Rabbit Anti-CCNB1 Recombinant Antibody (VS3-CJ654)
CAT#: VS3-CJ654
This product is a rabbit antibody that recognizes human CCNB1.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30741/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61448/1671_B6_5_selected.jpg
Normal Tissue
Figure 3 Colon
Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: Medium Intensity: Strong Quantity: <25% Enterocytes -
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3804/10580_A_9_3.jpg
Normal Tissue
Figure 4 Testis
Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Preleptotene spermatocytes Staining: Medium Intensity: Strong Quantity: <25% Spermatogonia cells Staining: Medium Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3804/10580_A_4_6.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3804/10580_A_8_8.jpg
RNA Expression
Figure 6 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000134057-CCNB1
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Specifications
- Immunogen
- Recombinant protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human CCNB1
- Species Reactivity
- Human
- Applications
- WB, ICC, IF, IHC, IP
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 40% Glycerol, 1% BSA, TBS, pH7.4.
- Preservative
- 0.05% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation.
Target
- Alternative Names
- CCNB
- Gene ID
- 891
- UniProt ID
- P14635
- Sequence Similarities
- Belongs to the cyclin family. Cyclin AB subfamily.
- Cellular Localization
- Cytoplasm, Cytoskeleton, Nucleus
- Post Translation Modifications
- Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.
Phosphorylated by PLK1 at Ser-133 on centrosomes during prophase: phosphorylation by PLK1 does not cause nuclear import. Phosphorylation at Ser-147 was also reported to be mediated by PLK1 but Ser-133 seems to be the primary phosphorylation site.
- Protein Refseq
- NP_114172.1
- Function
- Essential for the control of the cell cycle at the G2/M (mitosis) transition.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "CCNB1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0514 | Hi-Affi™ Rabbit Anti-CCNB1 Recombinant Antibody (clone DS514AB) | FC, ICC, IHC-P, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC4322 | PE-B*57:01/Human CCNB1 (FAFVTDNTY) MHC Tetramer | FCM | |
| MHC-LC4323 | PE-A*29:02/Human CCNB1 (FAFVTDNTY) MHC Tetramer | FCM | |
| MHC-LC4324 | PE-B*58:01/Human CCNB1 (FAFVTDNTY) MHC Tetramer | FCM | |
| MHC-LC4410 | PE-A*01:01/Human CCNB1 (CSEYVKDIY) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0410-CN | Mouse Anti-CCNB1 Recombinant Antibody (clone C2-F2) | WB, IF | Mouse IgG1 |
| MRO-0411-CN | Rabbit Anti-CCNB1 Recombinant Antibody (clone CBACN-162) | WB, IF, IHC, IP | Rabbit IgG |
| VS3-QX173 | Mouse Anti-CCNB1 Recombinant Antibody (clone 1B3-3B1-1B4) | IHC | Mouse IgG |
| VS3-QX174 | Mouse Anti-CCNB1 Recombinant Antibody (clone 4G5-3F3-9G9) | IHC | Mouse IgG |
| VS3-XY180 | Mouse Anti-CCNB1 Recombinant Antibody (clone 1B10) | ELISA, WB, IHC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY146 | Mouse Anti-NHP CCNB1 Recombinant Antibody (clone V152) | WB, IHC, IF, IP | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY334 | Anti-CCNB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1081 | Anti-Mouse CCNB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1080 | Anti-Human CCNB1 Immunohistochemistry Kit | IHC |
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