Mouse Anti-NHP BTK Recombinant Antibody (clone 7F12H4)
CAT#: VS-1024-XY57
This sophisticated mouse-derived monoclonal antibody is engineered to target BTK with high precision, demonstrating reactivity with human and non-human primate samples. The antibody is developed using a purified recombinant fragment of BTK as the immunogen, ensuring exceptional specificity. It is validated for Western blot (WB), immunohistochemistry (IHC), and ELISA, providing versatile and reliable performance across various research applications. This antibody is an invaluable tool for investigating BTK expression and function, offering high accuracy and consistency in experimental results.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line HEL
Immunofluorescent staining of human cell line HEL shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2028/1770_B11_1_selected.jpg
Normal Tissue
Figure 2 Colon
Enterocytes - Mucosal lymphoid cells Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1198/2770_A_9_3.jpg
Normal Tissue
Figure 3 High expression in lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1198/2770_A_8_8.jpg
Normal Tissue
Figure 4 Spleen
Cells in red pulp Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in white pulp Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1198/2770_B_8_4.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (Daudi, HEL, HMC-1, U-937)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000010671-BTK
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Specifications
- Immunogen
- Purified recombinant fragment of BTK
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- BTK
- Species Reactivity
- Human, Non-human primate
- Clone
- 7F12H4
- Applications
- WB, IHC, ELISA
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:500-1:1000, IHC: 1:200-1:800, ELISA: 1:8000
Target
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Related Signaling Pathways
BCR Signaling Pathway
Related Diseases
Primary Immunodeficiency
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Recommended Dilution Buffer
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "Clone 7F12H4"
- CAT
- Product Name
See other products for "BTK"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2243z | Mouse Anti-BTK Recombinant Antibody (clone 21F12) | WB, FC, ICC, IF, IHC | Mouse IgG2b |
| MOB-112CQ | Mouse Anti-BTK Recombinant Antibody (clone CBLS-194) | WB, ICC | Mouse IgG1 |
| VS3-XY143 | Mouse Anti-BTK Recombinant Antibody (clone 7F12H4) | ELISA, WB, IHC, ICC | Mouse IgG1 |
| VS3-WK2119 | Rabbit Anti-BTK (pY223) Recombinant Antibody (clone R09-4V4) | WB, IP | Rabbit IgG |
| VS3-WK2141 | Rabbit Anti-BTK (pY551) Recombinant Antibody (clone R07-9C1) | WB, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0393 | Hi-Affi™ Rabbit Anti-BTK Recombinant Antibody (clone DS393AB) | ICC, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY34 | AbPlus™ Anti-BTK Magnetic Beads (CBACN-075) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY267 | Anti-BTK Immunohistochemistry Kit | IHC | |
| VS-0525-XY854 | Anti-Mouse BTK Immunohistochemistry Kit | IHC | |
| VS-0525-XY853 | Anti-Human BTK Immunohistochemistry Kit | IHC |
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