Mouse Anti-CD226 Recombinant Antibody (clone 10E5) (CAT#: FAMAB-1574CQ)

Figure 1 10E5 mAb recognizes CD226.

293 cells were transfected with either pcDNA3.1 encoding CD226 cDNA (293-CD226) or with thevector alone (293-mock). Transiently transfected cells were stained with 10E5 mAb or rat IgG2b isotype control and the surface expression of CD226 was then analyzed by flow cytometry using anti-rat IgG FITC as secondary Ab.

Dardalhon, V., Schubart, A. S., Reddy, J., Meyers, J. H., Monney, L., Sabatos, C. A., ... & Kuchroo, V. K. (2005). CD226 is specifically expressed on the surface of Th1 cells and regulates their expansion and effector functions. The Journal of Immunology, 175(3), 1558-1565.

Figure 2 CD226 is expressed on Th1 but not Th2 or Th0 T cellclones.

T cell clones (Th0, Th1, or Th2 specific) were stained with 10E5mAb hybridoma supernatants or rat IgG2b isotype control Ab. Anti-ratIgG-FITC was used as the secondary Ab. Surface expression of CD226was then analyzed by flow cytometry.

Dardalhon, V., Schubart, A. S., Reddy, J., Meyers, J. H., Monney, L., Sabatos, C. A., ... & Kuchroo, V. K. (2005). CD226 is specifically expressed on the surface of Th1 cells and regulates their expansion and effector functions. The Journal of Immunology, 175(3), 1558-1565.

Figure 3 CD226 is predominantly expressed on Tcells.

Single cell suspensions were obtained from lymphnodes and spleens from naive C57BL/6 mice and they were costained with 10E5 mAb and PE-conjugated Abs to various cell surface markers (CD4, CD8, DX5,CD11b, CD11c, and B220). Surface expression of these markers was determined by flow cytometry. a, CD226 expression on naive CD4 and CD8 T cells. b, CD226expression on anti-CD3 anti-CD28 activated CD4and CD8 T cells. c, CD226 expression on naive non-T cells: B cells (B220), macrophages (CD11b), dendriticcells (CD11c), and NK cells (NK1.1 and DX5).

Dardalhon, V., Schubart, A. S., Reddy, J., Meyers, J. H., Monney, L., Sabatos, C. A., ... & Kuchroo, V. K. (2005). CD226 is specifically expressed on the surface of Th1 cells and regulates their expansion and effector functions. The Journal of Immunology, 175(3), 1558-1565.