Rabbit Anti-CASP3 Recombinant Antibody (clone 2B10) (CAT#: ZG-0012U)
The purified Rabbit Anti-CASP3 Recombinant Antibody (clone 2B10) displayed high specificity against the CASP3 protein of human.
Specific Inquiry
WB
Figure 1 Rabbit Anti-CASP3 Antibody (ZG-0012U) in WB
Positive WB detected in: Hela whole cell lysate, HEK293 whole cell lysate, Ntera-2 whole cell lysate. All lanes: Caspase-3 antibody at 1: 1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 32 kDa
Observed band size: 32, 28 kDa
IHC
Figure 2 Rabbit Anti-CASP3 Antibody (ZG-0012U) in IHC
IHC image of ZG-0012U diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Specifications
- Immunogen
- A synthesized peptide derived from human Caspase-3
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human CASP3
- Species Reactivity
- Human
- Clone
- 2B10
- Applications
- ELISA, WB, IHC
- Conjugate
- Unconjugated
- Related Disease
- Oropharynx Cancer and Ischemia
Product Property
- Purification
- Affinity purified
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, western blot, Immunohistochemistry. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IHC:1:50-1:200
Target
- Alternative Names
- Caspase-3; CASP-3; EC 3.4.22.56; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; SCA-1; Cleaved into: Caspase-3 subunit p17; Caspase-3 subunit p12; CASP3; CPP32
- Gene ID
- 836
- UniProt ID
- P42574
Related Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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Non-Human Primate (NHP) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1296z | Mouse Anti-CASP3 Recombinant Antibody (clone 24A6) | WB, IHC, IF, ELISA | Mouse IgG1 |
| MRO-0241-CN | Rabbit Anti-CASP3 Recombinant Antibody (clone CBACN-090) | WB, IF, IHC, IP, FC | Rabbit IgG |
| ZG-0070J | Mouse Anti-CASP3 Recombinant Antibody (clone 4B8) | IHC-P, IF | Mouse IgG |
| ZG-0071J | Mouse Anti-CASP3 Recombinant Antibody (clone 5H10) | IHC-P, IF | Mouse IgG |
| VS3-QX154 | Mouse Anti-CASP3 Recombinant Antibody (clone 4A3-10B6-7F7) | WB, IHC | Mouse IgG1 |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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