Mouse Anti-NHP BTK Recombinant Antibody (clone 7F12H4) (CAT#: VS-1024-XY57)
This sophisticated mouse-derived monoclonal antibody is engineered to target BTK with high precision, demonstrating reactivity with human and non-human primate samples. The antibody is developed using a purified recombinant fragment of BTK as the immunogen, ensuring exceptional specificity. It is validated for Western blot (WB), immunohistochemistry (IHC), and ELISA, providing versatile and reliable performance across various research applications. This antibody is an invaluable tool for investigating BTK expression and function, offering high accuracy and consistency in experimental results.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line HEL
(Immunofluorescent staining of human cell line HEL shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2028/1770_B11_1_selected.jpg
(Immunofluorescent staining of human cell line HEL shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2028/1770_B11_1_selected.jpg
Figure 2 Colon
(Enterocytes - Mucosal lymphoid cells Staining: High Intensity: Strong Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1198/2770_A_9_3.jpg
(Enterocytes - Mucosal lymphoid cells Staining: High Intensity: Strong Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1198/2770_A_9_3.jpg
Figure 3 High expression in lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1198/2770_A_8_8.jpg
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1198/2770_A_8_8.jpg
Figure 4 Spleen
(Cells in red pulp Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in white pulp Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1198/2770_B_8_4.jpg
(Cells in red pulp Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in white pulp Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1198/2770_B_8_4.jpg
Figure 5 RNA cell line category: Cell line enhanced (Daudi, HEL, HMC-1, U-937)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000010671-BTK
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000010671-BTK
Specifications
- Immunogen
- Purified recombinant fragment of BTK
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- BTK
- Species Reactivity
- Human, Non-human primate
- Clone
- 7F12H4
- Applications
- WB, IHC, ELISA
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:500-1:1000, IHC: 1:200-1:800, ELISA: 1:8000
Target
Recommended Products
Recommended Dilution Buffer
Isotype Control
Secondary Antibody
Related Resources
BCR Signaling Pathway
Primary Immunodeficiency
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0393 | Hi-Affi™ Rabbit Anti-BTK Recombinant Antibody (clone DS393AB) | ICC, WB | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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