Rabbit Anti-CASP3 Recombinant Antibody (clone 2B10)
CAT#: ZG-0012U
The purified Rabbit Anti-CASP3 Recombinant Antibody (clone 2B10) displayed high specificity against the CASP3 protein of human.
Tested Data
Gene Expression
WB
Figure 1 Rabbit Anti-CASP3 Antibody (ZG-0012U) in WB
Positive WB detected in: Hela whole cell lysate, HEK293 whole cell lysate, Ntera-2 whole cell lysate. All lanes: Caspase-3 antibody at 1: 1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 32 kDa
Observed band size: 32, 28 kDa
IHC
Figure 2 Rabbit Anti-CASP3 Antibody (ZG-0012U) in IHC
IHC image of ZG-0012U diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & mitochondria.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2643/2013_E2_2_selected.jpg
Subcellular Location
Figure 2 IHC staining of human adrenal gland
Immunohistochemical staining of human adrenal gland shows strong cytoplasmic positivity in granular pattern in cortical cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/91/ihc_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & mitochondria.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2643/2_C1_1_red_green.jpg
Subcellular Location
Figure 4 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & mitochondria.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2643/3_C1_1_red_green.jpg
Normal Tissue
Figure 5 Colon
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8381/20562_A_9_3.jpg
Normal Tissue
Figure 6 Testis
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Preleptotene spermatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8381/20562_A_6_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8381/20562_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (Karpas-707)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000164305-CASP3
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Specifications
- Immunogen
- A synthesized peptide derived from human Caspase-3
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human CASP3
- Species Reactivity
- Human
- Clone
- 2B10
- Applications
- ELISA, WB, IHC
- Conjugate
- Unconjugated
- Related Disease
- Oropharynx Cancer and Ischemia
Product Property
- Purification
- Affinity purified
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, western blot, Immunohistochemistry. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IHC:1:50-1:200
Target
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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