Rabbit Anti-CAMK1 Recombinant Antibody (clone R06-7C4)
CAT#: VS3-FY1632
Clone R06-7C4 is a rabbit recombinant monoclonal antibody that attaches to human CAMK1. It cross-reacts with mouse, rat. This CaMK1 antibody has been rigorously validated and is widely used in western blot analysis of CaMK1 in rat brain, C6, 3T3 lysates.
Tested Data
Gene Expression
WB
Figure 1 Anti-CAMK1 Antibody (VS3-FY1632) in WB.
The CaMK1 expression in rat Brain, C6, 3T3 lysates were detected using the anti-CaMK1 by western blot assay.
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Subcellular Location
Figure 1 IF staining of human cell line HEK 293
Immunofluorescent staining of human cell line HEK 293 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51409/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human stomach, upper
Immunohistochemical staining of human stomach, upper shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51409/ihc_selected.jpg
Normal Tissue
Figure 3 IHC staining of human adrenal gland
Immunohistochemical staining of human adrenal gland shows moderate cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31904/ihc_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Glial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51409/119167_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51409/119167_A_7_3.jpg
Normal Tissue
Figure 6 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51409/119167_A_7_4.jpg
Normal Tissue
Figure 7 Kidney
Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51409/119167_A_7_5.jpg
Normal Tissue
Figure 8 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51409/119167_A_4_6.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enriched (HMC-1)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000134072-CAMK1
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Specifications
- Immunogen
- Synthetic peptide of human CaMKI
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat CAMK1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R06-7C4
- Applications
- Western Blot
- MW
- The calculated weight of the target protein is approximately 41 kDa, while the observed molecular weight stands at 41 kDa.
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- Calcium/Calmodulin Dependent Protein Kinase I; CaM Kinase I Alpha; EC 2.7.11.17; CaMKI-Alpha; CaM-KI; Calcium/Calmodulin-Dependent Protein Kinase Type 1; CaM Kinase I; EC 2.7.11; CAMKI
- Gene ID
- 8536
- UniProt ID
- Q14012
- Sequence Similarities
- Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Phosphorylated by CaMKK1 and CaMKK2 on Thr-177.
- Protein Refseq
- NP_003647.1
- Function
- Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK1 signaling cascade and, upon calcium influx, regulates transcription activators activity, cell cycle, hormone production, cell differentiation, actin filament organization and neurite outgrowth. Recognizes the substrate consensus sequence [MVLIF]-x-R-x2-[ST]-x3-[MVLIF]. Regulates axonal extension and growth cone motility in hippocampal and cerebellar nerve cells. Upon NMDA receptor-mediated Ca²⁺ elevation, promotes dendritic growth in hippocampal neurons and is essential in synapses for full long-term potentiation (LTP) and ERK2-dependent translational activation. Downstream of NMDA receptors, promotes the formation of spines and synapses in hippocampal neurons by phosphorylating ARHGEF7/BETAPIX on 'Ser-694', which results in the enhancement of ARHGEF7 activity and activation of RAC1. Promotes neuronal differentiation and neurite outgrowth by activation and phosphorylation of MARK2 on 'Ser-91', 'Ser-92', 'Ser-93' and 'Ser-294'. Promotes nuclear export of HDAC5 and binding to 14-3-3 by phosphorylation of 'Ser-259' and 'Ser-498' in the regulation of muscle cell differentiation. Regulates NUMB-mediated endocytosis by phosphorylation of NUMB on 'Ser-276' and 'Ser-295'. Involved in the regulation of basal and estrogen-stimulated migration of medulloblastoma cells through ARHGEF7/BETAPIX phosphorylation (By similarity).
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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| MOB-3291z | Mouse Anti-CAMK1 Recombinant Antibody (clone 40G3) | WB, IP, IF, ELISA | Mouse IgG1, κ |
| MOB-0296CT | Mouse Anti-CAMK1 Recombinant Antibody (clone EML819) | WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0445 | Hi-Affi™ Rabbit Anti-CAMK1 Recombinant Antibody (clone DS445AB) | FC, ICC, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| VS-0325-XY299 | Anti-CAMK1 Immunohistochemistry Kit | IHC |
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