Mouse Anti-CASP3 Recombinant Antibody (clone 5E1) (CAT#: ZG-0016J)
This product is a mouse antibody that recognizes CASP3.
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Custom Production
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IF
Figure 1 Mouse Anti-CASP3 Recombinant Antibody (clone 5E1) in IF
Immunofluorescence analysis of rat-lung tissue. 1.FoxO1 (phospho Ser256) Polyclonal Antibody(red) was diluted at 1:200 (4°C overnight). Active Caspase-3 Monoclonal Antibody (green) was diluted at 1:200 (4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 AS0333 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 AS0784 was diluted at 1:1000 (room temperature, 50min)
IF
Figure 2 Mouse Anti-CASP3 Recombinant Antibody (clone 5E1) in IF
Immunofluorescence analysis of rat-lung tissue. 1.Active Caspase-3 Monoclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary Antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI.Picture C: merge of A+B
IHC
Figure 3 Mouse Anti-CASP3 Recombinant Antibody (clone 5E1) in IHC
Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1.Active Caspase-3 Monoclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for Antibody retrieval(>98°C, 20min). 3, Secondary Antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary Antibody only.
IHC
Figure 4 Mouse Anti-CASP3 Recombinant Antibody (clone 5E1) in IHC
Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1.Active Caspase-3 Monoclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for Antibody retrieval(>98°C, 20min). 3, Secondary Antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary Antibody only.
WB
Figure 5 Mouse Anti-CASP3 Recombinant Antibody (clone 5E1) in WB
Western blot analysis of 1) Hela, 2) 3T3, 3) Rat Brain Tissue using Active Caspase-3 Monoclonal Antibody.
WB
Figure 6 Mouse Anti-CASP3 Recombinant Antibody (clone 5E1) in WB
Western Blot analysis of chicken cell lysis using Antibody diluted at 1:1000
Specifications
- Immunogen
- Recombinant Protein of Active Caspase-3
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human CASP3
- Species Reactivity
- Human, Mouse, Rat, Chicken
- Clone
- 5E1
- Applications
- IF/ICC, WB, IHC-P
Product Property
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Immunofluorescence/Immunocytochemistry, Western Blot, Immunohistochemistry-Paraffin.
Target
- Alternative Names
- Caspase 3; Caspase 3, Apoptosis-Related Cysteine Peptidase; Caspase 3, Apoptosis-Related Cysteine Protease; SREBP Cleavage Activity 1; Cysteine Protease CPP32; Protein Yama; EC 3.4.22.56; Apopain; CASP-3
- Gene ID
- 836
- UniProt ID
- P42574
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABG-037 | Recombinant Anti-Human CASP3 VHH Single Domain Antibody | WB, ELISA, IHC, FuncS | Llama VHH |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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