglobulinemia Tyrosine Kinase; B-Cell Progenitor Kinase; Brutons Tyrosine Kinase; EC 2.7.10.2; AGMX1; ATK; BPK; Tyrosine-Protein Kinase BTK Isoform (Lacking Exon 13 To 17); Dominant-Negative Kinase-Deficient Brutons Tyrosine Kinase; Tyrosine-Protein Kinase BTK Isoform (Lacking Exon 14)
- Gene ID
- 695
- UniProt ID
- Q06187
- Long Name
- Bruton Tyrosine Kinase
Recommended Products
Isotype Control
Secondary Antibody
Related Resources
BCR Signaling Pathway
Primary Immunodeficiency
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "BTK"
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Rabbit Monoclonal Antibody Products
IgG Antibody Products
Antibody Magnetic Beads
Non-Human Primate (NHP) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0393 | Hi-Affi™ Rabbit Anti-BTK Recombinant Antibody (clone DS393AB) | ICC, WB | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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