Mouse Anti-CASP3 Recombinant Antibody (clone 5E1) (CAT#: ZG-0016J)

Figure 1 Mouse Anti-CASP3 Recombinant Antibody (clone 5E1) in IF

Immunofluorescence analysis of rat-lung tissue. 1.FoxO1 (phospho Ser256) Polyclonal Antibody(red) was diluted at 1:200 (4°C overnight). Active Caspase-3 Monoclonal Antibody (green) was diluted at 1:200 (4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 AS0333 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 AS0784 was diluted at 1:1000 (room temperature, 50min)

Figure 2 Mouse Anti-CASP3 Recombinant Antibody (clone 5E1) in IF

Immunofluorescence analysis of rat-lung tissue. 1.Active Caspase-3 Monoclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary Antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI.Picture C: merge of A+B

Figure 3 Mouse Anti-CASP3 Recombinant Antibody (clone 5E1) in IHC

Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1.Active Caspase-3 Monoclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for Antibody retrieval(>98°C, 20min). 3, Secondary Antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary Antibody only.

Figure 4 Mouse Anti-CASP3 Recombinant Antibody (clone 5E1) in IHC

Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1.Active Caspase-3 Monoclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for Antibody retrieval(>98°C, 20min). 3, Secondary Antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary Antibody only.

Figure 5 Mouse Anti-CASP3 Recombinant Antibody (clone 5E1) in WB

Western blot analysis of 1) Hela, 2) 3T3, 3) Rat Brain Tissue using Active Caspase-3 Monoclonal Antibody.

Figure 6 Mouse Anti-CASP3 Recombinant Antibody (clone 5E1) in WB

Western Blot analysis of chicken cell lysis using Antibody diluted at 1:1000