AbPlus™ Anti-C5 Magnetic Beads (5G1.1CB)

CAT#: VS-0424-XY36

The AbPlus Anti-C5 Magnetic Beads (5G1.1CB) is an innovative affinity resin which is bound with anti-C5 specific antibody (5G1.1CB). The beads were designed for small-scale affinity purification and immunoprecipitation (IP) of C5 protein under native and denaturing conditions.

Gene Expression
Figure 1 RNA cell line category: Cell line enhanced (A549, Hep G2)

Specifications

  • Applications
  • Immunoprecipitation, Protein Purification
  • Matrix
  • Magrose bead (> 50 μmol/mL gel)
  • Bead Ligand
  • Anti-C5 specific antibody (5G1.1CB)
  • Target
  • C5
  • Target Species
  • Human
  • Antibody Clone
  • 5G1.1CB
  • Bead Capacity
  • 20-30 mg/mL binding antibody
  • Bead size
  • 10-37 μm
  • Stability
  • pH 2-14
  • Format
  • 20% Suspension
  • Buffer
  • PBS, pH 7.4, with 1% BSA
  • Preservative
  • 0.03% Proclin 300
  • Storage
  • Stored at 4°C, and is stable for up to 2 years. Do not centrifuge, dry or freeze the magnetic beads.

Applications

  • Application Notes
  • The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume.

Target

  • Introduction
  • This gene encodes a component of the complement system, a part of the innate immune system that plays an important role in inflammation, host homeostasis, and host defense against pathogens. The encoded preproprotein is proteolytically processed to generate multiple protein products, including the C5 alpha chain, C5 beta chain, C5a anaphylatoxin and C5b. The C5 protein is comprised of the C5 alpha and beta chains, which are linked by a disulfide bridge. Cleavage of the alpha chain by a convertase enzyme results in the formation of the C5a anaphylatoxin, which possesses potent spasmogenic and chemotactic activity, and the C5b macromolecular cleavage product, a subunit of the membrane attack complex (MAC). Mutations in this gene cause complement component 5 deficiency, a disease characterized by recurrent bacterial infections. Alternative splicing results in multiple transcript variants.
  • Alternative Names
  • C5D; C5a; C5b; ECLZB; CPAMD4
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